Sweepstake evolution revealed by population-genetic analysis of copy-number alterations in single genomes of breast cancer
Single-cell sequencing is a promising technology that can address cancer cell evolution by identifying genetic alterations in individual cells. In a recent study, genome-wide DNA copy numbers of single cells were accurately quantified by single-cell sequencing in breast cancers. Phylogenetic-tree an...
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doaj-fa8ecea2a9a3466b9f940f43c9d4db3f2020-11-25T04:05:19ZengThe Royal SocietyRoyal Society Open Science2054-57032017-01-014910.1098/rsos.171060171060Sweepstake evolution revealed by population-genetic analysis of copy-number alterations in single genomes of breast cancerMamoru KatoDaniel A. VascoRyuichi SuginoDaichi NarushimaAlexander KrasnitzSingle-cell sequencing is a promising technology that can address cancer cell evolution by identifying genetic alterations in individual cells. In a recent study, genome-wide DNA copy numbers of single cells were accurately quantified by single-cell sequencing in breast cancers. Phylogenetic-tree analysis revealed genetically distinct populations, each consisting of homogeneous cells. Bioinformatics methods based on population genetics should be further developed to quantitatively analyse the single-cell sequencing data. We developed a bioinformatics framework that was combined with molecular-evolution theories to analyse copy-number losses. This analysis revealed that most deletions in the breast cancers at the single-cell level were generated by simple stochastic processes. A non-standard type of coalescent theory, the multiple-merger coalescent model, aided by approximate Bayesian computation fit well with the data, allowing us to estimate the population-genetic parameters in addition to false-positive and false-negative rates. The estimated parameters suggest that the cancer cells underwent sweepstake evolution, where only one or very few parental cells produced a descendent cell population. We conclude that breast cancer cells successively substitute in a tumour mass, and the high reproduction of only a portion of cancer cells may confer high adaptability to this cancer.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.171060bioinformaticscancer genomicscopy-number alterationmolecular evolutionsingle-cell sequencingcoalescent theory |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mamoru Kato Daniel A. Vasco Ryuichi Sugino Daichi Narushima Alexander Krasnitz |
spellingShingle |
Mamoru Kato Daniel A. Vasco Ryuichi Sugino Daichi Narushima Alexander Krasnitz Sweepstake evolution revealed by population-genetic analysis of copy-number alterations in single genomes of breast cancer Royal Society Open Science bioinformatics cancer genomics copy-number alteration molecular evolution single-cell sequencing coalescent theory |
author_facet |
Mamoru Kato Daniel A. Vasco Ryuichi Sugino Daichi Narushima Alexander Krasnitz |
author_sort |
Mamoru Kato |
title |
Sweepstake evolution revealed by population-genetic analysis of copy-number alterations in single genomes of breast cancer |
title_short |
Sweepstake evolution revealed by population-genetic analysis of copy-number alterations in single genomes of breast cancer |
title_full |
Sweepstake evolution revealed by population-genetic analysis of copy-number alterations in single genomes of breast cancer |
title_fullStr |
Sweepstake evolution revealed by population-genetic analysis of copy-number alterations in single genomes of breast cancer |
title_full_unstemmed |
Sweepstake evolution revealed by population-genetic analysis of copy-number alterations in single genomes of breast cancer |
title_sort |
sweepstake evolution revealed by population-genetic analysis of copy-number alterations in single genomes of breast cancer |
publisher |
The Royal Society |
series |
Royal Society Open Science |
issn |
2054-5703 |
publishDate |
2017-01-01 |
description |
Single-cell sequencing is a promising technology that can address cancer cell evolution by identifying genetic alterations in individual cells. In a recent study, genome-wide DNA copy numbers of single cells were accurately quantified by single-cell sequencing in breast cancers. Phylogenetic-tree analysis revealed genetically distinct populations, each consisting of homogeneous cells. Bioinformatics methods based on population genetics should be further developed to quantitatively analyse the single-cell sequencing data. We developed a bioinformatics framework that was combined with molecular-evolution theories to analyse copy-number losses. This analysis revealed that most deletions in the breast cancers at the single-cell level were generated by simple stochastic processes. A non-standard type of coalescent theory, the multiple-merger coalescent model, aided by approximate Bayesian computation fit well with the data, allowing us to estimate the population-genetic parameters in addition to false-positive and false-negative rates. The estimated parameters suggest that the cancer cells underwent sweepstake evolution, where only one or very few parental cells produced a descendent cell population. We conclude that breast cancer cells successively substitute in a tumour mass, and the high reproduction of only a portion of cancer cells may confer high adaptability to this cancer. |
topic |
bioinformatics cancer genomics copy-number alteration molecular evolution single-cell sequencing coalescent theory |
url |
https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.171060 |
work_keys_str_mv |
AT mamorukato sweepstakeevolutionrevealedbypopulationgeneticanalysisofcopynumberalterationsinsinglegenomesofbreastcancer AT danielavasco sweepstakeevolutionrevealedbypopulationgeneticanalysisofcopynumberalterationsinsinglegenomesofbreastcancer AT ryuichisugino sweepstakeevolutionrevealedbypopulationgeneticanalysisofcopynumberalterationsinsinglegenomesofbreastcancer AT daichinarushima sweepstakeevolutionrevealedbypopulationgeneticanalysisofcopynumberalterationsinsinglegenomesofbreastcancer AT alexanderkrasnitz sweepstakeevolutionrevealedbypopulationgeneticanalysisofcopynumberalterationsinsinglegenomesofbreastcancer |
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