An Electrochemical Strategy for the Simultaneous Detection of Doxorubicin and Simvastatin for Their Potential Use in the Treatment of Cancer
The aim of this study was to develop a disposable, simple, fast, and sensitive sensor for the simultaneous electrochemical detection of doxorubicin (DOX) and simvastatin (SMV), which could be used in preclinical studies for the development of new pharmaceutical formulations for drug delivery. Firstl...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-01-01
|
Series: | Biosensors |
Subjects: | |
Online Access: | https://www.mdpi.com/2079-6374/11/1/15 |
id |
doaj-f9f513c959924cf9b74d1a9f48710fbe |
---|---|
record_format |
Article |
spelling |
doaj-f9f513c959924cf9b74d1a9f48710fbe2021-01-04T00:01:47ZengMDPI AGBiosensors2079-63742021-01-0111151510.3390/bios11010015An Electrochemical Strategy for the Simultaneous Detection of Doxorubicin and Simvastatin for Their Potential Use in the Treatment of CancerIulia Rus0Mihaela Tertiș1Cristina Barbălată2Alina Porfire3Ioan Tomuță4Robert Săndulescu5Cecilia Cristea6Department of Analytical Chemistry, Iuliu Hațieganu University of Medicine and Pharmacy, 4 Louis Pasteur Street, 400349 Cluj-Napoca, RomaniaDepartment of Analytical Chemistry, Iuliu Hațieganu University of Medicine and Pharmacy, 4 Louis Pasteur Street, 400349 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Technology and Biopharmaceutics Iuliu Hațieganu University of Medicine and Pharmacy, 41 Victor Babes Street, 400012 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Technology and Biopharmaceutics Iuliu Hațieganu University of Medicine and Pharmacy, 41 Victor Babes Street, 400012 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Technology and Biopharmaceutics Iuliu Hațieganu University of Medicine and Pharmacy, 41 Victor Babes Street, 400012 Cluj-Napoca, RomaniaDepartment of Analytical Chemistry, Iuliu Hațieganu University of Medicine and Pharmacy, 4 Louis Pasteur Street, 400349 Cluj-Napoca, RomaniaDepartment of Analytical Chemistry, Iuliu Hațieganu University of Medicine and Pharmacy, 4 Louis Pasteur Street, 400349 Cluj-Napoca, RomaniaThe aim of this study was to develop a disposable, simple, fast, and sensitive sensor for the simultaneous electrochemical detection of doxorubicin (DOX) and simvastatin (SMV), which could be used in preclinical studies for the development of new pharmaceutical formulations for drug delivery. Firstly, the electrochemical behavior of each molecule was analyzed regarding the influence of electrode material, electrolyte solution, and scan rate. After this, the proper electrode material, electrolyte solution, and scan rate for both active substances were chosen, and a linear sweep voltammetry procedure was optimized for simultaneous detection. Two chronoamperometry procedures were tested, one for the detection of DOX in the presence of SMV, and the other one for the detection of DOX and SMV together. Finally, calibration curves for DOX and SMV in the presence of each other were obtained using both electrochemical methods and the results were compared. The use of amperometry allowed for a better limit of detection (DOX: 0.1 μg/mL; SMV: 0.7 μg/mL) than the one obtained in voltammetry (1.5 μg/mL for both drugs). The limits of quantification using amperometry were 0.5 μg/mL for DOX (dynamic range: 0.5–65 μg/mL) and 2 μg/mL for SMV (dynamic range: 2–65 μg/mL), while using voltammetry 1 μg/mL was obtained for DOX (dynamic range: 1–100 μg/mL) and 5 μg/mL for SMV (dynamic range: 5–100 μg/mL). This detection strategy represents a promising tool for the analysis of new pharmaceutical formulations for targeted drug delivery containing both drugs, whose association was proven to bring benefits in the treatment of cancer.https://www.mdpi.com/2079-6374/11/1/15doxorubicinsimvastatinelectrochemical simultaneous detectionpharmaceutical analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Iulia Rus Mihaela Tertiș Cristina Barbălată Alina Porfire Ioan Tomuță Robert Săndulescu Cecilia Cristea |
spellingShingle |
Iulia Rus Mihaela Tertiș Cristina Barbălată Alina Porfire Ioan Tomuță Robert Săndulescu Cecilia Cristea An Electrochemical Strategy for the Simultaneous Detection of Doxorubicin and Simvastatin for Their Potential Use in the Treatment of Cancer Biosensors doxorubicin simvastatin electrochemical simultaneous detection pharmaceutical analysis |
author_facet |
Iulia Rus Mihaela Tertiș Cristina Barbălată Alina Porfire Ioan Tomuță Robert Săndulescu Cecilia Cristea |
author_sort |
Iulia Rus |
title |
An Electrochemical Strategy for the Simultaneous Detection of Doxorubicin and Simvastatin for Their Potential Use in the Treatment of Cancer |
