Salmonella contamination of eggs of native Kohgiluyeh va Boyerahmad using PCR1 techniques and the evaluation of drug resistance

• Main Authors: M Monadi, M Kargar, A Naghiha, R Mohammadi
• Format: Article
• Language: fas
• Published: Yasuj University Of Medical Sciences 2014-05-01
• Series: Armaghane Danesh Bimonthly Journal
• Subjects: Key words: Salmonella; Egg; drug resistance; invA; PCR.
• Online Access: http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-309&slc_lang=en&sid=1

Abstract Background & aim:Foodborne disease, a major health and economic problem in industrialized and non-industrialized countries.The purpose of this study was to investigate Salmonella contamination of eggs by native province kohgiloyeh va Boyerahmad by PCR and evaluation of their drug resistance. Methods: This cross-sectional study-descriptive study of 210 eggs collected from native Kohgiluyeh va Boyerahmad done. Biochemical tests for identification of bacteria was isolated. Salmonella bacteria have suspected reactions were tested by PCR with specific primers invA genes were examined. Results: The results showed that 14 number of eggs (6/66 percent) were contaminated with Salmonella genus. Dehdasht area of highest contamination and less pollution Charusa areas, Dyshmuk, Lndeh and was Basht And no significant correlation was found between the type and extent of contamination and the region.The antibiotic resistance of most resistance to penicillin (100%) was observed.This study uses data from the nineteenth and application soft ware spss version microsofte office 2007-square test and Fisher were analyzed. Significant level of p>0/05 was considered. Conclusion: Microbial agents such as Salmonella can cause food spoilage and disease are. Resistance in Salmonellais recommended to avoid the in discriminate use of antibiotics in live stock and poultry should be avoided. Key words: Salmonella,Egg,drug resistance, invA, PCR.