Experimental Infection of the <i>Biomphalaria glabrata</i> Vector Snail by <i>Schistosoma mansoni</i> Parasites Drives Snail Microbiota Dysbiosis
Host-parasite interaction can result in a strong alteration of the host-associated microbiota. This dysbiosis can affect the fitness of the host; can modify pathogen interaction and the outcome of diseases. <i>Biomphalaria glabrata</i> is the snail intermediate host of the trematode <...
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doaj-fa0277987a0444b8952ad392271ba1fb2021-06-01T00:23:41ZengMDPI AGMicroorganisms2076-26072021-05-0191084108410.3390/microorganisms9051084Experimental Infection of the <i>Biomphalaria glabrata</i> Vector Snail by <i>Schistosoma mansoni</i> Parasites Drives Snail Microbiota DysbiosisAnaïs Portet0Eve Toulza1Ana Lokmer2Camille Huot3David Duval4Richard Galinier5Benjamin Gourbal6IHPE, University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, 66860 Perpignan, FranceIHPE, University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, 66860 Perpignan, FranceLaboratory of Eco-Anthropology UMR 7206 CNRS-MNHN-Paris 7, 75005 Paris, FranceIHPE, University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, 66860 Perpignan, FranceIHPE, University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, 66860 Perpignan, FranceIHPE, University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, 66860 Perpignan, FranceIHPE, University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, 66860 Perpignan, FranceHost-parasite interaction can result in a strong alteration of the host-associated microbiota. This dysbiosis can affect the fitness of the host; can modify pathogen interaction and the outcome of diseases. <i>Biomphalaria glabrata</i> is the snail intermediate host of the trematode <i>Schistosoma mansoni</i>, the agent of human schistosomiasis, causing hundreds of thousands of deaths every year. Here, we present the first study of the snail bacterial microbiota in response to <i>Schistosoma</i> infection. We examined the interplay between <i>B. glabrata, S. mansoni</i> and host microbiota. Snails were infected and the microbiota composition was analysed by 16S rDNA amplicon sequencing approach. We demonstrated that the microbial composition of water did not affect the microbiota composition. Then, we characterised the <i>Biomphalaria</i> bacterial microbiota at the individual scale in both naive and infected snails. Sympatric and allopatric strains of parasites were used for infections and re-infections to analyse the modification or dysbiosis of snail microbiota in different host-parasite co-evolutionary contexts. Concomitantly, using RNAseq, we investigated the link between bacterial microbiota dysbiosis and snail anti-microbial peptide immune response. This work paves the way for a better understanding of snail/schistosome interaction and should have critical consequences in terms of snail control strategies for fighting schistosomiasis disease in the field.https://www.mdpi.com/2076-2607/9/5/1084microbiotabacteria<i>Biomphalaria</i> snail<i>Schistosoma</i> infectionimmune responsedysbiosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anaïs Portet Eve Toulza Ana Lokmer Camille Huot David Duval Richard Galinier Benjamin Gourbal |
spellingShingle |
Anaïs Portet Eve Toulza Ana Lokmer Camille Huot David Duval Richard Galinier Benjamin Gourbal Experimental Infection of the <i>Biomphalaria glabrata</i> Vector Snail by <i>Schistosoma mansoni</i> Parasites Drives Snail Microbiota Dysbiosis Microorganisms microbiota bacteria <i>Biomphalaria</i> snail <i>Schistosoma</i> infection immune response dysbiosis |
author_facet |
Anaïs Portet Eve Toulza Ana Lokmer Camille Huot David Duval Richard Galinier Benjamin Gourbal |
author_sort |
Anaïs Portet |
title |
Experimental Infection of the <i>Biomphalaria glabrata</i> Vector Snail by <i>Schistosoma mansoni</i> Parasites Drives Snail Microbiota Dysbiosis |
title_short |
Experimental Infection of the <i>Biomphalaria glabrata</i> Vector Snail by <i>Schistosoma mansoni</i> Parasites Drives Snail Microbiota Dysbiosis |
title_full |
Experimental Infection of the <i>Biomphalaria glabrata</i> Vector Snail by <i>Schistosoma mansoni</i> Parasites Drives Snail Microbiota Dysbiosis |
title_fullStr |
Experimental Infection of the <i>Biomphalaria glabrata</i> Vector Snail by <i>Schistosoma mansoni</i> Parasites Drives Snail Microbiota Dysbiosis |
title_full_unstemmed |
Experimental Infection of the <i>Biomphalaria glabrata</i> Vector Snail by <i>Schistosoma mansoni</i> Parasites Drives Snail Microbiota Dysbiosis |
title_sort |
experimental infection of the <i>biomphalaria glabrata</i> vector snail by <i>schistosoma mansoni</i> parasites drives snail microbiota dysbiosis |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2021-05-01 |
description |
Host-parasite interaction can result in a strong alteration of the host-associated microbiota. This dysbiosis can affect the fitness of the host; can modify pathogen interaction and the outcome of diseases. <i>Biomphalaria glabrata</i> is the snail intermediate host of the trematode <i>Schistosoma mansoni</i>, the agent of human schistosomiasis, causing hundreds of thousands of deaths every year. Here, we present the first study of the snail bacterial microbiota in response to <i>Schistosoma</i> infection. We examined the interplay between <i>B. glabrata, S. mansoni</i> and host microbiota. Snails were infected and the microbiota composition was analysed by 16S rDNA amplicon sequencing approach. We demonstrated that the microbial composition of water did not affect the microbiota composition. Then, we characterised the <i>Biomphalaria</i> bacterial microbiota at the individual scale in both naive and infected snails. Sympatric and allopatric strains of parasites were used for infections and re-infections to analyse the modification or dysbiosis of snail microbiota in different host-parasite co-evolutionary contexts. Concomitantly, using RNAseq, we investigated the link between bacterial microbiota dysbiosis and snail anti-microbial peptide immune response. This work paves the way for a better understanding of snail/schistosome interaction and should have critical consequences in terms of snail control strategies for fighting schistosomiasis disease in the field. |
topic |
microbiota bacteria <i>Biomphalaria</i> snail <i>Schistosoma</i> infection immune response dysbiosis |
url |
https://www.mdpi.com/2076-2607/9/5/1084 |
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