Single-Cell RNA Sequencing Revealed CD14+ Monocytes Increased in Patients With Takayasu’s Arteritis Requiring Surgical Management
Objectives: Takayasu Arteritis (TA) is a highly specific vascular inflammation and poses threat to patients’ health. Although some patients have accepted medical treatment, their culprit lesions require surgical management (TARSM). This study aimed at dissecting the transcriptomes of peripheral bloo...
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Frontiers Media S.A.
2021-10-01
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Series: | Frontiers in Cell and Developmental Biology |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.761300/full |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gao Qing Gao Qing Gao Qing Gao Qing Wu Zhiyuan Yu Jinge Miao Yuqing Miao Yuqing Chen Zuoguan Diao Yongpeng Yin Jinfeng Yin Jinfeng Jia Junnan Guo Yijia Guo Yijia Li Weimin Li Weimin Li Yongjun Li Yongjun |
spellingShingle |
Gao Qing Gao Qing Gao Qing Gao Qing Wu Zhiyuan Yu Jinge Miao Yuqing Miao Yuqing Chen Zuoguan Diao Yongpeng Yin Jinfeng Yin Jinfeng Jia Junnan Guo Yijia Guo Yijia Li Weimin Li Weimin Li Yongjun Li Yongjun Single-Cell RNA Sequencing Revealed CD14+ Monocytes Increased in Patients With Takayasu’s Arteritis Requiring Surgical Management Frontiers in Cell and Developmental Biology takayasu arteritis single-cell RNA sequencing monocytes CD163 clinical marker |
author_facet |
Gao Qing Gao Qing Gao Qing Gao Qing Wu Zhiyuan Yu Jinge Miao Yuqing Miao Yuqing Chen Zuoguan Diao Yongpeng Yin Jinfeng Yin Jinfeng Jia Junnan Guo Yijia Guo Yijia Li Weimin Li Weimin Li Yongjun Li Yongjun |
author_sort |
Gao Qing |
title |
Single-Cell RNA Sequencing Revealed CD14+ Monocytes Increased in Patients With Takayasu’s Arteritis Requiring Surgical Management |
title_short |
Single-Cell RNA Sequencing Revealed CD14+ Monocytes Increased in Patients With Takayasu’s Arteritis Requiring Surgical Management |
title_full |
Single-Cell RNA Sequencing Revealed CD14+ Monocytes Increased in Patients With Takayasu’s Arteritis Requiring Surgical Management |
title_fullStr |
Single-Cell RNA Sequencing Revealed CD14+ Monocytes Increased in Patients With Takayasu’s Arteritis Requiring Surgical Management |
title_full_unstemmed |
Single-Cell RNA Sequencing Revealed CD14+ Monocytes Increased in Patients With Takayasu’s Arteritis Requiring Surgical Management |
title_sort |
single-cell rna sequencing revealed cd14+ monocytes increased in patients with takayasu’s arteritis requiring surgical management |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2021-10-01 |
description |
Objectives: Takayasu Arteritis (TA) is a highly specific vascular inflammation and poses threat to patients’ health. Although some patients have accepted medical treatment, their culprit lesions require surgical management (TARSM). This study aimed at dissecting the transcriptomes of peripheral blood mononuclear cells (PBMCs) in these patients and to explore potential clinical markers for TA development and progression.Methods: Peripheral blood were collected from four TA patients requiring surgical management and four age-sex matched healthy donors. Single cell RNA sequencing (scRNA-seq) was adopted to explore the transcriptomic diversity and function of their PBMCs. ELISA, qPCR, and FACS were conducted to validate the results of the analysis.Results: A total of 29918 qualified cells were included for downstream analysis. Nine major cell types were confirmed, including CD14+ monocytes, CD8+ T cells, NK cells, CD4+ T cells, B cells, CD16+ monocytes, megakaryocytes, dendritic cells and plasmacytoid dendritic cells. CD14+ monocytes (50.0 vs. 39.3%, p < 0.05) increased in TA patients, as validated by FACS results. TXNIP, AREG, THBS1, and CD163 increased in TA patients. ILs like IL-6, IL-6STP1, IL-6ST, IL-15, and IL-15RA increased in TA group.Conclusion: Transcriptome heterogeneities of PBMCs in TA patients requiring surgical management were revealed in the present study. In the patients with TA, CD14+ monocytes and gene expressions involved in oxidative stress were increased, indicating a new treatment and research direction in this field. |
topic |
takayasu arteritis single-cell RNA sequencing monocytes CD163 clinical marker |
url |
https://www.frontiersin.org/articles/10.3389/fcell.2021.761300/full |
