Roles of indocyanine green clearance test and 13C-methacetin breath test in assessing liver function in acute liver injury: a comparative study

• Main Author: MAO Jingyi
• Format: Article
• Language: zho
• Published: Editorial Department of Journal of Clinical Hepatology 2013-11-01
• Series: Linchuang Gandanbing Zazhi
• Subjects: breath tests; indocyanine green; hepatic insufficiency; galactosamine
• Online Access: http://www.lcgdbzz.org/qk_content.asp?id=5546&ClassID=101495414

ObjectiveTo conduct a comparative study of indocyanine green (ICG) clearance test and 13C-methacetin breath test (13C-MBT) in assessing the liver function in acute liver injury. MethodsForty-eight male Sprague-Dawley rats were randomly divided into control group and 6, 12, 24, 48, and 72 h model groups. The rats in control group were intraperitoneally injected with saline, while the rats in model groups were intraperitoneally injected with D-galactosamine (450 mg/kg) to establish an acute liver injury model. The pathological score of liver tissue, ICG retention rate at 15 minutes (ICG R15), and parameters of 13C-MBT, such as duration to peak of 13CO2 delta over baseline (DOB) curve (Tmax), maximum metabolization velocity (MVmax), cumulative excretion at 120 min (CUM120), and maximum DOB (DOBmax) were monitored. Comparison between groups was made by one-way analysis of variance; pairwise comparison of means between samples was made by SNK-q test; correlation between two variables was analyzed by Pearson′s correlation test. ResultsCompared with the control group, all model groups showed significantly increased pathological scores (P＜0.05) and significantly decreased MVmax, CUM120, and DOBmax (P＜0.05). Pathological score was positively correlated with Tmax (r=0.593, P＜0.05) and negatively correlated with MVmax, CUM120, and DOBmax (r=-0.731, -0.618, and -0.592, P＜0.05 for all). Compared with the control group, the 12, 24, and 48 h model groups had significantly increased ICG R15 (P＜0.05), but there was no significant difference in ICG R15 between the 6, 12 h model group and control group (P＞0.05). ConclusionBoth 13C -MBT and ICG clearance test can be used as the effective tools for monitoring the liver function in acute liver injury. 13C -MBT is more sensitive than ICG clearance test in the early diagnosis of acute liver injury.