The effect of bee pollen on bone biomechanical strength and trabecular bone histomorphometry in tibia of young Japanese quail (Coturnix japonica).
It has been demonstrated in numerous studies that bee pollen supplementation shows numerous positive effects on health. However, its impact on bones is largely unknown. The purpose of this study was to investigate the effect of bee pollen supplementation on the tibia biomechanical properties and bon...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2020-01-01
|
Series: | PLoS ONE |
Online Access: | https://doi.org/10.1371/journal.pone.0230240 |
id |
doaj-f9e28f9f363a46a0ad1aedf04a703a5a |
---|---|
record_format |
Article |
spelling |
doaj-f9e28f9f363a46a0ad1aedf04a703a5a2021-03-03T21:36:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01153e023024010.1371/journal.pone.0230240The effect of bee pollen on bone biomechanical strength and trabecular bone histomorphometry in tibia of young Japanese quail (Coturnix japonica).Ewa TomaszewskaSebastian KnagaPiotr DobrowolskiKrzysztof LamorskiMirosław JabłońskiAgnieszka Tomczyk-WarunekMohammed Jard KadhimMonika Hułas-StasiakGrzegorz BorsukSiemowit MuszyńskiIt has been demonstrated in numerous studies that bee pollen supplementation shows numerous positive effects on health. However, its impact on bones is largely unknown. The purpose of this study was to investigate the effect of bee pollen supplementation on the tibia biomechanical properties and bone morphometric measures using Japanese quail as an animal model. The experiment was arranged in a 2x2x2 factorial design, with sex, quail line (meat-type or egg-lying type), and bee pollen inclusion (0 or 10 g/kg of feed) as factors. The quails were one-day-old at the beginning of the experiment, they were euthanized after 42 days. Our study showed for the first time unfavorable effects of bee pollen on bones properties. Bee pollen supplementation negatively affected bone structure, irrespective of quails' sex or line type. Bone length (P < 0.001), weight (P < 0.01), and mean relative wall thickness (P < 0.01) and mineralization (P < 0.05) were reduced by bee pollen treatment. For female quails, irrespective of line type, the decrease of yield load (P < 0.001), ultimate load (P < 0.01), yield stress (P < 0.001) and ultimate stress (P < 0.05) was noted. Analysis of growth plate in bone metaphysis showed that bee pollen supplementation slowed the process of bone maturation irrespective of sex (P < 0.05). On contrary, dietary bee pollen positively affected bone homeostasis of trabecular bone in bone metaphysis as bone mineral density increased in experimental groups (P < 0.05). In males, this was the result of the increase of trabecular thickness (P < 0.01), in females due to the reduction of trabecular space (P < 0.001). In conclusion, our results demonstrate that bee pollen (1.0%, 10 g/kg of feed) supplementation caused significant negative effects on the mechanical endurance of the tibia of quails, while showed beneficial effects on trabecular bone histomorphometry.https://doi.org/10.1371/journal.pone.0230240 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ewa Tomaszewska Sebastian Knaga Piotr Dobrowolski Krzysztof Lamorski Mirosław Jabłoński Agnieszka Tomczyk-Warunek Mohammed Jard Kadhim Monika Hułas-Stasiak Grzegorz Borsuk Siemowit Muszyński |
spellingShingle |
Ewa Tomaszewska Sebastian Knaga Piotr Dobrowolski Krzysztof Lamorski Mirosław Jabłoński Agnieszka Tomczyk-Warunek Mohammed Jard Kadhim Monika Hułas-Stasiak Grzegorz Borsuk Siemowit Muszyński The effect of bee pollen on bone biomechanical strength and trabecular bone histomorphometry in tibia of young Japanese quail (Coturnix japonica). PLoS ONE |
author_facet |
Ewa Tomaszewska Sebastian Knaga Piotr Dobrowolski Krzysztof Lamorski Mirosław Jabłoński Agnieszka Tomczyk-Warunek Mohammed Jard Kadhim Monika Hułas-Stasiak Grzegorz Borsuk Siemowit Muszyński |
author_sort |
Ewa Tomaszewska |
title |
The effect of bee pollen on bone biomechanical strength and trabecular bone histomorphometry in tibia of young Japanese quail (Coturnix japonica). |
title_short |
The effect of bee pollen on bone biomechanical strength and trabecular bone histomorphometry in tibia of young Japanese quail (Coturnix japonica). |
title_full |
