High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics

Lemos Duarte et al. describe a strategy to produce, validate, and utilize highly-specific recombinant antibodies. As a proof of principle, they demonstrate development and validation of a panel of antibodies against proteins relevant to signaling by opiates. Their antibody-based tool can be useful i...

Full description

Bibliographic Details
Main Authors:	Mariana Lemos Duarte, Nikita A. Trimbake, Achla Gupta, Christine Tumanut, Xiaomin Fan, Catherine Woods, Akila Ram, Ivone Gomes, Erin N. Bobeck, Deborah Schechtman, Lakshmi A. Devi
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-02-01
Series:	Communications Biology
Online Access:	https://doi.org/10.1038/s42003-021-01744-8

id	doaj-fa3462494d824628b60f24925ecc50e1
record_format	Article
spelling	doaj-fa3462494d824628b60f24925ecc50e12021-02-23T09:14:59ZengNature Publishing GroupCommunications Biology2399-36422021-02-014111310.1038/s42003-021-01744-8High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamicsMariana Lemos Duarte0Nikita A. Trimbake1Achla Gupta2Christine Tumanut3Xiaomin Fan4Catherine Woods5Akila Ram6Ivone Gomes7Erin N. Bobeck8Deborah Schechtman9Lakshmi A. Devi10Department of Pharmacological Sciences, Icahn School of Medicine at Mount SinaiDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount SinaiDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount SinaiAvantGen Inc.AvantGen Inc.AvantGen Inc.Department of Biology, Utah State UniversityDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount SinaiDepartment of Biology, Utah State UniversityDepartment of Biochemistry, University of São PauloDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount SinaiLemos Duarte et al. describe a strategy to produce, validate, and utilize highly-specific recombinant antibodies. As a proof of principle, they demonstrate development and validation of a panel of antibodies against proteins relevant to signaling by opiates. Their antibody-based tool can be useful in understanding the spatio-temporal dynamics of opioid signaling.https://doi.org/10.1038/s42003-021-01744-8
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Mariana Lemos Duarte Nikita A. Trimbake Achla Gupta Christine Tumanut Xiaomin Fan Catherine Woods Akila Ram Ivone Gomes Erin N. Bobeck Deborah Schechtman Lakshmi A. Devi
spellingShingle	Mariana Lemos Duarte Nikita A. Trimbake Achla Gupta Christine Tumanut Xiaomin Fan Catherine Woods Akila Ram Ivone Gomes Erin N. Bobeck Deborah Schechtman Lakshmi A. Devi High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics Communications Biology
author_facet	Mariana Lemos Duarte Nikita A. Trimbake Achla Gupta Christine Tumanut Xiaomin Fan Catherine Woods Akila Ram Ivone Gomes Erin N. Bobeck Deborah Schechtman Lakshmi A. Devi
author_sort	Mariana Lemos Duarte
title	High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics
title_short	High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics
title_full	High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics
title_fullStr	High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics
title_full_unstemmed	High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics
title_sort	high-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics
publisher	Nature Publishing Group
series	Communications Biology
issn	2399-3642
publishDate	2021-02-01
description	Lemos Duarte et al. describe a strategy to produce, validate, and utilize highly-specific recombinant antibodies. As a proof of principle, they demonstrate development and validation of a panel of antibodies against proteins relevant to signaling by opiates. Their antibody-based tool can be useful in understanding the spatio-temporal dynamics of opioid signaling.
url	https://doi.org/10.1038/s42003-021-01744-8
work_keys_str_mv	AT marianalemosduarte highthroughputscreeningandvalidationofantibodiesagainstsynapticproteinstoexploreopioidsignalingdynamics AT nikitaatrimbake highthroughputscreeningandvalidationofantibodiesagainstsynapticproteinstoexploreopioidsignalingdynamics AT achlagupta highthroughputscreeningandvalidationofantibodiesagainstsynapticproteinstoexploreopioidsignalingdynamics AT christinetumanut highthroughputscreeningandvalidationofantibodiesagainstsynapticproteinstoexploreopioidsignalingdynamics AT xiaominfan highthroughputscreeningandvalidationofantibodiesagainstsynapticproteinstoexploreopioidsignalingdynamics AT catherinewoods highthroughputscreeningandvalidationofantibodiesagainstsynapticproteinstoexploreopioidsignalingdynamics AT akilaram highthroughputscreeningandvalidationofantibodiesagainstsynapticproteinstoexploreopioidsignalingdynamics AT ivonegomes highthroughputscreeningandvalidationofantibodiesagainstsynapticproteinstoexploreopioidsignalingdynamics AT erinnbobeck highthroughputscreeningandvalidationofantibodiesagainstsynapticproteinstoexploreopioidsignalingdynamics AT deborahschechtman highthroughputscreeningandvalidationofantibodiesagainstsynapticproteinstoexploreopioidsignalingdynamics AT lakshmiadevi highthroughputscreeningandvalidationofantibodiesagainstsynapticproteinstoexploreopioidsignalingdynamics
_version_	1724254955228889088

High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics

Similar Items