Continuous Separation of Circulating Tumor Cells from Whole Blood Using a Slanted Weir Microfluidic Device

Continuous Separation of Circulating Tumor Cells from Whole Blood Using a Slanted Weir Microfluidic Device

The separation of circulating tumor cells (CTCs) from the peripheral blood is an important issue that has been highlighted because of their high clinical potential. However, techniques that depend solely on tumor-specific surface molecules or just the larger size of CTCs are limited by tumor heterog...

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Main Authors: Yousang Yoon, Jusin Lee, Moonsoo Ra, Hyeokshin Gwon, Seungwon Lee, Min Young Kim, Ki-Chun Yoo, Onejae Sul, Chul Geun Kim, Whoi-Yul Kim, Jea-Gun Park, Su-Jae Lee, Young Yiul Lee, Ho Soon Choi, Seung-Beck Lee
Format: Article
Language:English
Published: MDPI AG 2019-02-01
Series:Cancers
Subjects:
circulating tumor cell
cell separation
microfluidics
Online Access:https://www.mdpi.com/2072-6694/11/2/200
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spelling doaj-fabad2c510ed4a2f8739c6e8dedeef552020-11-24T21:21:34ZengMDPI AGCancers2072-66942019-02-0111220010.3390/cancers11020200cancers11020200Continuous Separation of Circulating Tumor Cells from Whole Blood Using a Slanted Weir Microfluidic DeviceYousang Yoon0Jusin Lee1Moonsoo Ra2Hyeokshin Gwon3Seungwon Lee4Min Young Kim5Ki-Chun Yoo6Onejae Sul7Chul Geun Kim8Whoi-Yul Kim9Jea-Gun Park10Su-Jae Lee11Young Yiul Lee12Ho Soon Choi13Seung-Beck Lee14Department of Electronic Engineering, Hanyang University College of Engineering, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Electronic Engineering, Hanyang University College of Engineering, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Electronic Engineering, Hanyang University College of Engineering, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Electronic Engineering, Hanyang University College of Engineering, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Electronic Engineering, Hanyang University College of Engineering, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Life Science and Research Institute for Natural Sciences, Hanyang University College of Natural Sciences, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Life Science and Research Institute for Natural Sciences, Hanyang University College of Natural Sciences, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaInstitute of Nano Science and Technology, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Life Science and Research Institute for Natural Sciences, Hanyang University College of Natural Sciences, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Electronic Engineering, Hanyang University College of Engineering, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Electronic Engineering, Hanyang University College of Engineering, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Life Science and Research Institute for Natural Sciences, Hanyang University College of Natural Sciences, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Internal Medicine, Hanyang University College of Medicine, Hanyang University Medical Center, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Internal Medicine, Hanyang University College of Medicine, Hanyang University Medical Center, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Electronic Engineering, Hanyang University College of Engineering, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaThe separation of circulating tumor cells (CTCs) from the peripheral blood is an important issue that has been highlighted because of their high clinical potential. However, techniques that depend solely on tumor-specific surface molecules or just the larger size of CTCs are limited by tumor heterogeneity. Here, we present a slanted weir microfluidic device that utilizes the size and deformability of CTCs to separate them from the unprocessed whole blood. By testing its ability using a highly invasive breast cancer cell line, our device achieved a 97% separation efficiency, while showing an 8-log depletion of erythrocytes and 5.6-log depletion of leukocytes. We also developed an image analysis tool that was able to characterize the various morphologies and differing deformability of the separating cells. From the results, we believe our system possesses a high potential for liquid biopsy, aiding future cancer research.https://www.mdpi.com/2072-6694/11/2/200circulating tumor cellcell separationmicrofluidics
collection DOAJ
language English
format Article
sources DOAJ
author Yousang Yoon
Jusin Lee
Moonsoo Ra
Hyeokshin Gwon
Seungwon Lee
Min Young Kim
Ki-Chun Yoo
Onejae Sul
Chul Geun Kim
Whoi-Yul Kim
Jea-Gun Park
Su-Jae Lee
Young Yiul Lee
Ho Soon Choi
Seung-Beck Lee
spellingShingle Yousang Yoon
Jusin Lee
Moonsoo Ra
Hyeokshin Gwon
Seungwon Lee
Min Young Kim
Ki-Chun Yoo
Onejae Sul
Chul Geun Kim
Whoi-Yul Kim
Jea-Gun Park
Su-Jae Lee
Young Yiul Lee
Ho Soon Choi
Seung-Beck Lee
Continuous Separation of Circulating Tumor Cells from Whole Blood Using a Slanted Weir Microfluidic Device
Cancers
circulating tumor cell
cell separation
microfluidics
author_facet Yousang Yoon
Jusin Lee
Moonsoo Ra
Hyeokshin Gwon
Seungwon Lee
Min Young Kim
Ki-Chun Yoo
Onejae Sul
Chul Geun Kim
Whoi-Yul Kim
Jea-Gun Park
Su-Jae Lee
Young Yiul Lee
Ho Soon Choi
Seung-Beck Lee
author_sort Yousang Yoon
title Continuous Separation of Circulating Tumor Cells from Whole Blood Using a Slanted Weir Microfluidic Device
title_short Continuous Separation of Circulating Tumor Cells from Whole Blood Using a Slanted Weir Microfluidic Device
title_full Continuous Separation of Circulating Tumor Cells from Whole Blood Using a Slanted Weir Microfluidic Device
title_fullStr Continuous Separation of Circulating Tumor Cells from Whole Blood Using a Slanted Weir Microfluidic Device
title_full_unstemmed Continuous Separation of Circulating Tumor Cells from Whole Blood Using a Slanted Weir Microfluidic Device
title_sort continuous separation of circulating tumor cells from whole blood using a slanted weir microfluidic device
publisher MDPI AG
series Cancers
issn 2072-6694
publishDate 2019-02-01
description The separation of circulating tumor cells (CTCs) from the peripheral blood is an important issue that has been highlighted because of their high clinical potential. However, techniques that depend solely on tumor-specific surface molecules or just the larger size of CTCs are limited by tumor heterogeneity. Here, we present a slanted weir microfluidic device that utilizes the size and deformability of CTCs to separate them from the unprocessed whole blood. By testing its ability using a highly invasive breast cancer cell line, our device achieved a 97% separation efficiency, while showing an 8-log depletion of erythrocytes and 5.6-log depletion of leukocytes. We also developed an image analysis tool that was able to characterize the various morphologies and differing deformability of the separating cells. From the results, we believe our system possesses a high potential for liquid biopsy, aiding future cancer research.
topic circulating tumor cell
cell separation
microfluidics
url https://www.mdpi.com/2072-6694/11/2/200
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