pMEX01, a 70 kb plasmid isolated from Escherichia coli that confers resistance to multiple β-lactam antibiotics
Abstract Multidrug-resistant microorganisms are of great concern to public health. Genetic mobile elements, such as plasmids, are among the most relevant mechanisms by which bacteria achieve this resistance. We obtained an Escherichia coli strain CM6, isolated from cattle presenting severe diarrheic...
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doaj-fa0c63a3f89c4a94add8c61b5198fe7f2020-11-25T00:55:43ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1678-440549356957410.1016/j.bjm.2017.11.002S1517-83822018000300569pMEX01, a 70 kb plasmid isolated from Escherichia coli that confers resistance to multiple β-lactam antibioticsJose Luis Hernandez-FloresJuan Caballero PérezCarlos Saldaña GutiérrezAndrés Cruz HernándezGerardo Soto AlonsoSergio Pacheco HernándezSergio Romero GómezFrancisco FernándezAchim M. LoskeJuan Campos GuillénAbstract Multidrug-resistant microorganisms are of great concern to public health. Genetic mobile elements, such as plasmids, are among the most relevant mechanisms by which bacteria achieve this resistance. We obtained an Escherichia coli strain CM6, isolated from cattle presenting severe diarrheic symptoms in the State of Querétaro, Mexico. It was found to contain a 70 kb plasmid (pMEX01) with a high similarity to the pHK01-like plasmids that were previously identified and described in Hong Kong. Analysis of the pMEX01 sequence revealed the presence of a blaCTX-M-14 gene, which is responsible for conferring resistance to multiple β-lactam antibiotics. Several genes putatively involved in the conjugative transfer were also identified on the plasmid. The strain CM6 is of high epidemiological concern because it not only displays resistance to multiple β-lactam antibiotics but also to other kinds of antibiotics.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300569&lng=en&tlng=enEscherichia coliPlasmidAntibiotic resistanceblaCTX-M-14 gene |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jose Luis Hernandez-Flores Juan Caballero Pérez Carlos Saldaña Gutiérrez Andrés Cruz Hernández Gerardo Soto Alonso Sergio Pacheco Hernández Sergio Romero Gómez Francisco Fernández Achim M. Loske Juan Campos Guillén |
spellingShingle |
Jose Luis Hernandez-Flores Juan Caballero Pérez Carlos Saldaña Gutiérrez Andrés Cruz Hernández Gerardo Soto Alonso Sergio Pacheco Hernández Sergio Romero Gómez Francisco Fernández Achim M. Loske Juan Campos Guillén pMEX01, a 70 kb plasmid isolated from Escherichia coli that confers resistance to multiple β-lactam antibiotics Brazilian Journal of Microbiology Escherichia coli Plasmid Antibiotic resistance blaCTX-M-14 gene |
author_facet |
Jose Luis Hernandez-Flores Juan Caballero Pérez Carlos Saldaña Gutiérrez Andrés Cruz Hernández Gerardo Soto Alonso Sergio Pacheco Hernández Sergio Romero Gómez Francisco Fernández Achim M. Loske Juan Campos Guillén |
author_sort |
Jose Luis Hernandez-Flores |
title |
pMEX01, a 70 kb plasmid isolated from Escherichia coli that confers resistance to multiple β-lactam antibiotics |
title_short |
pMEX01, a 70 kb plasmid isolated from Escherichia coli that confers resistance to multiple β-lactam antibiotics |
title_full |
pMEX01, a 70 kb plasmid isolated from Escherichia coli that confers resistance to multiple β-lactam antibiotics |
title_fullStr |
pMEX01, a 70 kb plasmid isolated from Escherichia coli that confers resistance to multiple β-lactam antibiotics |
title_full_unstemmed |
pMEX01, a 70 kb plasmid isolated from Escherichia coli that confers resistance to multiple β-lactam antibiotics |
title_sort |
pmex01, a 70 kb plasmid isolated from escherichia coli that confers resistance to multiple β-lactam antibiotics |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1678-4405 |
description |
Abstract Multidrug-resistant microorganisms are of great concern to public health. Genetic mobile elements, such as plasmids, are among the most relevant mechanisms by which bacteria achieve this resistance. We obtained an Escherichia coli strain CM6, isolated from cattle presenting severe diarrheic symptoms in the State of Querétaro, Mexico. It was found to contain a 70 kb plasmid (pMEX01) with a high similarity to the pHK01-like plasmids that were previously identified and described in Hong Kong. Analysis of the pMEX01 sequence revealed the presence of a blaCTX-M-14 gene, which is responsible for conferring resistance to multiple β-lactam antibiotics. Several genes putatively involved in the conjugative transfer were also identified on the plasmid. The strain CM6 is of high epidemiological concern because it not only displays resistance to multiple β-lactam antibiotics but also to other kinds of antibiotics. |
topic |
Escherichia coli Plasmid Antibiotic resistance blaCTX-M-14 gene |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300569&lng=en&tlng=en |
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