Molecular Alterations in Spermatozoa of a Family Case Living in the Land of Fires. A First Look at Possible Transgenerational Effects of Pollutants
In our previous work, we reported alterations in protamines/histones ratio, in DNA binding of these proteins and their involvement in DNA oxidative damage in 84% of the young men living in the Land of Fires. In the present work, we extended our findings, evaluating any alterations in spermatozoa of...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-09-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/21/18/6710 |
id |
doaj-fa9b9c451efd4b0c9a3399e3af4881e3 |
---|---|
record_format |
Article |
spelling |
doaj-fa9b9c451efd4b0c9a3399e3af4881e32020-11-25T01:21:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-09-01216710671010.3390/ijms21186710Molecular Alterations in Spermatozoa of a Family Case Living in the Land of Fires. A First Look at Possible Transgenerational Effects of PollutantsGennaro Lettieri0Federica Marra1Claudia Moriello2Marina Prisco3Tiziana Notari4Marco Trifuoggi5Antonella Giarra6Liana Bosco7Luigi Montano8Marina Piscopo9Department of Biology, University of Naples Federico II, 80126 Napoli, ItalyDepartment of Biology, University of Naples Federico II, 80126 Napoli, ItalyDepartment of Biology, University of Naples Federico II, 80126 Napoli, ItalyDepartment of Biology, University of Naples Federico II, 80126 Napoli, ItalyCheck Up—Day Surgery, Polydiagnostic and Research Centre, Reproductive Medicine Unit, 84131 Salerno, ItalyDepartment of Chemical Sciences, University of Naples Federico II, Via Cinthia, 21, 80126 Naples, ItalyDepartment of Chemical Sciences, University of Naples Federico II, Via Cinthia, 21, 80126 Naples, ItalyDepartment of Biological, Chemistry and Pharmaceutical Sciences and Technologies, University of Palermo, Viale delle Scienze Ed.16, 90128 Palermo, ItalyAndrology Unit of the “S. Francesco d’Assisi” Hospital, Local Health Authority (ASL) Salerno, EcoFoodFertility Project Coordination Unit, 84020 Oliveto Citra, ItalyDepartment of Biology, University of Naples Federico II, 80126 Napoli, ItalyIn our previous work, we reported alterations in protamines/histones ratio, in DNA binding of these proteins and their involvement in DNA oxidative damage in 84% of the young men living in the Land of Fires. In the present work, we extended our findings, evaluating any alterations in spermatozoa of a family case, a father and son, living in this area, to also give a first look at the possibility of transgenerational inherited effects of environmental contaminants on the molecular alterations of sperm nuclear basic proteins (SNBP), DNA and semen parameters. In the father and son, we found a diverse excess of copper and chromium in the semen, different alterations in SNBP content and low DNA binding affinity of these proteins. In addition, DNA damage, in the presence of CuCl<sub>2</sub> and H<sub>2</sub>O<sub>2</sub>, increased by adding both the father and son SNBP. Interestingly, son SNBP, unlike his father, showed an unstable DNA binding and were able to produce DNA damage even without external addition of CuCl<sub>2</sub>, in line with a lower seminal antioxidant activity than the father. The peculiarity of some characteristics of son semen could be a basis for possible future studies on transgenerational effects of pollutants on fertility.https://www.mdpi.com/1422-0067/21/18/6710human spermatozoahuman protaminesLand of FiresDNA oxidative damageprotein-DNA bindingheavy metals |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gennaro Lettieri Federica Marra Claudia Moriello Marina Prisco Tiziana Notari Marco Trifuoggi Antonella Giarra Liana Bosco Luigi Montano Marina Piscopo |
spellingShingle |
Gennaro Lettieri Federica Marra Claudia Moriello Marina Prisco Tiziana Notari Marco Trifuoggi Antonella Giarra Liana Bosco Luigi Montano Marina Piscopo Molecular Alterations in Spermatozoa of a Family Case Living in the Land of Fires. A First Look at Possible Transgenerational Effects of Pollutants International Journal of Molecular Sciences human spermatozoa human protamines Land of Fires DNA oxidative damage protein-DNA binding heavy metals |
