Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars

• Main Authors: Rosa Puchades, Ángel Maquieira, Carlos A. Barrios, David López-Romero, Miguel Holgado, María Fe Laguna, Rafael Casquel, Francisco J. Sanza, María-José Bañuls, Francisco J. Ortega
• Format: Article
• Language: English
• Published: MDPI AG 2012-08-01
• Series: Biosensors
• Subjects: micro-nanofabrication; SU-8; optical interrogation; label-free nanobiosensing; gestrinone; immunoassay affinity constant determination
• Online Access: http://www.mdpi.com/2079-6374/2/3/291

In this paper, label-free biosensing for antibody screening by periodic lattices of high-aspect ratio SU-8 nano-pillars (BICELLs) is presented. As a demonstration, the determination of anti-gestrinone antibodies from whole rabbit serum is carried out, and for the first time, the dissociation constant (KD = 6 nM) of antigen-antibody recognition process is calculated using this sensing system. After gestrinone antigen immobilization on the BICELLs, the immunorecognition was performed. The cells were interrogated vertically by using micron spot size Fourier transform visible and IR spectrometry (FT-VIS-IR), and the dip wavenumber shift was monitored. The biosensing assay exhibited good reproducibility and sensitivity (LOD = 0.75 ng/mL).