Clusterization of Knee Joint Hyaline Cartilage of Intact Rats and Rats With Experimental Osteoarthritis
To reveal and study the structural changes of clusters (mosaic) organization of hyaline cartilage in case of osteoarthritis we carried out experiments on its modeling using bilateral transection of anterior cruciate ligaments in 28 knee joints of 14 white rats. The autopsy material at 4, 8 and 12 we...
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Volgograd State University
2014-12-01
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doaj-fa92f9260abb496f81f14a0045e542742020-11-24T20:47:33ZrusVolgograd State UniversityVestnik Volgogradskogo Gosudarstvennogo Universiteta. Seriâ 11. Estestvennye Nauki2306-41532409-27622014-12-01471610.15688/jvolsu11.2014.4.1Clusterization of Knee Joint Hyaline Cartilage of Intact Rats and Rats With Experimental OsteoarthritisNovochadov Valeriy Valeryevich0Krylov Pavel Andreevich1Zaytsev Valeriy Gennadyevich2Volgograd State UniversityVolgograd State UniversityVolgograd State UniversityTo reveal and study the structural changes of clusters (mosaic) organization of hyaline cartilage in case of osteoarthritis we carried out experiments on its modeling using bilateral transection of anterior cruciate ligaments in 28 knee joints of 14 white rats. The autopsy material at 4, 8 and 12 weeks after ligament transaction, and 12 samples from 6 intact rats (control group) completed pool for studying the cartilage morphology. The degree of osteoarthritis progression was evaluated using a semiquantitative scale by Kraus. Quantitative morphology included the estimation of surface factor, cartilage thickness, average chondrocyte density, and matrix optical density in Safranin O staining. The chondrocyte density was calculated to identify microcluster variety in the matrix. The expression of aggrecan and lubricin revealed by immunohistochemical assays made it possible to assess the chondrocyte synthetic ability in the corresponding zones of the cartilage. As a result, we have to conclude that the experimental osteoarthritis is accompanied by changes of cartilage microcluster organization. It becomes more homogeneous with less of dense and lubricin production in the superficial zone. Large clusters with a peculiar hypercellularity and heterogeneity of the density matrix are formed in the deep zone. Structural relations with the underlying bone are disordered, single osteophytes appear. The received data may particularly explain the mechanisms of osteoarthritis progression.http://ns.jvolsu.com/index.php/en/component/attachments/download/264articular cartilagechondrocytesextracellular matriximmune histochemistryosteoarthritis |
collection |
DOAJ |
language |
Russian |
format |
Article |
sources |
DOAJ |
author |
Novochadov Valeriy Valeryevich Krylov Pavel Andreevich Zaytsev Valeriy Gennadyevich |
spellingShingle |
Novochadov Valeriy Valeryevich Krylov Pavel Andreevich Zaytsev Valeriy Gennadyevich Clusterization of Knee Joint Hyaline Cartilage of Intact Rats and Rats With Experimental Osteoarthritis Vestnik Volgogradskogo Gosudarstvennogo Universiteta. Seriâ 11. Estestvennye Nauki articular cartilage chondrocytes extracellular matrix immune histochemistry osteoarthritis |
author_facet |
Novochadov Valeriy Valeryevich Krylov Pavel Andreevich Zaytsev Valeriy Gennadyevich |
author_sort |
Novochadov Valeriy Valeryevich |
title |
Clusterization of Knee Joint Hyaline Cartilage of Intact Rats and Rats With Experimental Osteoarthritis |
title_short |
Clusterization of Knee Joint Hyaline Cartilage of Intact Rats and Rats With Experimental Osteoarthritis |
title_full |
Clusterization of Knee Joint Hyaline Cartilage of Intact Rats and Rats With Experimental Osteoarthritis |
title_fullStr |
Clusterization of Knee Joint Hyaline Cartilage of Intact Rats and Rats With Experimental Osteoarthritis |
title_full_unstemmed |
Clusterization of Knee Joint Hyaline Cartilage of Intact Rats and Rats With Experimental Osteoarthritis |
title_sort |
clusterization of knee joint hyaline cartilage of intact rats and rats with experimental osteoarthritis |
publisher |
Volgograd State University |
series |
Vestnik Volgogradskogo Gosudarstvennogo Universiteta. Seriâ 11. Estestvennye Nauki |
issn |
2306-4153 2409-2762 |
publishDate |
2014-12-01 |
description |
To reveal and study the structural changes of clusters (mosaic) organization of hyaline cartilage in case of osteoarthritis we carried out experiments on its modeling using bilateral transection of anterior cruciate ligaments in 28 knee joints of 14 white rats. The autopsy material at 4, 8 and 12 weeks after ligament transaction, and 12 samples from 6 intact rats (control group) completed pool for studying the cartilage morphology. The degree of osteoarthritis progression was evaluated using a semiquantitative scale by Kraus. Quantitative morphology included the estimation of surface factor, cartilage thickness, average chondrocyte density, and matrix optical density in Safranin O staining. The chondrocyte density was calculated to identify microcluster variety in the matrix. The expression of aggrecan and lubricin revealed by immunohistochemical assays made it possible to assess the chondrocyte synthetic ability in the corresponding zones of the cartilage. As a result, we have to conclude that the experimental osteoarthritis is accompanied by changes of cartilage microcluster organization. It becomes more homogeneous with less of dense and lubricin production in the superficial zone. Large clusters with a peculiar hypercellularity and heterogeneity of the density matrix are formed in the deep zone. Structural relations with the underlying bone are disordered, single osteophytes appear. The received data may particularly explain the mechanisms of osteoarthritis progression. |
topic |
articular cartilage chondrocytes extracellular matrix immune histochemistry osteoarthritis |
url |
http://ns.jvolsu.com/index.php/en/component/attachments/download/264 |
work_keys_str_mv |
AT novochadovvaleriyvaleryevich clusterizationofkneejointhyalinecartilageofintactratsandratswithexperimentalosteoarthritis AT krylovpavelandreevich clusterizationofkneejointhyalinecartilageofintactratsandratswithexperimentalosteoarthritis AT zaytsevvaleriygennadyevich clusterizationofkneejointhyalinecartilageofintactratsandratswithexperimentalosteoarthritis |
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1716809645459243008 |