Successful Reversal of Diabetes by Single Donor Isologous Islet Transplantation in a Mouse Model
A method for isolating mouse islets which consistently gives a high yield with good purity is described. Using a bovine serum albumin gradient, the mean yield of islets per pancreas is 425 (SEM ± 15) with a consistent purity of over 90%. Single donor to single recipient of islets transplanted under...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
1997-07-01
|
| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/096368979700600410 |
| id |
doaj-fa288fdab9a24cafa8538940cb19952e |
|---|---|
| record_format |
Article |
| spelling |
doaj-fa288fdab9a24cafa8538940cb19952e2020-11-25T03:03:21ZengSAGE PublishingCell Transplantation0963-68971555-38921997-07-01610.1177/096368979700600410Successful Reversal of Diabetes by Single Donor Isologous Islet Transplantation in a Mouse ModelC. K. Leow0D. W. R. Gray1P. J. Morris2Nuffield Department of Surgery, John Radcliffe Hospital, Headington, Oxford, UKNuffield Department of Surgery, John Radcliffe Hospital, Headington, Oxford, UKNuffield Department of Surgery, John Radcliffe Hospital, Headington, Oxford, UKA method for isolating mouse islets which consistently gives a high yield with good purity is described. Using a bovine serum albumin gradient, the mean yield of islets per pancreas is 425 (SEM ± 15) with a consistent purity of over 90%. Single donor to single recipient of islets transplanted under the renal capsule restores normoglycemia in the diabetic recipients within 2 to 5 days of transplantation.https://doi.org/10.1177/096368979700600410 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
C. K. Leow D. W. R. Gray P. J. Morris |
| spellingShingle |
C. K. Leow D. W. R. Gray P. J. Morris Successful Reversal of Diabetes by Single Donor Isologous Islet Transplantation in a Mouse Model Cell Transplantation |
| author_facet |
C. K. Leow D. W. R. Gray P. J. Morris |
| author_sort |
C. K. Leow |
| title |
Successful Reversal of Diabetes by Single Donor Isologous Islet Transplantation in a Mouse Model |
| title_short |
Successful Reversal of Diabetes by Single Donor Isologous Islet Transplantation in a Mouse Model |
| title_full |
Successful Reversal of Diabetes by Single Donor Isologous Islet Transplantation in a Mouse Model |
| title_fullStr |
Successful Reversal of Diabetes by Single Donor Isologous Islet Transplantation in a Mouse Model |
| title_full_unstemmed |
Successful Reversal of Diabetes by Single Donor Isologous Islet Transplantation in a Mouse Model |
| title_sort |
successful reversal of diabetes by single donor isologous islet transplantation in a mouse model |
| publisher |
SAGE Publishing |
| series |
Cell Transplantation |
| issn |
0963-6897 1555-3892 |
| publishDate |
1997-07-01 |
| description |
A method for isolating mouse islets which consistently gives a high yield with good purity is described. Using a bovine serum albumin gradient, the mean yield of islets per pancreas is 425 (SEM ± 15) with a consistent purity of over 90%. Single donor to single recipient of islets transplanted under the renal capsule restores normoglycemia in the diabetic recipients within 2 to 5 days of transplantation. |
| url |
https://doi.org/10.1177/096368979700600410 |
| work_keys_str_mv |
AT ckleow successfulreversalofdiabetesbysingledonorisologousislettransplantationinamousemodel AT dwrgray successfulreversalofdiabetesbysingledonorisologousislettransplantationinamousemodel AT pjmorris successfulreversalofdiabetesbysingledonorisologousislettransplantationinamousemodel |
| _version_ |
1724686253399474176 |