High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics
Lemos Duarte et al. describe a strategy to produce, validate, and utilize highly-specific recombinant antibodies. As a proof of principle, they demonstrate development and validation of a panel of antibodies against proteins relevant to signaling by opiates. Their antibody-based tool can be useful i...
Main Authors: | Mariana Lemos Duarte, Nikita A. Trimbake, Achla Gupta, Christine Tumanut, Xiaomin Fan, Catherine Woods, Akila Ram, Ivone Gomes, Erin N. Bobeck, Deborah Schechtman, Lakshmi A. Devi |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2021-02-01
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Series: | Communications Biology |
Online Access: | https://doi.org/10.1038/s42003-021-01744-8 |
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