Investigation of SLC6A4 gene expression in autism spectrum disorders
Objective: Autism is defined as a complex neurodevelopmental disorder. Genetics plays a major role in the etiology of autism spectrum disorders (ASD). The role of the serotonin in the development of autism has been widely investigated. SLC6A4 gene (SERT or 5-HT) has an important role reuptaking of s...
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2015-06-01
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doaj-fa229225d30446068646dd922ae2ce562021-09-02T06:56:28ZengModestum Publishing LTDJournal of Clinical and Experimental Investigations1309-85781309-66212015-06-016216516910.5799/ahinjs.01.2015.02.0510Investigation of SLC6A4 gene expression in autism spectrum disordersElif Funda Şener0Keziban Korkmaz1Didem Behice Öztop2Gökmen Zararsız3Yusuf Özkul4 Erciyes Üniversitesi Tıp Fakültesi, Tıbbi Biyoloji AD, Kayseri, TürkiyeErciyes Üniversitesi Genom ve Kök Hücre Merkezi (GENKÖK), Kayseri, TürkiyeErciyes Üniversitesi Tıp Fakültesi, Çocuk Psikiyatri AD, Kayseri, TürkiyeErciyes Üniversitesi Tıp Fakültesi, Biyoistatistik AD, Kayseri, TürkiyeErciyes Üniversitesi Tıp Fakültesi, Tıbbi Genetik AD, Kayseri, TürkiyeObjective: Autism is defined as a complex neurodevelopmental disorder. Genetics plays a major role in the etiology of autism spectrum disorders (ASD). The role of the serotonin in the development of autism has been widely investigated. SLC6A4 gene (SERT or 5-HT) has an important role reuptaking of serotonin. Because of this, our study examined the expression level of SLC6A4 gene in autism patients. Methods: Thirty-four patients (26 male, 8 female) who diagnosed as autism firstly according to DSM-V criteria in the Department of child psychiatry, Erciyes University Medical Faculty and healthy 23 controls (16 male, 7 female) were enrolled in this study. Total RNA was isolated from peripheral blood samples using TRIzol. Quantitative Real-time PCR (qRT-PCR) was performed to detect SLC6A4 gene expression. Results: SLC6A4 gene expression was found statistically significant and low in autism group compared with controls (p=0,027). Conclusion: The low gene expression in the patient group implied that there is an abnormality of serotonin reuptake. According to our results, we suggest that much more studies may be planned with the expression and methylation profile of this gene combined with gene polymorphisms especially affecting the expression in larger sample sizes. J Clin Exp Invest 2015; 6 (2): 165-169http://www.jceionline.org/upload/sayi/27/JCEI-01171.pdfAutismExpressionGeneSerotonin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elif Funda Şener Keziban Korkmaz Didem Behice Öztop Gökmen Zararsız Yusuf Özkul |
spellingShingle |
Elif Funda Şener Keziban Korkmaz Didem Behice Öztop Gökmen Zararsız Yusuf Özkul Investigation of SLC6A4 gene expression in autism spectrum disorders Journal of Clinical and Experimental Investigations Autism Expression Gene Serotonin |
author_facet |
Elif Funda Şener Keziban Korkmaz Didem Behice Öztop Gökmen Zararsız Yusuf Özkul |
author_sort |
Elif Funda Şener |
title |
Investigation of SLC6A4 gene expression in autism spectrum disorders |
title_short |
Investigation of SLC6A4 gene expression in autism spectrum disorders |
title_full |
Investigation of SLC6A4 gene expression in autism spectrum disorders |
title_fullStr |
Investigation of SLC6A4 gene expression in autism spectrum disorders |
title_full_unstemmed |
Investigation of SLC6A4 gene expression in autism spectrum disorders |
title_sort |
investigation of slc6a4 gene expression in autism spectrum disorders |
publisher |
Modestum Publishing LTD |
series |
Journal of Clinical and Experimental Investigations |
issn |
1309-8578 1309-6621 |
publishDate |
2015-06-01 |
description |
Objective: Autism is defined as a complex neurodevelopmental disorder. Genetics plays a major role in the etiology of autism spectrum disorders (ASD). The role of the serotonin in the development of autism has been widely investigated. SLC6A4 gene (SERT or 5-HT) has an important role reuptaking of serotonin. Because of this, our study examined the expression level of SLC6A4 gene in autism patients.
Methods: Thirty-four patients (26 male, 8 female) who diagnosed as autism firstly according to DSM-V criteria in the Department of child psychiatry, Erciyes University Medical Faculty and healthy 23 controls (16 male, 7 female) were enrolled in this study. Total RNA was isolated from peripheral blood samples using TRIzol. Quantitative Real-time PCR (qRT-PCR) was performed to detect SLC6A4 gene expression.
Results: SLC6A4 gene expression was found statistically significant and low in autism group compared with controls (p=0,027).
Conclusion: The low gene expression in the patient group implied that there is an abnormality of serotonin reuptake. According to our results, we suggest that much more studies may be planned with the expression and methylation profile of this gene combined with gene polymorphisms especially affecting the expression in larger sample sizes. J Clin Exp Invest 2015; 6 (2): 165-169 |
topic |
Autism Expression Gene Serotonin |
url |
http://www.jceionline.org/upload/sayi/27/JCEI-01171.pdf |
work_keys_str_mv |
AT eliffundasener investigationofslc6a4geneexpressioninautismspectrumdisorders AT kezibankorkmaz investigationofslc6a4geneexpressioninautismspectrumdisorders AT didembehiceoztop investigationofslc6a4geneexpressioninautismspectrumdisorders AT gokmenzararsız investigationofslc6a4geneexpressioninautismspectrumdisorders AT yusufozkul investigationofslc6a4geneexpressioninautismspectrumdisorders |
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