Evaluation of Lumbar Intervertebral Disc Degeneration Using T1ρ and T2 Magnetic Resonance Imaging in a Rabbit Disc Injury Model
Study DesignAn in vivo histologic and magnetic resonance imaging (MRI) study of lumbar intervertebral disc (IVD) degeneration was conducted.PurposeTo clarify the sensitivity and efficacy of T1ρ/T2 mapping for IVD degeneration, the correlation between T1ρ/T2 mapping and degenerative grades and histol...
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doaj-fa0de93da99243d0b283ba6fc4e79ca62020-11-24T22:31:24ZengKorean Spine SocietyAsian Spine Journal1976-19021976-78462018-04-0112231732410.4184/asj.2018.12.2.317352Evaluation of Lumbar Intervertebral Disc Degeneration Using T1ρ and T2 Magnetic Resonance Imaging in a Rabbit Disc Injury ModelTetsuhiro Ishikawa0Atsuya Watanabe1Hiroto Kamoda2Masayuki Miyagi3Gen Inoue4Kazuhisa Takahashi5Seiji Ohtori6Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Department of Orthopaedic Surgery, Teikyo University Chiba Medical Center, Ichihara, Japan.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Study DesignAn in vivo histologic and magnetic resonance imaging (MRI) study of lumbar intervertebral disc (IVD) degeneration was conducted.PurposeTo clarify the sensitivity and efficacy of T1ρ/T2 mapping for IVD degeneration, the correlation between T1ρ/T2 mapping and degenerative grades and histological findings in the lumbar IVD were investigated.Overview of LiteratureThe early signs of IVD degeneration are proteoglycan loss, dehydration, and collagen degradation. Recently, several quantitative MRI techniques have been developed; T2 mapping can be used to evaluate hydration and collagen fiber integrity within cartilaginous tissue, and T1ρ mapping can be used to evaluate hydration and proteoglycan content.MethodsUsing New Zealand White rabbits, annular punctures of the IVD were made 10 times at L2/3, 5 times at L3/4, and one time at L4/5 using an 18-gauge needle (n=6) or a 21-gauge needle (n=6). At 4 and 8 weeks post-surgery, MRI was performed including T1ρ and T2 mapping. The degree of IVD degeneration was macroscopically assessed using the Thompson grading system. All specimens were cut for hematoxylin and eosin, safranin-O, and toluidine blue staining.ResultsDisc degeneration became more severe as the number of punctures increased and when the larger needle was used. T1ρ and T2 values were significantly different between grade 1 and grade 3 IVDs, grade 1 and grade 4 IVDs, grade 2 and grade 3 IVDs, and grade 2 and grade 4 IVDs (p<0.05). There was a significant difference between grade 1 and grade 2 IVDs only in terms of T1ρ values (p<0.05).ConclusionsT1ρ and T2 quantitative MRI could detect these small differences. Our results suggest that T1ρ and T2 mapping are sensitive to degenerative changes of lumbar IVDs and that T1ρ mapping can be used as a clinical tool to identify early IVD degeneration.http://www.asianspinejournal.org/upload/pdf/asj-12-317.pdfIntervertebral disc degenerationMagnetic resonance imagingAnimal experimentHistology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tetsuhiro Ishikawa Atsuya Watanabe Hiroto Kamoda Masayuki Miyagi Gen Inoue Kazuhisa Takahashi Seiji Ohtori |
spellingShingle |
Tetsuhiro Ishikawa Atsuya Watanabe Hiroto Kamoda Masayuki Miyagi Gen Inoue Kazuhisa Takahashi Seiji Ohtori Evaluation of Lumbar Intervertebral Disc Degeneration Using T1ρ and T2 Magnetic Resonance Imaging in a Rabbit Disc Injury Model Asian Spine Journal Intervertebral disc degeneration Magnetic resonance imaging Animal experiment Histology |
author_facet |
Tetsuhiro Ishikawa Atsuya Watanabe Hiroto Kamoda Masayuki Miyagi Gen Inoue Kazuhisa Takahashi Seiji Ohtori |
author_sort |
Tetsuhiro Ishikawa |
title |
Evaluation of Lumbar Intervertebral Disc Degeneration Using T1ρ and T2 Magnetic Resonance Imaging in a Rabbit Disc Injury Model |
title_short |
Evaluation of Lumbar Intervertebral Disc Degeneration Using T1ρ and T2 Magnetic Resonance Imaging in a Rabbit Disc Injury Model |
title_full |
Evaluation of Lumbar Intervertebral Disc Degeneration Using T1ρ and T2 Magnetic Resonance Imaging in a Rabbit Disc Injury Model |
title_fullStr |
Evaluation of Lumbar Intervertebral Disc Degeneration Using T1ρ and T2 Magnetic Resonance Imaging in a Rabbit Disc Injury Model |
title_full_unstemmed |
Evaluation of Lumbar Intervertebral Disc Degeneration Using T1ρ and T2 Magnetic Resonance Imaging in a Rabbit Disc Injury Model |
title_sort |
evaluation of lumbar intervertebral disc degeneration using t1ρ and t2 magnetic resonance imaging in a rabbit disc injury model |
publisher |
Korean Spine Society |
series |
Asian Spine Journal |
issn |
1976-1902 1976-7846 |
publishDate |
2018-04-01 |
description |
Study DesignAn in vivo histologic and magnetic resonance imaging (MRI) study of lumbar intervertebral disc (IVD) degeneration was conducted.PurposeTo clarify the sensitivity and efficacy of T1ρ/T2 mapping for IVD degeneration, the correlation between T1ρ/T2 mapping and degenerative grades and histological findings in the lumbar IVD were investigated.Overview of LiteratureThe early signs of IVD degeneration are proteoglycan loss, dehydration, and collagen degradation. Recently, several quantitative MRI techniques have been developed; T2 mapping can be used to evaluate hydration and collagen fiber integrity within cartilaginous tissue, and T1ρ mapping can be used to evaluate hydration and proteoglycan content.MethodsUsing New Zealand White rabbits, annular punctures of the IVD were made 10 times at L2/3, 5 times at L3/4, and one time at L4/5 using an 18-gauge needle (n=6) or a 21-gauge needle (n=6). At 4 and 8 weeks post-surgery, MRI was performed including T1ρ and T2 mapping. The degree of IVD degeneration was macroscopically assessed using the Thompson grading system. All specimens were cut for hematoxylin and eosin, safranin-O, and toluidine blue staining.ResultsDisc degeneration became more severe as the number of punctures increased and when the larger needle was used. T1ρ and T2 values were significantly different between grade 1 and grade 3 IVDs, grade 1 and grade 4 IVDs, grade 2 and grade 3 IVDs, and grade 2 and grade 4 IVDs (p<0.05). There was a significant difference between grade 1 and grade 2 IVDs only in terms of T1ρ values (p<0.05).ConclusionsT1ρ and T2 quantitative MRI could detect these small differences. Our results suggest that T1ρ and T2 mapping are sensitive to degenerative changes of lumbar IVDs and that T1ρ mapping can be used as a clinical tool to identify early IVD degeneration. |
topic |
Intervertebral disc degeneration Magnetic resonance imaging Animal experiment Histology |
url |
http://www.asianspinejournal.org/upload/pdf/asj-12-317.pdf |
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