Effect of bacteria used in food industry on the proliferation and cytokine production of epithelial intestinal cellular lines

Effect of bacteria used in food industry on the proliferation and cytokine production of epithelial intestinal cellular lines

The gastrointestinal mucosa, composed mainly of a monolayer of intestinal epithelial cells (IEC) covered by mucus, represents the first contact point of gut and orally ingested bacteria with the host. This bacteria-IEC interaction is important for maintenance of gut homeostasis. Here we assessed the...

Full description

Bibliographic Details
Main Authors: Claudio Hidalgo-Cantabrana, Riina Kekkonen, Clara G. de los Reyes-Gavilán, Seppo Salminen, Riitta Korpela, Miguel Gueimonde, Patricia Ruas-Madiedo
Format: Article
Language:English
Published: Elsevier 2014-01-01
Series:Journal of Functional Foods
Subjects:
Probiotic
Food bacteria
Cellular lines
Cytokines
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464613002545
id doaj-fa6eb49262d2419d80a79776a29feeff
record_format Article
spelling doaj-fa6eb49262d2419d80a79776a29feeff2021-04-29T04:41:49ZengElsevierJournal of Functional Foods1756-46462014-01-016348355Effect of bacteria used in food industry on the proliferation and cytokine production of epithelial intestinal cellular linesClaudio Hidalgo-Cantabrana0Riina Kekkonen1Clara G. de los Reyes-Gavilán2Seppo Salminen3Riitta Korpela4Miguel Gueimonde5Patricia Ruas-Madiedo6Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares, s/n, 33300 Villaviciosa, Asturias, SpainValio Research Centre, Meijeritie 4, 00370 Helsinki, FinlandInstituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares, s/n, 33300 Villaviciosa, Asturias, SpainFunctional Food Forum, Itäinen Pitkäkatu 4A, 20014 Turku, FinlandInstitute of Biomedicine, Pharmacology, Faculty of Medicine, University of Helsinki, Haartmaninkatu 8, 00014 Helsinki, FinlandInstituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares, s/n, 33300 Villaviciosa, Asturias, SpainInstituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares, s/n, 33300 Villaviciosa, Asturias, Spain; Corresponding author. Tel.: +34 985 892131; fax: +34 985 892233.The gastrointestinal mucosa, composed mainly of a monolayer of intestinal epithelial cells (IEC) covered by mucus, represents the first contact point of gut and orally ingested bacteria with the host. This bacteria-IEC interaction is important for maintenance of gut homeostasis. Here we assessed the capability of eleven bacteria to interact with two IEC types (Caco2 and HT29). The effect of the microorganisms upon IEC growth and proliferation, as well as on cytokine production, was determined. A high dependence on both the IEC line used and the strain tested was observed. None of the strains modified the growth or proliferation of HT29-cells but some affected that of Caco2. The presence of the bacteria did not affect cytokine production by Caco2-cells, but some strains modulated the cytokine production by HT29-cells. IL-1β and IL-6 production was reduced by all bacteria tested and most, except Bifidobacterium longum 1/10, Bifidobacterium breve 99/E8 and Propionibacterium freudenreichii PJS, reduced the production of IL-8. Lactococcus lactis ARH4 was the bacteria stimulating the highest production of TNFα, whilst Pr. freudenreichii PJS significantly increased IL-10 production and Bifidobacterium animalis Bb12 reducing it. This work evidences that the cellular model used to test bacterial host-cells interaction has a great impact on the results obtained.http://www.sciencedirect.com/science/article/pii/S1756464613002545ProbioticFood bacteriaCellular linesCytokines
collection DOAJ
language English
format Article
sources DOAJ
author Claudio Hidalgo-Cantabrana
Riina Kekkonen
Clara G. de los Reyes-Gavilán
Seppo Salminen
Riitta Korpela
Miguel Gueimonde
Patricia Ruas-Madiedo
spellingShingle Claudio Hidalgo-Cantabrana
