Oocyte aspiration and in vitro embryo production in Jersey cows with selenium-supplemented diet
The effects of selenium (Se) in Jersey cows' diet on the aspiration of oocytes and production of embryos in vitro were studied. Groups with five Jersey cows received 3.2mg or 9.6mg Se daily, provided in the feed concentrate. Six follicular aspirations were carried out every 15 days, using only...
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Universidade Federal de Minas Gerais
2012-08-01
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doaj-fa118e538dc24044b32acf54236911d12020-11-24T21:49:19ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622012-08-0164478779510.1590/S0102-09352012000400001S0102-09352012000400001Oocyte aspiration and in vitro embryo production in Jersey cows with selenium-supplemented dietG.V. Moraes0J.R. AzevedoT.C. Carneiro1F.L.B. Cavalieri2M. Mataveli3R.M. Rossi4T.C. Santos5Universidade Estadual de MaringáUniversidade Estadual de MaringáCentro Universitário CesumarEmbrapaUniversidade Estadual de MaringáUniversidade Estadual de MaringáThe effects of selenium (Se) in Jersey cows' diet on the aspiration of oocytes and production of embryos in vitro were studied. Groups with five Jersey cows received 3.2mg or 9.6mg Se daily, provided in the feed concentrate. Six follicular aspirations were carried out every 15 days, using only the last 5. The oocytes were classified, and standard procedures were carried out for maturation, fertilization and cultivation. The total number of oocytes (35.11±2.65 vs 23.10±2.16) and degree 1 oocytes (11.61±2.65 vs 4.75±0.97) were higher in the group that received 9.6mg Se and the quantity of naked oocytes (3.23±0.87 vs 6.22±1.18) was lower in this group. The aspirated oocytes from the cows treated with 9.6mg Se/day resulted in higher (P<0.05) embryo production 21.98±2.37 vs 13.12±1.59). No difference was observed in serum Se concentration between the two groups. It is recommended that the daily diet be supplemented with 100g mineral salt containing 9.6mg Se, since this rate rendered a larger production of oocytes, higher quantity of degree 1 oocytes and greater production of embryos in the process of in vitro fertilization.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400001&lng=en&tlng=enbovino de leiteantioxidantefertilização in vitroglutatione peroxidasequalidade de oócitos |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
G.V. Moraes J.R. Azevedo T.C. Carneiro F.L.B. Cavalieri M. Mataveli R.M. Rossi T.C. Santos |
spellingShingle |
G.V. Moraes J.R. Azevedo T.C. Carneiro F.L.B. Cavalieri M. Mataveli R.M. Rossi T.C. Santos Oocyte aspiration and in vitro embryo production in Jersey cows with selenium-supplemented diet Arquivo Brasileiro de Medicina Veterinária e Zootecnia bovino de leite antioxidante fertilização in vitro glutatione peroxidase qualidade de oócitos |
author_facet |
G.V. Moraes J.R. Azevedo T.C. Carneiro F.L.B. Cavalieri M. Mataveli R.M. Rossi T.C. Santos |
author_sort |
G.V. Moraes |
title |
Oocyte aspiration and in vitro embryo production in Jersey cows with selenium-supplemented diet |
title_short |
Oocyte aspiration and in vitro embryo production in Jersey cows with selenium-supplemented diet |
title_full |
Oocyte aspiration and in vitro embryo production in Jersey cows with selenium-supplemented diet |
title_fullStr |
Oocyte aspiration and in vitro embryo production in Jersey cows with selenium-supplemented diet |
title_full_unstemmed |
Oocyte aspiration and in vitro embryo production in Jersey cows with selenium-supplemented diet |
title_sort |
oocyte aspiration and in vitro embryo production in jersey cows with selenium-supplemented diet |
publisher |
Universidade Federal de Minas Gerais |
series |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
issn |
1678-4162 |
publishDate |
2012-08-01 |
description |
The effects of selenium (Se) in Jersey cows' diet on the aspiration of oocytes and production of embryos in vitro were studied. Groups with five Jersey cows received 3.2mg or 9.6mg Se daily, provided in the feed concentrate. Six follicular aspirations were carried out every 15 days, using only the last 5. The oocytes were classified, and standard procedures were carried out for maturation, fertilization and cultivation. The total number of oocytes (35.11±2.65 vs 23.10±2.16) and degree 1 oocytes (11.61±2.65 vs 4.75±0.97) were higher in the group that received 9.6mg Se and the quantity of naked oocytes (3.23±0.87 vs 6.22±1.18) was lower in this group. The aspirated oocytes from the cows treated with 9.6mg Se/day resulted in higher (P<0.05) embryo production 21.98±2.37 vs 13.12±1.59). No difference was observed in serum Se concentration between the two groups. It is recommended that the daily diet be supplemented with 100g mineral salt containing 9.6mg Se, since this rate rendered a larger production of oocytes, higher quantity of degree 1 oocytes and greater production of embryos in the process of in vitro fertilization. |
topic |
bovino de leite antioxidante fertilização in vitro glutatione peroxidase qualidade de oócitos |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400001&lng=en&tlng=en |
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