Investigation of Genetic Diversity of Fusarium oxysporum f. sp. fragariae Using PCR-RFLP
Fusarium wilts of strawberry, caused by Fusarium oxysporum f. sp. fragariae, is a serious soil-borne disease. Fusarium wilt causes dramatic yield losses in commercial strawberry production and it is a very stubborn disease to control. Reliable chemical control of strawberry Fusarium wilt disease is...
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doaj-fa7013436bd54965b109350934ee5e0c2020-11-24T23:07:43ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542017-04-0133214014710.5423/PPJ.FT.01.2017.0011PPJ.FT.01.2017.0011Investigation of Genetic Diversity of Fusarium oxysporum f. sp. fragariae Using PCR-RFLPJi-Su Kim0Nam Jun Kang1Youn-Sig Kwak2Choungkeun Lee3Division of Applied Life Science (BK21 Plus) and Institute of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, KoreaDepartment of Agricultural Plant Science and Institute of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, KoreaDivision of Applied Life Science (BK21 Plus) and Institute of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, KoreaR&D Coordination Division, Rural Development Administration, Wanju 55365, KoreaFusarium wilts of strawberry, caused by Fusarium oxysporum f. sp. fragariae, is a serious soil-borne disease. Fusarium wilt causes dramatic yield losses in commercial strawberry production and it is a very stubborn disease to control. Reliable chemical control of strawberry Fusarium wilt disease is not yet available. Moreover, other well-known F. oxysporum have different genetic information from F. oxysporum f. sp. fragariae. This analysis investigates the genetic diversity of strawberry Fusairum wilt pathogen. In total, 110 pathogens were isolated from three major strawberry production regions, namely Sukok, Hadong, Sancheong in Gyeongnam province in South Korea. The isolates were confirmed using F. oxysporum f. sp. fragariae species-specific primer sets. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyses were executed using the internal transcribed spacer, intergenic spacer, translation elongation factor1-α, and β-tubulin genes of the pathogens and four restriction enzymes: AluI, HhaI, HinP1I and HpyCH4V. Regarding results, there were diverse patterns in the three gene regions except for the β-tubulin gene region. Correlation analysis of strawberry cultivation region, cultivation method, variety, and phenotype of isolated pathogen, confirmed that genetic diversity depended on the classification of the cultivated region.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378435/Fusarium wiltPCR-RFLP analysisstrawberry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ji-Su Kim Nam Jun Kang Youn-Sig Kwak Choungkeun Lee |
spellingShingle |
Ji-Su Kim Nam Jun Kang Youn-Sig Kwak Choungkeun Lee Investigation of Genetic Diversity of Fusarium oxysporum f. sp. fragariae Using PCR-RFLP The Plant Pathology Journal Fusarium wilt PCR-RFLP analysis strawberry |
author_facet |
Ji-Su Kim Nam Jun Kang Youn-Sig Kwak Choungkeun Lee |
author_sort |
Ji-Su Kim |
title |
Investigation of Genetic Diversity of Fusarium oxysporum f. sp. fragariae Using PCR-RFLP |
title_short |
Investigation of Genetic Diversity of Fusarium oxysporum f. sp. fragariae Using PCR-RFLP |
title_full |
Investigation of Genetic Diversity of Fusarium oxysporum f. sp. fragariae Using PCR-RFLP |
title_fullStr |
Investigation of Genetic Diversity of Fusarium oxysporum f. sp. fragariae Using PCR-RFLP |
title_full_unstemmed |
Investigation of Genetic Diversity of Fusarium oxysporum f. sp. fragariae Using PCR-RFLP |
title_sort |
investigation of genetic diversity of fusarium oxysporum f. sp. fragariae using pcr-rflp |
publisher |
Hanrimwon Publishing Company |
series |
The Plant Pathology Journal |
issn |
1598-2254 |
publishDate |
2017-04-01 |
description |
Fusarium wilts of strawberry, caused by Fusarium oxysporum f. sp. fragariae, is a serious soil-borne disease. Fusarium wilt causes dramatic yield losses in commercial strawberry production and it is a very stubborn disease to control. Reliable chemical control of strawberry Fusarium wilt disease is not yet available. Moreover, other well-known F. oxysporum have different genetic information from F. oxysporum f. sp. fragariae. This analysis investigates the genetic diversity of strawberry Fusairum wilt pathogen. In total, 110 pathogens were isolated from three major strawberry production regions, namely Sukok, Hadong, Sancheong in Gyeongnam province in South Korea. The isolates were confirmed using F. oxysporum f. sp. fragariae species-specific primer sets. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyses were executed using the internal transcribed spacer, intergenic spacer, translation elongation factor1-α, and β-tubulin genes of the pathogens and four restriction enzymes: AluI, HhaI, HinP1I and HpyCH4V. Regarding results, there were diverse patterns in the three gene regions except for the β-tubulin gene region. Correlation analysis of strawberry cultivation region, cultivation method, variety, and phenotype of isolated pathogen, confirmed that genetic diversity depended on the classification of the cultivated region. |
topic |
Fusarium wilt PCR-RFLP analysis strawberry |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378435/ |
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