Viruses Occur Incorporated in Biogenic High-Mg Calcite from Hypersaline Microbial Mats.
Using three different microscopy techniques (epifluorescence, electronic and atomic force microscopy), we showed that high-Mg calcite grains in calcifying microbial mats from the hypersaline lake "La Salada de Chiprana", Spain, contain viruses with a diameter of 50-80 nm. Energy-dispersive...
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doaj-f9edfb2453d2402bb79632e98fa0111b2021-03-03T20:01:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e013055210.1371/journal.pone.0130552Viruses Occur Incorporated in Biogenic High-Mg Calcite from Hypersaline Microbial Mats.Rutger De WitPascale GautretYvan BettarelCécile RoquesChristian MarlièreMichel RamondaThuy Nguyen ThanhHuy Tran QuangThierry BouvierUsing three different microscopy techniques (epifluorescence, electronic and atomic force microscopy), we showed that high-Mg calcite grains in calcifying microbial mats from the hypersaline lake "La Salada de Chiprana", Spain, contain viruses with a diameter of 50-80 nm. Energy-dispersive X-ray spectrometer analysis revealed that they contain nitrogen and phosphorus in a molar ratio of ~9, which is typical for viruses. Nucleic acid staining revealed that they contain DNA or RNA. As characteristic for hypersaline environments, the concentrations of free and attached viruses were high (>10(10) viruses per g of mat). In addition, we showed that acid treatment (dissolution of calcite) resulted in release of viruses into suspension and estimated that there were ~15 × 10(9) viruses per g of calcite. We suggest that virus-mineral interactions are one of the possible ways for the formation of nano-sized structures often described as "nanobacteria" and that viruses may play a role in initiating calcification.https://doi.org/10.1371/journal.pone.0130552 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rutger De Wit Pascale Gautret Yvan Bettarel Cécile Roques Christian Marlière Michel Ramonda Thuy Nguyen Thanh Huy Tran Quang Thierry Bouvier |
spellingShingle |
Rutger De Wit Pascale Gautret Yvan Bettarel Cécile Roques Christian Marlière Michel Ramonda Thuy Nguyen Thanh Huy Tran Quang Thierry Bouvier Viruses Occur Incorporated in Biogenic High-Mg Calcite from Hypersaline Microbial Mats. PLoS ONE |
author_facet |
Rutger De Wit Pascale Gautret Yvan Bettarel Cécile Roques Christian Marlière Michel Ramonda Thuy Nguyen Thanh Huy Tran Quang Thierry Bouvier |
author_sort |
Rutger De Wit |
title |
Viruses Occur Incorporated in Biogenic High-Mg Calcite from Hypersaline Microbial Mats. |
title_short |
Viruses Occur Incorporated in Biogenic High-Mg Calcite from Hypersaline Microbial Mats. |
title_full |
Viruses Occur Incorporated in Biogenic High-Mg Calcite from Hypersaline Microbial Mats. |
title_fullStr |
Viruses Occur Incorporated in Biogenic High-Mg Calcite from Hypersaline Microbial Mats. |
title_full_unstemmed |
Viruses Occur Incorporated in Biogenic High-Mg Calcite from Hypersaline Microbial Mats. |
title_sort |
viruses occur incorporated in biogenic high-mg calcite from hypersaline microbial mats. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
Using three different microscopy techniques (epifluorescence, electronic and atomic force microscopy), we showed that high-Mg calcite grains in calcifying microbial mats from the hypersaline lake "La Salada de Chiprana", Spain, contain viruses with a diameter of 50-80 nm. Energy-dispersive X-ray spectrometer analysis revealed that they contain nitrogen and phosphorus in a molar ratio of ~9, which is typical for viruses. Nucleic acid staining revealed that they contain DNA or RNA. As characteristic for hypersaline environments, the concentrations of free and attached viruses were high (>10(10) viruses per g of mat). In addition, we showed that acid treatment (dissolution of calcite) resulted in release of viruses into suspension and estimated that there were ~15 × 10(9) viruses per g of calcite. We suggest that virus-mineral interactions are one of the possible ways for the formation of nano-sized structures often described as "nanobacteria" and that viruses may play a role in initiating calcification. |
url |
https://doi.org/10.1371/journal.pone.0130552 |
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