Production of scFv-conjugated affinity silk powder by transgenic silkworm technology.
Bombyx mori (silkworm) silk proteins are being utilized as unique biomaterials for medical applications. Chemical modification or post-conjugation of bioactive ligands expand the applicability of silk proteins; however, the processes are elaborate and costly. In this study, we used transgenic silkwo...
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2012-01-01
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doaj-f9b4393a1760479f9f4e8eb40ab8bd342020-11-25T01:42:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3463210.1371/journal.pone.0034632Production of scFv-conjugated affinity silk powder by transgenic silkworm technology.Mitsuru SatoKatsura KojimaChisato SakumaMaria MurakamiEriko ArataniTakato TakenouchiYasushi TamadaHiroshi KitaniBombyx mori (silkworm) silk proteins are being utilized as unique biomaterials for medical applications. Chemical modification or post-conjugation of bioactive ligands expand the applicability of silk proteins; however, the processes are elaborate and costly. In this study, we used transgenic silkworm technology to develop single-chain variable fragment (scFv)-conjugated silk fibroin. The cocoons of the transgenic silkworm contain fibroin L-chain linked with scFv as a fusion protein. After dissolving the cocoons in lithium bromide, the silk solution was dialyzed, concentrated, freeze-dried, and crushed into powder. Immunoprecipitation analyses demonstrate that the scFv domain retains its specific binding activity to the target molecule after multiple processing steps. These results strongly suggest the promise of scFv-conjugated silk fibroin as an alternative affinity reagent, which can be manufactured using transgenic silkworm technology at lower cost than traditional affinity carriers.http://europepmc.org/articles/PMC3319607?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mitsuru Sato Katsura Kojima Chisato Sakuma Maria Murakami Eriko Aratani Takato Takenouchi Yasushi Tamada Hiroshi Kitani |
spellingShingle |
Mitsuru Sato Katsura Kojima Chisato Sakuma Maria Murakami Eriko Aratani Takato Takenouchi Yasushi Tamada Hiroshi Kitani Production of scFv-conjugated affinity silk powder by transgenic silkworm technology. PLoS ONE |
author_facet |
Mitsuru Sato Katsura Kojima Chisato Sakuma Maria Murakami Eriko Aratani Takato Takenouchi Yasushi Tamada Hiroshi Kitani |
author_sort |
Mitsuru Sato |
title |
Production of scFv-conjugated affinity silk powder by transgenic silkworm technology. |
title_short |
Production of scFv-conjugated affinity silk powder by transgenic silkworm technology. |
title_full |
Production of scFv-conjugated affinity silk powder by transgenic silkworm technology. |
title_fullStr |
Production of scFv-conjugated affinity silk powder by transgenic silkworm technology. |
title_full_unstemmed |
Production of scFv-conjugated affinity silk powder by transgenic silkworm technology. |
title_sort |
production of scfv-conjugated affinity silk powder by transgenic silkworm technology. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
Bombyx mori (silkworm) silk proteins are being utilized as unique biomaterials for medical applications. Chemical modification or post-conjugation of bioactive ligands expand the applicability of silk proteins; however, the processes are elaborate and costly. In this study, we used transgenic silkworm technology to develop single-chain variable fragment (scFv)-conjugated silk fibroin. The cocoons of the transgenic silkworm contain fibroin L-chain linked with scFv as a fusion protein. After dissolving the cocoons in lithium bromide, the silk solution was dialyzed, concentrated, freeze-dried, and crushed into powder. Immunoprecipitation analyses demonstrate that the scFv domain retains its specific binding activity to the target molecule after multiple processing steps. These results strongly suggest the promise of scFv-conjugated silk fibroin as an alternative affinity reagent, which can be manufactured using transgenic silkworm technology at lower cost than traditional affinity carriers. |
url |
http://europepmc.org/articles/PMC3319607?pdf=render |
work_keys_str_mv |
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