A comparative study of antibacterial effects of mouthwashes containing Ag/ZnO or ZnO nanoparticles with chlorhexidine and investigation of their cytotoxicity
Objective(s): Chlorhexidine 0.2% mouthwash is commonly used in orthodontic patients for plaque control. But it has some side effects. Metal oxide nanoparticles have been recently used in mouthwashes in reports. So we aimed to evaluate antibacterial effect of ZnO and Ag/ZnO nanoparticles against Stre...
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Mashhad University of Medical Sciences
2018-04-01
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doaj-fa51846cb647439b938ccf999cb130b92020-11-24T23:53:40ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042018-04-015210211010.22038/nmj.2018.005.00710447A comparative study of antibacterial effects of mouthwashes containing Ag/ZnO or ZnO nanoparticles with chlorhexidine and investigation of their cytotoxicitymojgan Kachoei0baharak divband1fatemeh Dabaghi Tabriz2Zahra Norouzzadeh Helali3Mahdieh Esmailzadeh4Department of Orthodontics, Faculty of Dentistry, Tabriz University of Medical SciencesStem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran |Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, IranOperative Dentistry Department, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Orthodontics, Faculty of Dentistry, Tabriz University of Medical SciencesDentistry student ,Faculty of Dentistry , Tabriz University of Medical Sciences, Tabriz ,IranObjective(s): Chlorhexidine 0.2% mouthwash is commonly used in orthodontic patients for plaque control. But it has some side effects. Metal oxide nanoparticles have been recently used in mouthwashes in reports. So we aimed to evaluate antibacterial effect of ZnO and Ag/ZnO nanoparticles against Streptococcus mutans and compare them with chlorhexidine 0.2%, sodium fluoride 0.05% and some of their compositions. Materials and Methods: ZnO and Ag/ZnO NPs were synthesized and sixteen groups of mouthwashes were prepared. We used Zone of Inhibition (ZOI) test to evaluation of antibacterial effects of as-prepared mouthwashes, against S. mutans. The cytotoxicity of the ZnO and Ag/ZnO NPs were investigated in the A549 cell line.Results: Among the study groups, the maximum ZOI (16.60±0.49 mm) pertained to Ag/ZnO, 10 mg NPs plus 100 ml base material, (Ag/ZnO b 10). The results indicate that no significant harmful effect is imposed to the cells up to 0.2 mg/ml of ZnO and Ag/ZnO NPs.Conclusion: Results showed that mouthwash containing Ag/ZnO b 10 has the highest antibacterial properties against S .mutans amoung study groups and because in this concentration it is safe for cells, so it can be served as an alternative mouthwash in plaque control instead of chlorhexidine 0.2% after in vivo studies.http://nmj.mums.ac.ir/article_10447_306cd446256ed4d090ad72efd7203d8a.pdfAg/ZnO nanoparticleAntibacterialMTT AssayMouthwashZnO nanoparticle |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
mojgan Kachoei baharak divband fatemeh Dabaghi Tabriz Zahra Norouzzadeh Helali Mahdieh Esmailzadeh |
spellingShingle |
mojgan Kachoei baharak divband fatemeh Dabaghi Tabriz Zahra Norouzzadeh Helali Mahdieh Esmailzadeh A comparative study of antibacterial effects of mouthwashes containing Ag/ZnO or ZnO nanoparticles with chlorhexidine and investigation of their cytotoxicity Nanomedicine Journal Ag/ZnO nanoparticle Antibacterial MTT Assay Mouthwash ZnO nanoparticle |
author_facet |
mojgan Kachoei baharak divband fatemeh Dabaghi Tabriz Zahra Norouzzadeh Helali Mahdieh Esmailzadeh |
author_sort |
mojgan Kachoei |
title |
A comparative study of antibacterial effects of mouthwashes containing Ag/ZnO or ZnO nanoparticles with chlorhexidine and investigation of their cytotoxicity |
title_short |
A comparative study of antibacterial effects of mouthwashes containing Ag/ZnO or ZnO nanoparticles with chlorhexidine and investigation of their cytotoxicity |
title_full |
A comparative study of antibacterial effects of mouthwashes containing Ag/ZnO or ZnO nanoparticles with chlorhexidine and investigation of their cytotoxicity |
title_fullStr |
A comparative study of antibacterial effects of mouthwashes containing Ag/ZnO or ZnO nanoparticles with chlorhexidine and investigation of their cytotoxicity |
title_full_unstemmed |
A comparative study of antibacterial effects of mouthwashes containing Ag/ZnO or ZnO nanoparticles with chlorhexidine and investigation of their cytotoxicity |
title_sort |
comparative study of antibacterial effects of mouthwashes containing ag/zno or zno nanoparticles with chlorhexidine and investigation of their cytotoxicity |
publisher |
Mashhad University of Medical Sciences |
series |
Nanomedicine Journal |
issn |
2322-3049 2322-5904 |
publishDate |
2018-04-01 |
description |
Objective(s): Chlorhexidine 0.2% mouthwash is commonly used in orthodontic patients for plaque control. But it has some side effects. Metal oxide nanoparticles have been recently used in mouthwashes in reports. So we aimed to evaluate antibacterial effect of ZnO and Ag/ZnO nanoparticles against Streptococcus mutans and compare them with chlorhexidine 0.2%, sodium fluoride 0.05% and some of their compositions. Materials and Methods: ZnO and Ag/ZnO NPs were synthesized and sixteen groups of mouthwashes were prepared. We used Zone of Inhibition (ZOI) test to evaluation of antibacterial effects of as-prepared mouthwashes, against S. mutans. The cytotoxicity of the ZnO and Ag/ZnO NPs were investigated in the A549 cell line.Results: Among the study groups, the maximum ZOI (16.60±0.49 mm) pertained to Ag/ZnO, 10 mg NPs plus 100 ml base material, (Ag/ZnO b 10). The results indicate that no significant harmful effect is imposed to the cells up to 0.2 mg/ml of ZnO and Ag/ZnO NPs.Conclusion: Results showed that mouthwash containing Ag/ZnO b 10 has the highest antibacterial properties against S .mutans amoung study groups and because in this concentration it is safe for cells, so it can be served as an alternative mouthwash in plaque control instead of chlorhexidine 0.2% after in vivo studies. |
topic |
Ag/ZnO nanoparticle Antibacterial MTT Assay Mouthwash ZnO nanoparticle |
url |
http://nmj.mums.ac.ir/article_10447_306cd446256ed4d090ad72efd7203d8a.pdf |
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