Correlation between Peripheral Blood T-cell Profiles and Clinical and Inflammatory Parameters in Stable COPD
Background: Recent studies suggest that Tcl/Tc2 imbalances are implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD). The purpose of this study was to clarify the relationship between peripheral blood T-cell profiles and pulmonary function or inflammatory parameters. Methods...
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doaj-fa45619531744c4caf6274749c9ba99c2020-11-24T23:53:40ZengElsevierAllergology International1323-89302010-01-01591758210.2332/allergolint.09-OA-0126Correlation between Peripheral Blood T-cell Profiles and Clinical and Inflammatory Parameters in Stable COPDToshihiro Shirai0Takafumi Suda1Naoki Inui2Kingo Chida3Department of Respiratory Medicine, Shizuoka General Hospital, Hamamatsu University School of Medicine, Shizuoka, JapanDepartment of Respiratory Medicine, Hamamatsu University School of Medicine, Shizuoka, Japan.Department of Respiratory Medicine, Hamamatsu University School of Medicine, Shizuoka, Japan.Department of Respiratory Medicine, Hamamatsu University School of Medicine, Shizuoka, Japan.Background: Recent studies suggest that Tcl/Tc2 imbalances are implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD). The purpose of this study was to clarify the relationship between peripheral blood T-cell profiles and pulmonary function or inflammatory parameters. Methods: Thirty-one patients with stable COPD (median age 70 years, 30 males, 15 current smokers and 16 ex-smokers) and 30 healthy control subjects were enrolled in this study. The subjects underwent blood tests, exhaled nitric oxide (eNO) measurement, pulmonary function tests, and sputum induction. Tc1/Tc2 and Th1/Th2 were determined by analyzing intracellular cytokine staining for IFN-γ and IL-4 in peripheral blood CD8+ and CD4+ T cells using flow cytometry after stimulation with phorbol 12-myristate 13-acetate and ionomycin. Results: There was a significantly increased proportion of IFN-γ-producing and IL-4-producing CD8+ T cells in patients with COPD compared with control subjects (median [IQR] 73.6% [63.9%-80.7%] vs 62.0% [45.6%-73.8%], p = 0.004; and 2.6% [1.1%-6.9%] vs 1.1% [0.6%-2.2%], p = 0.002, respectively). In addition, the proportion of IFN-γ-producing CD4+ T cells was significantly higher in patients with COPD compared with control subjects (25.7% [21.2%-38.0%] vs 22.8% [15.6%-29.2%], p = 0.027). The proportion of IFN-γ-producing CD8+ T cells was correlated negatively with single-breath carbon monoxide transfer coefficient (Kco) (ρ = −0.45, p = 0.033) and positively with eNO (ρ = 0.50, p = 0.012). The proportion of IL-4-producing CD8+ T cells was positively correlated with body mass index (ρ = 0.42, p = 0.023) and Kco (ρ = 0.47, p = 0.026). Conclusions: It is suggested that Tc1 cells have a detrimental role and that Tc2 cells have a protective role in disease progression.http://www.sciencedirect.com/science/article/pii/S1323893015305785chronic obstructive pulmonary diseasecorrelationexhaled nitric oxideTc1/Tc2transfer coefficient |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Toshihiro Shirai Takafumi Suda Naoki Inui Kingo Chida |
spellingShingle |
Toshihiro Shirai Takafumi Suda Naoki Inui Kingo Chida Correlation between Peripheral Blood T-cell Profiles and Clinical and Inflammatory Parameters in Stable COPD Allergology International chronic obstructive pulmonary disease correlation exhaled nitric oxide Tc1/Tc2 transfer coefficient |
author_facet |
Toshihiro Shirai Takafumi Suda Naoki Inui Kingo Chida |
author_sort |
Toshihiro Shirai |
title |
Correlation between Peripheral Blood T-cell Profiles and Clinical and Inflammatory Parameters in Stable COPD |
title_short |
Correlation between Peripheral Blood T-cell Profiles and Clinical and Inflammatory Parameters in Stable COPD |
title_full |
Correlation between Peripheral Blood T-cell Profiles and Clinical and Inflammatory Parameters in Stable COPD |
title_fullStr |
Correlation between Peripheral Blood T-cell Profiles and Clinical and Inflammatory Parameters in Stable COPD |
title_full_unstemmed |
Correlation between Peripheral Blood T-cell Profiles and Clinical and Inflammatory Parameters in Stable COPD |
title_sort |
correlation between peripheral blood t-cell profiles and clinical and inflammatory parameters in stable copd |
publisher |
Elsevier |
series |
Allergology International |
issn |
1323-8930 |
publishDate |
2010-01-01 |
description |
Background: Recent studies suggest that Tcl/Tc2 imbalances are implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD). The purpose of this study was to clarify the relationship between peripheral blood T-cell profiles and pulmonary function or inflammatory parameters.
Methods: Thirty-one patients with stable COPD (median age 70 years, 30 males, 15 current smokers and 16 ex-smokers) and 30 healthy control subjects were enrolled in this study. The subjects underwent blood tests, exhaled nitric oxide (eNO) measurement, pulmonary function tests, and sputum induction. Tc1/Tc2 and Th1/Th2 were determined by analyzing intracellular cytokine staining for IFN-γ and IL-4 in peripheral blood CD8+ and CD4+ T cells using flow cytometry after stimulation with phorbol 12-myristate 13-acetate and ionomycin.
Results: There was a significantly increased proportion of IFN-γ-producing and IL-4-producing CD8+ T cells in patients with COPD compared with control subjects (median [IQR] 73.6% [63.9%-80.7%] vs 62.0% [45.6%-73.8%], p = 0.004; and 2.6% [1.1%-6.9%] vs 1.1% [0.6%-2.2%], p = 0.002, respectively). In addition, the proportion of IFN-γ-producing CD4+ T cells was significantly higher in patients with COPD compared with control subjects (25.7% [21.2%-38.0%] vs 22.8% [15.6%-29.2%], p = 0.027). The proportion of IFN-γ-producing CD8+ T cells was correlated negatively with single-breath carbon monoxide transfer coefficient (Kco) (ρ = −0.45, p = 0.033) and positively with eNO (ρ = 0.50, p = 0.012). The proportion of IL-4-producing CD8+ T cells was positively correlated with body mass index (ρ = 0.42, p = 0.023) and Kco (ρ = 0.47, p = 0.026).
Conclusions: It is suggested that Tc1 cells have a detrimental role and that Tc2 cells have a protective role in disease progression. |
topic |
chronic obstructive pulmonary disease correlation exhaled nitric oxide Tc1/Tc2 transfer coefficient |
url |
http://www.sciencedirect.com/science/article/pii/S1323893015305785 |
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