Comparative study of the pathological effects of western equine encephalomyelitis virus in four strains of Culex tarsalis Coquillett (Diptera: Culicidae)
Early reports suggested that mosquito cells infected with arboviruses remain viable and undamaged. However, more recent experimental evidence suggests that arboviral infection of mosquito tissues might indeed result in pathological changes, with potential implications for vector survival and virus t...
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Frontiers Media S.A.
2014-10-01
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doaj-f9e4cc32cb044a77a26ffe7566c333b52020-11-24T22:53:47ZengFrontiers Media S.A.Frontiers in Public Health2296-25652014-10-01210.3389/fpubh.2014.00184110223Comparative study of the pathological effects of western equine encephalomyelitis virus in four strains of Culex tarsalis Coquillett (Diptera: Culicidae)Marco eNeira0Marco eNeira1Farida eMahmood2William K. Reisen3Calvin B. L. James4William S. Romoser5Pontificia Universidad Catolica del EcuadorOhio UniversityDepartment of the ArmyUniversity of CaliforniaOhio UniversityOhio UniversityEarly reports suggested that mosquito cells infected with arboviruses remain viable and undamaged. However, more recent experimental evidence suggests that arboviral infection of mosquito tissues might indeed result in pathological changes, with potential implications for vector survival and virus transmission. Here, we compare the pathological effects of western equine encephalomyelitis virus (WEEV) infection in four strains of Culex tarsalis previously reported to differ in their competence as WEEV vectors. Pathological effects were observed in cells of the midgut epithelium, salivary glands and eggs. Cell rounding and sloughing of midgut epithelial cells was associated with those strains reported to be the least susceptible to WEEV infection, whereas midgut necrosis and vacuolation upon infection were associated with strains showing higher susceptibility. Although pathological effects were sporadically observed in infected salivary glands, further studies are required to evaluate their impact on vector competence. Additionally, the potential implications of observed Cx. tarsalis egg infection with WEEV are discussed.http://journal.frontiersin.org/Journal/10.3389/fpubh.2014.00184/fullPathologymosquitoArbovirusvector competenceCulex tarsaliswestern equine encephalomyelitis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marco eNeira Marco eNeira Farida eMahmood William K. Reisen Calvin B. L. James William S. Romoser |
spellingShingle |
Marco eNeira Marco eNeira Farida eMahmood William K. Reisen Calvin B. L. James William S. Romoser Comparative study of the pathological effects of western equine encephalomyelitis virus in four strains of Culex tarsalis Coquillett (Diptera: Culicidae) Frontiers in Public Health Pathology mosquito Arbovirus vector competence Culex tarsalis western equine encephalomyelitis |
author_facet |
Marco eNeira Marco eNeira Farida eMahmood William K. Reisen Calvin B. L. James William S. Romoser |
author_sort |
Marco eNeira |
title |
Comparative study of the pathological effects of western equine encephalomyelitis virus in four strains of Culex tarsalis Coquillett (Diptera: Culicidae) |
title_short |
Comparative study of the pathological effects of western equine encephalomyelitis virus in four strains of Culex tarsalis Coquillett (Diptera: Culicidae) |
title_full |
Comparative study of the pathological effects of western equine encephalomyelitis virus in four strains of Culex tarsalis Coquillett (Diptera: Culicidae) |
title_fullStr |
Comparative study of the pathological effects of western equine encephalomyelitis virus in four strains of Culex tarsalis Coquillett (Diptera: Culicidae) |
title_full_unstemmed |
Comparative study of the pathological effects of western equine encephalomyelitis virus in four strains of Culex tarsalis Coquillett (Diptera: Culicidae) |
title_sort |
comparative study of the pathological effects of western equine encephalomyelitis virus in four strains of culex tarsalis coquillett (diptera: culicidae) |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Public Health |
issn |
2296-2565 |
publishDate |
2014-10-01 |
description |
Early reports suggested that mosquito cells infected with arboviruses remain viable and undamaged. However, more recent experimental evidence suggests that arboviral infection of mosquito tissues might indeed result in pathological changes, with potential implications for vector survival and virus transmission. Here, we compare the pathological effects of western equine encephalomyelitis virus (WEEV) infection in four strains of Culex tarsalis previously reported to differ in their competence as WEEV vectors. Pathological effects were observed in cells of the midgut epithelium, salivary glands and eggs. Cell rounding and sloughing of midgut epithelial cells was associated with those strains reported to be the least susceptible to WEEV infection, whereas midgut necrosis and vacuolation upon infection were associated with strains showing higher susceptibility. Although pathological effects were sporadically observed in infected salivary glands, further studies are required to evaluate their impact on vector competence. Additionally, the potential implications of observed Cx. tarsalis egg infection with WEEV are discussed. |
topic |
Pathology mosquito Arbovirus vector competence Culex tarsalis western equine encephalomyelitis |
url |
http://journal.frontiersin.org/Journal/10.3389/fpubh.2014.00184/full |
work_keys_str_mv |
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