Biofilm reduction potential of 0.02% polyhexanide irrigation solution in several types of urethral catheters
Abstract Background Long-term use of urethral catheters is associated with high risk of urinary tract infection (UTI) and blockage. Microbial biofilms are a common cause of catheter blockage, reducing their lifetime and significantly increasing morbidity of UTIs. A 0.02% polyhexanide irrigation solu...
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doaj-fabd82026df7479693dbbce4151ec56f2021-04-11T11:24:55ZengBMCBMC Urology1471-24902021-04-012111610.1186/s12894-021-00826-3Biofilm reduction potential of 0.02% polyhexanide irrigation solution in several types of urethral cathetersFlorian H. H. Brill0Julia Hambach1Christian Utpatel2Diana C. Mogrovejo3Henrik Gabriel4Jan-Hendrik Klock5Joerg Steinmann6Andreas Arndt7Dr. Brill + Partner GmbH Institute for Hygiene and MicrobiologyDr. Brill + Partner GmbH Institute for Hygiene and MicrobiologyMolecular and Experimental Mycobacteriology, Research Center Borstel – Leibniz Lung CenterDr. Brill + Partner GmbH Institute for Hygiene and MicrobiologyDr. Brill + Partner GmbH Institute for Hygiene and MicrobiologyDr. Brill + Partner GmbH Institute for Hygiene and MicrobiologyKlinikum Nürnberg, Institute of Clinical Hygiene, Medical Microbiology and Infectiology, Paracelsus Medical UniversityDepartment of Research and Development, B. Braun Medical Ltd.Abstract Background Long-term use of urethral catheters is associated with high risk of urinary tract infection (UTI) and blockage. Microbial biofilms are a common cause of catheter blockage, reducing their lifetime and significantly increasing morbidity of UTIs. A 0.02% polyhexanide irrigation solution developed for routine mechanical rinsing shows potential for bacterial decolonization of urethral catheters and has the potential to reduce or prevent biofilm formation. Methods Using an in vitro assay with standard market-leading types of catheters artificially contaminated with clinically relevant bacteria, assays were carried out to evaluate the biofilm reduction and prevention potential of a 0.02% polyhexanide solution versus no intervention (standard approach) and irrigation with saline solution (NaCl 0.9%). The efficiency of decolonization was measured through microbial plate count and membrane filtration. Results Irrigation using a 0.02% polyhexanide solution is suitable for the decolonization of a variety of transurethral catheters. The effect observed is significant compared to irrigation with 0.9% saline solution (p = 0.002) or no treatment (p = 0.011). No significant difference was found between irrigation with 0.9% saline solution and no treatment (p = 0.74). Conclusions A 0.02% polyhexanide solution is able to reduce bacterial biofilm from catheters artificially contaminated with clinically relevant bacteria in vitro. The data shows a reduction of the viability of thick bacterial biofilms in a variety of commercially available urinary catheters made from silicone, latex-free silicone, hydrogel-coated silicone and PVC. Further research is required to evaluate the long-term tolerability and efficacy of polyhexanide in clinical practice.https://doi.org/10.1186/s12894-021-00826-3Bacterial decolonizationBiofilmPolyhexanideUrinary catheterUrinary tract infectionTransurethral catheter |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Florian H. H. Brill Julia Hambach Christian Utpatel Diana C. Mogrovejo Henrik Gabriel Jan-Hendrik Klock Joerg Steinmann Andreas Arndt |
spellingShingle |
Florian H. H. Brill Julia Hambach Christian Utpatel Diana C. Mogrovejo Henrik Gabriel Jan-Hendrik Klock Joerg Steinmann Andreas Arndt Biofilm reduction potential of 0.02% polyhexanide irrigation solution in several types of urethral catheters BMC Urology Bacterial decolonization Biofilm Polyhexanide Urinary catheter Urinary tract infection Transurethral catheter |
author_facet |
Florian H. H. Brill Julia Hambach Christian Utpatel Diana C. Mogrovejo Henrik Gabriel Jan-Hendrik Klock Joerg Steinmann Andreas Arndt |
author_sort |
Florian H. H. Brill |
title |
Biofilm reduction potential of 0.02% polyhexanide irrigation solution in several types of urethral catheters |
title_short |
Biofilm reduction potential of 0.02% polyhexanide irrigation solution in several types of urethral catheters |
title_full |
Biofilm reduction potential of 0.02% polyhexanide irrigation solution in several types of urethral catheters |
title_fullStr |
Biofilm reduction potential of 0.02% polyhexanide irrigation solution in several types of urethral catheters |
title_full_unstemmed |
Biofilm reduction potential of 0.02% polyhexanide irrigation solution in several types of urethral catheters |
title_sort |
biofilm reduction potential of 0.02% polyhexanide irrigation solution in several types of urethral catheters |
publisher |
BMC |
series |
BMC Urology |
issn |
1471-2490 |
publishDate |
2021-04-01 |
description |
Abstract Background Long-term use of urethral catheters is associated with high risk of urinary tract infection (UTI) and blockage. Microbial biofilms are a common cause of catheter blockage, reducing their lifetime and significantly increasing morbidity of UTIs. A 0.02% polyhexanide irrigation solution developed for routine mechanical rinsing shows potential for bacterial decolonization of urethral catheters and has the potential to reduce or prevent biofilm formation. Methods Using an in vitro assay with standard market-leading types of catheters artificially contaminated with clinically relevant bacteria, assays were carried out to evaluate the biofilm reduction and prevention potential of a 0.02% polyhexanide solution versus no intervention (standard approach) and irrigation with saline solution (NaCl 0.9%). The efficiency of decolonization was measured through microbial plate count and membrane filtration. Results Irrigation using a 0.02% polyhexanide solution is suitable for the decolonization of a variety of transurethral catheters. The effect observed is significant compared to irrigation with 0.9% saline solution (p = 0.002) or no treatment (p = 0.011). No significant difference was found between irrigation with 0.9% saline solution and no treatment (p = 0.74). Conclusions A 0.02% polyhexanide solution is able to reduce bacterial biofilm from catheters artificially contaminated with clinically relevant bacteria in vitro. The data shows a reduction of the viability of thick bacterial biofilms in a variety of commercially available urinary catheters made from silicone, latex-free silicone, hydrogel-coated silicone and PVC. Further research is required to evaluate the long-term tolerability and efficacy of polyhexanide in clinical practice. |
topic |
Bacterial decolonization Biofilm Polyhexanide Urinary catheter Urinary tract infection Transurethral catheter |
url |
https://doi.org/10.1186/s12894-021-00826-3 |
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