title_short |
An Electrochemical Strategy for the Simultaneous Detection of Doxorubicin and Simvastatin for Their Potential Use in the Treatment of Cancer |
title_full |
An Electrochemical Strategy for the Simultaneous Detection of Doxorubicin and Simvastatin for Their Potential Use in the Treatment of Cancer |
title_fullStr |
An Electrochemical Strategy for the Simultaneous Detection of Doxorubicin and Simvastatin for Their Potential Use in the Treatment of Cancer |
title_full_unstemmed |
An Electrochemical Strategy for the Simultaneous Detection of Doxorubicin and Simvastatin for Their Potential Use in the Treatment of Cancer |
title_sort |
electrochemical strategy for the simultaneous detection of doxorubicin and simvastatin for their potential use in the treatment of cancer |
publisher |
MDPI AG |
series |
Biosensors |
issn |
2079-6374 |
publishDate |
2021-01-01 |
description |
The aim of this study was to develop a disposable, simple, fast, and sensitive sensor for the simultaneous electrochemical detection of doxorubicin (DOX) and simvastatin (SMV), which could be used in preclinical studies for the development of new pharmaceutical formulations for drug delivery. Firstly, the electrochemical behavior of each molecule was analyzed regarding the influence of electrode material, electrolyte solution, and scan rate. After this, the proper electrode material, electrolyte solution, and scan rate for both active substances were chosen, and a linear sweep voltammetry procedure was optimized for simultaneous detection. Two chronoamperometry procedures were tested, one for the detection of DOX in the presence of SMV, and the other one for the detection of DOX and SMV together. Finally, calibration curves for DOX and SMV in the presence of each other were obtained using both electrochemical methods and the results were compared. The use of amperometry allowed for a better limit of detection (DOX: 0.1 μg/mL; SMV: 0.7 μg/mL) than the one obtained in voltammetry (1.5 μg/mL for both drugs). The limits of quantification using amperometry were 0.5 μg/mL for DOX (dynamic range: 0.5–65 μg/mL) and 2 μg/mL for SMV (dynamic range: 2–65 μg/mL), while using voltammetry 1 μg/mL was obtained for DOX (dynamic range: 1–100 μg/mL) and 5 μg/mL for SMV (dynamic range: 5–100 μg/mL). This detection strategy represents a promising tool for the analysis of new pharmaceutical formulations for targeted drug delivery containing both drugs, whose association was proven to bring benefits in the treatment of cancer. |
topic |
doxorubicin simvastatin electrochemical simultaneous detection pharmaceutical analysis |
url |
https://www.mdpi.com/2079-6374/11/1/15 |
work_keys_str_mv |
AT iuliarus anelectrochemicalstrategyforthesimultaneousdetectionofdoxorubicinandsimvastatinfortheirpotentialuseinthetreatmentofcancer AT mihaelatertis anelectrochemicalstrategyforthesimultaneousdetectionofdoxorubicinandsimvastatinfortheirpotentialuseinthetreatmentofcancer AT cristinabarbalata anelectrochemicalstrategyforthesimultaneousdetectionofdoxorubicinandsimvastatinfortheirpotentialuseinthetreatmentofcancer AT alinaporfire anelectrochemicalstrategyforthesimultaneousdetectionofdoxorubicinandsimvastatinfortheirpotentialuseinthetreatmentofcancer AT ioantomuta anelectrochemicalstrategyforthesimultaneousdetectionofdoxorubicinandsimvastatinfortheirpotentialuseinthetreatmentofcancer AT robertsandulescu anelectrochemicalstrategyforthesimultaneousdetectionofdoxorubicinandsimvastatinfortheirpotentialuseinthetreatmentofcancer AT ceciliacristea anelectrochemicalstrategyforthesimultaneousdetectionofdoxorubicinandsimvastatinfortheirpotentialuseinthetreatmentofcancer AT iuliarus electrochemicalstrategyforthesimultaneousdetectionofdoxorubicinandsimvastatinfortheirpotentialuseinthetreatmentofcancer AT mihaelatertis electrochemicalstrategyforthesimultaneousdetectionofdoxorubicinandsimvastatinfortheirpotentialuseinthetreatmentofcancer AT cristinabarbalata electrochemicalstrategyforthesimultaneousdetectionofdoxorubicinandsimvastatinfortheirpotentialuseinthetreatmentofcancer AT alinaporfire electrochemicalstrategyforthesimultaneousdetectionofdoxorubicinandsimvastatinfortheirpotentialuseinthetreatmentofcancer AT ioantomuta electrochemicalstrategyforthesimultaneousdetectionofdoxorubicinandsimvastatinfortheirpotentialuseinthetreatmentofcancer AT robertsandulescu electrochemicalstrategyforthesimultaneousdetectionofdoxorubicinandsimvastatinfortheirpotentialuseinthetreatmentofcancer AT ceciliacristea electrochemicalstrategyforthesimultaneousdetectionofdoxorubicinandsimvastatinfortheirpotentialuseinthetreatmentofcancer |
_version_ |
1724350168808030208 |