The effect of bee pollen on bone biomechanical strength and trabecular bone histomorphometry in tibia of young Japanese quail (Coturnix japonica). |
title_fullStr |
The effect of bee pollen on bone biomechanical strength and trabecular bone histomorphometry in tibia of young Japanese quail (Coturnix japonica). |
title_full_unstemmed |
The effect of bee pollen on bone biomechanical strength and trabecular bone histomorphometry in tibia of young Japanese quail (Coturnix japonica). |
title_sort |
effect of bee pollen on bone biomechanical strength and trabecular bone histomorphometry in tibia of young japanese quail (coturnix japonica). |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2020-01-01 |
description |
It has been demonstrated in numerous studies that bee pollen supplementation shows numerous positive effects on health. However, its impact on bones is largely unknown. The purpose of this study was to investigate the effect of bee pollen supplementation on the tibia biomechanical properties and bone morphometric measures using Japanese quail as an animal model. The experiment was arranged in a 2x2x2 factorial design, with sex, quail line (meat-type or egg-lying type), and bee pollen inclusion (0 or 10 g/kg of feed) as factors. The quails were one-day-old at the beginning of the experiment, they were euthanized after 42 days. Our study showed for the first time unfavorable effects of bee pollen on bones properties. Bee pollen supplementation negatively affected bone structure, irrespective of quails' sex or line type. Bone length (P < 0.001), weight (P < 0.01), and mean relative wall thickness (P < 0.01) and mineralization (P < 0.05) were reduced by bee pollen treatment. For female quails, irrespective of line type, the decrease of yield load (P < 0.001), ultimate load (P < 0.01), yield stress (P < 0.001) and ultimate stress (P < 0.05) was noted. Analysis of growth plate in bone metaphysis showed that bee pollen supplementation slowed the process of bone maturation irrespective of sex (P < 0.05). On contrary, dietary bee pollen positively affected bone homeostasis of trabecular bone in bone metaphysis as bone mineral density increased in experimental groups (P < 0.05). In males, this was the result of the increase of trabecular thickness (P < 0.01), in females due to the reduction of trabecular space (P < 0.001). In conclusion, our results demonstrate that bee pollen (1.0%, 10 g/kg of feed) supplementation caused significant negative effects on the mechanical endurance of the tibia of quails, while showed beneficial effects on trabecular bone histomorphometry. |
url |
https://doi.org/10.1371/journal.pone.0230240 |
work_keys_str_mv |
AT ewatomaszewska theeffectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT sebastianknaga theeffectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT piotrdobrowolski theeffectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT krzysztoflamorski theeffectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT mirosławjabłonski theeffectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT agnieszkatomczykwarunek theeffectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT mohammedjardkadhim theeffectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT monikahułasstasiak theeffectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT grzegorzborsuk theeffectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT siemowitmuszynski theeffectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT ewatomaszewska effectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT sebastianknaga effectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT piotrdobrowolski effectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT krzysztoflamorski effectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT mirosławjabłonski effectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT agnieszkatomczykwarunek effectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT mohammedjardkadhim effectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT monikahułasstasiak effectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT grzegorzborsuk effectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica AT siemowitmuszynski effectofbeepollenonbonebiomechanicalstrengthandtrabecularbonehistomorphometryintibiaofyoungjapanesequailcoturnixjaponica |
_version_ |
1714816086319300608 |