Identification of Streptococcus Mutans Isolated from Dental Plaques Based on the Presence of gtfB Gene

• Main Authors: Shahzad Rahmandoost, Kumarss Amini
• Format: Article
• Language: fas
• Published: Vesnu Publications 2015-12-01
• Series: مجله دانشکده پزشکی اصفهان
• Subjects: Streptococcus mutans; Glucosyltransferase; gtfB gene
• Online Access: http://jims.mui.ac.ir/index.php/jims/article/view/4826

Background: Oral streptococci, especially Streptococcus mutans, are of the factors identified to cause tooth decay and periodontal disease. Glucosyltransferase gene (gtfB) encoded by the GTF-I is an important factor in the virulence of the bacteria. Now, the development of molecular methods tends to detect Streptococcus mutans with accuracy and speed. This study aimed to identify Streptococcus mutans in human dental plaques based on the presence of gtfB gene. Methods: After collecting 55 swab samples of dental plaques, biochemical and microbiological tests were performed. DNA samples were confirmed using the extraction kit and then, the polymerase chain reaction (PCR) assay was performed on samples to identify the gtfB gene. Findings: Of 55 isolates collected from dental plaques, 17 samples (30.9%) were positive for the presence of gtfB gene. Conclusion: The results obtained in this study found to have differences with other researches conducted in other countries and regions of Iran. These differences may be due to cultural differences in the use of toothbrush and dental floss, diet and oral health status.