A Single Cell but Many Different Transcripts: A Journey into the World of Long Non-Coding RNAs
In late 2012 it was evidenced that most of the human genome is transcribed but only a small percentage of the transcripts are translated. This observation supported the importance of non-coding RNAs and it was confirmed in several organisms. The most abundant non-translated transcripts are long non-...
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doaj-fa1eadaa82254158ab09ac1ee63b1d5a2020-11-25T01:49:14ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-01-0121130210.3390/ijms21010302ijms21010302A Single Cell but Many Different Transcripts: A Journey into the World of Long Non-Coding RNAsEnrico Alessio0Raphael Severino Bonadio1Lisa Buson2Francesco Chemello3Stefano Cagnin4Department of Biology, University of Padova, 35131 Padova, ItalyDepartment of Biology, University of Padova, 35131 Padova, ItalyDepartment of Biology, University of Padova, 35131 Padova, ItalyDepartment of Biology, University of Padova, 35131 Padova, ItalyDepartment of Biology, University of Padova, 35131 Padova, ItalyIn late 2012 it was evidenced that most of the human genome is transcribed but only a small percentage of the transcripts are translated. This observation supported the importance of non-coding RNAs and it was confirmed in several organisms. The most abundant non-translated transcripts are long non-coding RNAs (lncRNAs). In contrast to protein-coding RNAs, they show a more cell-specific expression. To understand the function of lncRNAs, it is fundamental to investigate in which cells they are preferentially expressed and to detect their subcellular localization. Recent improvements of techniques that localize single RNA molecules in tissues like single-cell RNA sequencing and fluorescence amplification methods have given a considerable boost in the knowledge of the lncRNA functions. In recent years, single-cell transcription variability was associated with non-coding RNA expression, revealing this class of RNAs as important transcripts in the cell lineage specification. The purpose of this review is to collect updated information about lncRNA classification and new findings on their function derived from single-cell analysis. We also retained useful for all researchers to describe the methods available for single-cell analysis and the databases collecting single-cell and lncRNA data. Tables are included to schematize, describe, and compare exposed concepts.https://www.mdpi.com/1422-0067/21/1/302single-cellsingle-cell sequencingsingle-cell expressionnon-coding rnaslong non-coding rnaslncrnaslncrna databasesingle-cell database |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Enrico Alessio Raphael Severino Bonadio Lisa Buson Francesco Chemello Stefano Cagnin |
spellingShingle |
Enrico Alessio Raphael Severino Bonadio Lisa Buson Francesco Chemello Stefano Cagnin A Single Cell but Many Different Transcripts: A Journey into the World of Long Non-Coding RNAs International Journal of Molecular Sciences single-cell single-cell sequencing single-cell expression non-coding rnas long non-coding rnas lncrnas lncrna database single-cell database |
author_facet |
Enrico Alessio Raphael Severino Bonadio Lisa Buson Francesco Chemello Stefano Cagnin |
author_sort |
Enrico Alessio |
title |
A Single Cell but Many Different Transcripts: A Journey into the World of Long Non-Coding RNAs |
title_short |
A Single Cell but Many Different Transcripts: A Journey into the World of Long Non-Coding RNAs |
title_full |
A Single Cell but Many Different Transcripts: A Journey into the World of Long Non-Coding RNAs |
title_fullStr |
A Single Cell but Many Different Transcripts: A Journey into the World of Long Non-Coding RNAs |
title_full_unstemmed |
A Single Cell but Many Different Transcripts: A Journey into the World of Long Non-Coding RNAs |
title_sort |
single cell but many different transcripts: a journey into the world of long non-coding rnas |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-01-01 |
description |
In late 2012 it was evidenced that most of the human genome is transcribed but only a small percentage of the transcripts are translated. This observation supported the importance of non-coding RNAs and it was confirmed in several organisms. The most abundant non-translated transcripts are long non-coding RNAs (lncRNAs). In contrast to protein-coding RNAs, they show a more cell-specific expression. To understand the function of lncRNAs, it is fundamental to investigate in which cells they are preferentially expressed and to detect their subcellular localization. Recent improvements of techniques that localize single RNA molecules in tissues like single-cell RNA sequencing and fluorescence amplification methods have given a considerable boost in the knowledge of the lncRNA functions. In recent years, single-cell transcription variability was associated with non-coding RNA expression, revealing this class of RNAs as important transcripts in the cell lineage specification. The purpose of this review is to collect updated information about lncRNA classification and new findings on their function derived from single-cell analysis. We also retained useful for all researchers to describe the methods available for single-cell analysis and the databases collecting single-cell and lncRNA data. Tables are included to schematize, describe, and compare exposed concepts. |
topic |
single-cell single-cell sequencing single-cell expression non-coding rnas long non-coding rnas lncrnas lncrna database single-cell database |
url |
https://www.mdpi.com/1422-0067/21/1/302 |
work_keys_str_mv |
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