author_facet |
Gennaro Lettieri Federica Marra Claudia Moriello Marina Prisco Tiziana Notari Marco Trifuoggi Antonella Giarra Liana Bosco Luigi Montano Marina Piscopo |
author_sort |
Gennaro Lettieri |
title |
Molecular Alterations in Spermatozoa of a Family Case Living in the Land of Fires. A First Look at Possible Transgenerational Effects of Pollutants |
title_short |
Molecular Alterations in Spermatozoa of a Family Case Living in the Land of Fires. A First Look at Possible Transgenerational Effects of Pollutants |
title_full |
Molecular Alterations in Spermatozoa of a Family Case Living in the Land of Fires. A First Look at Possible Transgenerational Effects of Pollutants |
title_fullStr |
Molecular Alterations in Spermatozoa of a Family Case Living in the Land of Fires. A First Look at Possible Transgenerational Effects of Pollutants |
title_full_unstemmed |
Molecular Alterations in Spermatozoa of a Family Case Living in the Land of Fires. A First Look at Possible Transgenerational Effects of Pollutants |
title_sort |
molecular alterations in spermatozoa of a family case living in the land of fires. a first look at possible transgenerational effects of pollutants |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-09-01 |
description |
In our previous work, we reported alterations in protamines/histones ratio, in DNA binding of these proteins and their involvement in DNA oxidative damage in 84% of the young men living in the Land of Fires. In the present work, we extended our findings, evaluating any alterations in spermatozoa of a family case, a father and son, living in this area, to also give a first look at the possibility of transgenerational inherited effects of environmental contaminants on the molecular alterations of sperm nuclear basic proteins (SNBP), DNA and semen parameters. In the father and son, we found a diverse excess of copper and chromium in the semen, different alterations in SNBP content and low DNA binding affinity of these proteins. In addition, DNA damage, in the presence of CuCl<sub>2</sub> and H<sub>2</sub>O<sub>2</sub>, increased by adding both the father and son SNBP. Interestingly, son SNBP, unlike his father, showed an unstable DNA binding and were able to produce DNA damage even without external addition of CuCl<sub>2</sub>, in line with a lower seminal antioxidant activity than the father. The peculiarity of some characteristics of son semen could be a basis for possible future studies on transgenerational effects of pollutants on fertility. |
topic |
human spermatozoa human protamines Land of Fires DNA oxidative damage protein-DNA binding heavy metals |
url |
https://www.mdpi.com/1422-0067/21/18/6710 |
work_keys_str_mv |
AT gennarolettieri molecularalterationsinspermatozoaofafamilycaselivinginthelandoffiresafirstlookatpossibletransgenerationaleffectsofpollutants AT federicamarra molecularalterationsinspermatozoaofafamilycaselivinginthelandoffiresafirstlookatpossibletransgenerationaleffectsofpollutants AT claudiamoriello molecularalterationsinspermatozoaofafamilycaselivinginthelandoffiresafirstlookatpossibletransgenerationaleffectsofpollutants AT marinaprisco molecularalterationsinspermatozoaofafamilycaselivinginthelandoffiresafirstlookatpossibletransgenerationaleffectsofpollutants AT tiziananotari molecularalterationsinspermatozoaofafamilycaselivinginthelandoffiresafirstlookatpossibletransgenerationaleffectsofpollutants AT marcotrifuoggi molecularalterationsinspermatozoaofafamilycaselivinginthelandoffiresafirstlookatpossibletransgenerationaleffectsofpollutants AT antonellagiarra molecularalterationsinspermatozoaofafamilycaselivinginthelandoffiresafirstlookatpossibletransgenerationaleffectsofpollutants AT lianabosco molecularalterationsinspermatozoaofafamilycaselivinginthelandoffiresafirstlookatpossibletransgenerationaleffectsofpollutants AT luigimontano molecularalterationsinspermatozoaofafamilycaselivinginthelandoffiresafirstlookatpossibletransgenerationaleffectsofpollutants AT marinapiscopo molecularalterationsinspermatozoaofafamilycaselivinginthelandoffiresafirstlookatpossibletransgenerationaleffectsofpollutants |
_version_ |
1725128536665096192 |