Widespread Alterations in Translation Elongation in the Brain of Juvenile Fmr1 Knockout Mice
Summary: FMRP (fragile X mental retardation protein) is a polysome-associated RNA-binding protein encoded by Fmr1 that is lost in fragile X syndrome. Increasing evidence suggests that FMRP regulates both translation initiation and elongation, but the gene specificity of these effects is unclear. To...
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doaj-f9fc70e6a4c04cf7ab5329c18d3285ea2020-11-25T02:13:27ZengElsevierCell Reports2211-12472019-03-01261233133322.e5Widespread Alterations in Translation Elongation in the Brain of Juvenile Fmr1 Knockout MiceSohani Das Sharma0Jordan B. Metz1Hongyu Li2Benjamin D. Hobson3Nicholas Hornstein4David Sulzer5Guomei Tang6Peter A. Sims7Department of Systems Biology, Columbia University Medical Center, New York, NY 10032, USADepartment of Systems Biology, Columbia University Medical Center, New York, NY 10032, USA; Medical Scientist Training Program, Columbia University Medical Center, New York, NY 10032, USADepartment of Neurology, Columbia University Medical Center, New York, NY 10032, USADepartment of Systems Biology, Columbia University Medical Center, New York, NY 10032, USA; Medical Scientist Training Program, Columbia University Medical Center, New York, NY 10032, USADepartment of Systems Biology, Columbia University Medical Center, New York, NY 10032, USA; Medical Scientist Training Program, Columbia University Medical Center, New York, NY 10032, USADepartment of Neurology, Columbia University Medical Center, New York, NY 10032, USA; Department of Psychiatry, Columbia University Medical Center, New York, NY 10032, USA; Department of Pharmacology, Columbia University Medical Center, New York, NY 10032, USA; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, NY 10032, USADepartment of Neurology, Columbia University Medical Center, New York, NY 10032, USADepartment of Systems Biology, Columbia University Medical Center, New York, NY 10032, USA; Department of Biochemistry & Molecular Biophysics, Columbia University Medical Center, New York, NY 10032, USA; Sulzberger Columbia Genome Center, Columbia University Medical Center, New York, NY 10032, USA; Corresponding authorSummary: FMRP (fragile X mental retardation protein) is a polysome-associated RNA-binding protein encoded by Fmr1 that is lost in fragile X syndrome. Increasing evidence suggests that FMRP regulates both translation initiation and elongation, but the gene specificity of these effects is unclear. To elucidate the impact of Fmr1 loss on translation, we utilize ribosome profiling for genome-wide measurements of ribosomal occupancy and positioning in the cortex of 24-day-old Fmr1 knockout mice. We find a remarkably coherent reduction in ribosome footprint abundance per mRNA for previously identified, high-affinity mRNA binding partners of FMRP and an increase for terminal oligopyrimidine (TOP) motif-containing genes canonically controlled by mammalian target of rapamycin-eIF4E-binding protein-eIF4E binding protein-eukaryotic initiation factor 4E (mTOR-4E-BP-eIF4E) signaling. Amino acid motif- and gene-level analyses both show a widespread reduction of translational pausing in Fmr1 knockout mice. Our findings are consistent with a model of FMRP-mediated regulation of both translation initiation through eIF4E and elongation that is disrupted in fragile X syndrome. : Silencing of Fmr1, the gene that encodes FMRP, causes fragile X syndrome. Das Sharma et al. used ribosome profiling in the cortex of 24-day-old Fmr1 knockout mice to dissect FMRP-mediated translational regulation. Fmr1 loss leads to a relief of translational pausing across a large number of genes. Keywords: fragile X syndrome, translational regulation, ribosome profilinghttp://www.sciencedirect.com/science/article/pii/S2211124719302724 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sohani Das Sharma Jordan B. Metz Hongyu Li Benjamin D. Hobson Nicholas Hornstein David Sulzer Guomei Tang Peter A. Sims |
spellingShingle |
Sohani Das Sharma Jordan B. Metz Hongyu Li Benjamin D. Hobson Nicholas Hornstein David Sulzer Guomei Tang Peter A. Sims Widespread Alterations in Translation Elongation in the Brain of Juvenile Fmr1 Knockout Mice Cell Reports |
author_facet |
Sohani Das Sharma Jordan B. Metz Hongyu Li Benjamin D. Hobson Nicholas Hornstein David Sulzer Guomei Tang Peter A. Sims |
author_sort |
Sohani Das Sharma |
title |
Widespread Alterations in Translation Elongation in the Brain of Juvenile Fmr1 Knockout Mice |
title_short |
Widespread Alterations in Translation Elongation in the Brain of Juvenile Fmr1 Knockout Mice |
title_full |
Widespread Alterations in Translation Elongation in the Brain of Juvenile Fmr1 Knockout Mice |
title_fullStr |
Widespread Alterations in Translation Elongation in the Brain of Juvenile Fmr1 Knockout Mice |
title_full_unstemmed |
Widespread Alterations in Translation Elongation in the Brain of Juvenile Fmr1 Knockout Mice |
title_sort |
widespread alterations in translation elongation in the brain of juvenile fmr1 knockout mice |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2019-03-01 |
description |
Summary: FMRP (fragile X mental retardation protein) is a polysome-associated RNA-binding protein encoded by Fmr1 that is lost in fragile X syndrome. Increasing evidence suggests that FMRP regulates both translation initiation and elongation, but the gene specificity of these effects is unclear. To elucidate the impact of Fmr1 loss on translation, we utilize ribosome profiling for genome-wide measurements of ribosomal occupancy and positioning in the cortex of 24-day-old Fmr1 knockout mice. We find a remarkably coherent reduction in ribosome footprint abundance per mRNA for previously identified, high-affinity mRNA binding partners of FMRP and an increase for terminal oligopyrimidine (TOP) motif-containing genes canonically controlled by mammalian target of rapamycin-eIF4E-binding protein-eIF4E binding protein-eukaryotic initiation factor 4E (mTOR-4E-BP-eIF4E) signaling. Amino acid motif- and gene-level analyses both show a widespread reduction of translational pausing in Fmr1 knockout mice. Our findings are consistent with a model of FMRP-mediated regulation of both translation initiation through eIF4E and elongation that is disrupted in fragile X syndrome. : Silencing of Fmr1, the gene that encodes FMRP, causes fragile X syndrome. Das Sharma et al. used ribosome profiling in the cortex of 24-day-old Fmr1 knockout mice to dissect FMRP-mediated translational regulation. Fmr1 loss leads to a relief of translational pausing across a large number of genes. Keywords: fragile X syndrome, translational regulation, ribosome profiling |
url |
http://www.sciencedirect.com/science/article/pii/S2211124719302724 |
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