work_keys_str_mv |
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doaj-fa142e7acb3e42b3943150440798cdfb2021-10-04T06:27:10ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-10-01910.3389/fcell.2021.761300761300Single-Cell RNA Sequencing Revealed CD14+ Monocytes Increased in Patients With Takayasu’s Arteritis Requiring Surgical ManagementGao Qing0Gao Qing1Gao Qing2Gao Qing3Wu Zhiyuan4Yu Jinge5Miao Yuqing6Miao Yuqing7Chen Zuoguan8Diao Yongpeng9Yin Jinfeng10Yin Jinfeng11Jia Junnan12Guo Yijia13Guo Yijia14Li Weimin15Li Weimin16Li Yongjun17Li Yongjun18Graduate School of Peking Union Medical College, Chinese Academy of Medical Science, Beijing, ChinaDepartment of Vascular Surgery, National Centre of Gerontology, Beijing Hospital, Beijing, ChinaNational Tuberculosis Clinical Lab of China, Beijing Chest Hospital, Beijing Tuberculosis and Thoracic Tumor Research Institute, Capital Medical University, Beijing, ChinaBeijing Key Laboratory in Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing, ChinaDepartment of Vascular Surgery, National Centre of Gerontology, Beijing Hospital, Beijing, ChinaInstitute of Statistics and Big Data, Renmin University of China, Beijing, ChinaGraduate School of Peking Union Medical College, Chinese Academy of Medical Science, Beijing, ChinaDepartment of Vascular Surgery, National Centre of Gerontology, Beijing Hospital, Beijing, ChinaDepartment of Vascular Surgery, National Centre of Gerontology, Beijing Hospital, Beijing, ChinaDepartment of Vascular Surgery, National Centre of Gerontology, Beijing Hospital, Beijing, ChinaNational Tuberculosis Clinical Lab of China, Beijing Chest Hospital, Beijing Tuberculosis and Thoracic Tumor Research Institute, Capital Medical University, Beijing, ChinaBeijing Key Laboratory in Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing, ChinaBeijing Key Laboratory in Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing, ChinaNational Tuberculosis Clinical Lab of China, Beijing Chest Hospital, Beijing Tuberculosis and Thoracic Tumor Research Institute, Capital Medical University, Beijing, ChinaBeijing Key Laboratory in Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing, ChinaNational Tuberculosis Clinical Lab of China, Beijing Chest Hospital, Beijing Tuberculosis and Thoracic Tumor Research Institute, Capital Medical University, Beijing, ChinaBeijing Key Laboratory in Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing, ChinaGraduate School of Peking Union Medical College, Chinese Academy of Medical Science, Beijing, ChinaDepartment of Vascular Surgery, National Centre of Gerontology, Beijing Hospital, Beijing, ChinaObjectives: Takayasu Arteritis (TA) is a highly specific vascular inflammation and poses threat to patients’ health. Although some patients have accepted medical treatment, their culprit lesions require surgical management (TARSM). This study aimed at dissecting the transcriptomes of peripheral blood mononuclear cells (PBMCs) in these patients and to explore potential clinical markers for TA development and progression.Methods: Peripheral blood were collected from four TA patients requiring surgical management and four age-sex matched healthy donors. Single cell RNA sequencing (scRNA-seq) was adopted to explore the transcriptomic diversity and function of their PBMCs. ELISA, qPCR, and FACS were conducted to validate the results of the analysis.Results: A total of 29918 qualified cells were included for downstream analysis. Nine major cell types were confirmed, including CD14+ monocytes, CD8+ T cells, NK cells, CD4+ T cells, B cells, CD16+ monocytes, megakaryocytes, dendritic cells and plasmacytoid dendritic cells. CD14+ monocytes (50.0 vs. 39.3%, p < 0.05) increased in TA patients, as validated by FACS results. TXNIP, AREG, THBS1, and CD163 increased in TA patients. ILs like IL-6, IL-6STP1, IL-6ST, IL-15, and IL-15RA increased in TA group.Conclusion: Transcriptome heterogeneities of PBMCs in TA patients requiring surgical management were revealed in the present study. In the patients with TA, CD14+ monocytes and gene expressions involved in oxidative stress were increased, indicating a new treatment and research direction in this field.https://www.frontiersin.org/articles/10.3389/fcell.2021.761300/fulltakayasu arteritissingle-cell RNA sequencingmonocytesCD163clinical marker |