Riina Kekkonen
Clara G. de los Reyes-Gavilán
Seppo Salminen
Riitta Korpela
Miguel Gueimonde
Patricia Ruas-Madiedo
Effect of bacteria used in food industry on the proliferation and cytokine production of epithelial intestinal cellular lines
Journal of Functional Foods
Probiotic
Food bacteria
Cellular lines
Cytokines
author_facet Claudio Hidalgo-Cantabrana
Riina Kekkonen
Clara G. de los Reyes-Gavilán
Seppo Salminen
Riitta Korpela
Miguel Gueimonde
Patricia Ruas-Madiedo
author_sort Claudio Hidalgo-Cantabrana
title Effect of bacteria used in food industry on the proliferation and cytokine production of epithelial intestinal cellular lines
title_short Effect of bacteria used in food industry on the proliferation and cytokine production of epithelial intestinal cellular lines
title_full Effect of bacteria used in food industry on the proliferation and cytokine production of epithelial intestinal cellular lines
title_fullStr Effect of bacteria used in food industry on the proliferation and cytokine production of epithelial intestinal cellular lines
title_full_unstemmed Effect of bacteria used in food industry on the proliferation and cytokine production of epithelial intestinal cellular lines
title_sort effect of bacteria used in food industry on the proliferation and cytokine production of epithelial intestinal cellular lines
publisher Elsevier
series Journal of Functional Foods
issn 1756-4646
publishDate 2014-01-01
description The gastrointestinal mucosa, composed mainly of a monolayer of intestinal epithelial cells (IEC) covered by mucus, represents the first contact point of gut and orally ingested bacteria with the host. This bacteria-IEC interaction is important for maintenance of gut homeostasis. Here we assessed the capability of eleven bacteria to interact with two IEC types (Caco2 and HT29). The effect of the microorganisms upon IEC growth and proliferation, as well as on cytokine production, was determined. A high dependence on both the IEC line used and the strain tested was observed. None of the strains modified the growth or proliferation of HT29-cells but some affected that of Caco2. The presence of the bacteria did not affect cytokine production by Caco2-cells, but some strains modulated the cytokine production by HT29-cells. IL-1β and IL-6 production was reduced by all bacteria tested and most, except Bifidobacterium longum 1/10, Bifidobacterium breve 99/E8 and Propionibacterium freudenreichii PJS, reduced the production of IL-8. Lactococcus lactis ARH4 was the bacteria stimulating the highest production of TNFα, whilst Pr. freudenreichii PJS significantly increased IL-10 production and Bifidobacterium animalis Bb12 reducing it. This work evidences that the cellular model used to test bacterial host-cells interaction has a great impact on the results obtained.
topic Probiotic
Food bacteria
Cellular lines
Cytokines
url http://www.sciencedirect.com/science/article/pii/S1756464613002545
work_keys_str_mv AT claudiohidalgocantabrana effectofbacteriausedinfoodindustryontheproliferationandcytokineproductionofepithelialintestinalcellularlines
AT riinakekkonen effectofbacteriausedinfoodindustryontheproliferationandcytokineproductionofepithelialintestinalcellularlines
AT claragdelosreyesgavilan effectofbacteriausedinfoodindustryontheproliferationandcytokineproductionofepithelialintestinalcellularlines
AT sepposalminen effectofbacteriausedinfoodindustryontheproliferationandcytokineproductionofepithelialintestinalcellularlines
AT riittakorpela effectofbacteriausedinfoodindustryontheproliferationandcytokineproductionofepithelialintestinalcellularlines
AT miguelgueimonde effectofbacteriausedinfoodindustryontheproliferationandcytokineproductionofepithelialintestinalcellularlines
AT patriciaruasmadiedo effectofbacteriausedinfoodindustryontheproliferationandcytokineproductionofepithelialintestinalcellularlines
_version_ 1721502002834309120

Similar Items