Regeneration of Solanum nigrum by somatic embryogenesis, involving frog egg-like body, a novel structure.
A new protocol was established for the regeneration of Solanum nigrum by frog egg-like bodies (FELBs), which are novel somatic embryogenesis (SE) structures induced from the root, stem, and leaf explants. The root, stem, and leaf explants (93.33%, 85.10%, and 100.00%, respectively) were induced to f...
Main Authors: | , , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4045584?pdf=render |
id |
doaj-fad0f7c56092439e95f854f7c6e05fcd |
---|---|
record_format |
Article |
spelling |
doaj-fad0f7c56092439e95f854f7c6e05fcd2020-11-25T01:52:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e9867210.1371/journal.pone.0098672Regeneration of Solanum nigrum by somatic embryogenesis, involving frog egg-like body, a novel structure.Kedong XuYunxia ChangKun LiuFeige WangZhongyuan LiuTing ZhangTong LiYi ZhangFuli ZhangJu ZhangYan WangWei NiuShuzhao JiaHengchang XieGuangxuan TanChengwei LiA new protocol was established for the regeneration of Solanum nigrum by frog egg-like bodies (FELBs), which are novel somatic embryogenesis (SE) structures induced from the root, stem, and leaf explants. The root, stem, and leaf explants (93.33%, 85.10%, and 100.00%, respectively) were induced to form special embryonic calli on Murashige and Skoog (MS) medium containing 1.0 mg/L 2,4-dichlorophenoxyacetic acid, under dark condition. Further, special embryonic calli from the root, stem, and leaf explants (86.97%, 83.30%, and 99.47%, respectively) were developed into FELBs. Plantlets of FELBs from the three explants were induced in vitro on MS medium supplemented with 5.0 mg/L 6-benzylaminopurine and 0.1 mg/L gibberellic acid, and 100.00% plantlet induction rates were noted. However, plantlet induction in vivo on MS medium supplemented with 20 mg/L thidiazuron showed rates of 38.63%, 15.63%, and 61.30% for the root, stem, and leaf explants, respectively, which were lower than those of the in vitro culture. Morphological and histological analyses of FELBs at different development stages revealed that they are a novel type of SE structure that developed from the mesophyll (leaf) or cortex (stem and root) cells of S. nigrum.http://europepmc.org/articles/PMC4045584?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kedong Xu Yunxia Chang Kun Liu Feige Wang Zhongyuan Liu Ting Zhang Tong Li Yi Zhang Fuli Zhang Ju Zhang Yan Wang Wei Niu Shuzhao Jia Hengchang Xie Guangxuan Tan Chengwei Li |
spellingShingle |
Kedong Xu Yunxia Chang Kun Liu Feige Wang Zhongyuan Liu Ting Zhang Tong Li Yi Zhang Fuli Zhang Ju Zhang Yan Wang Wei Niu Shuzhao Jia Hengchang Xie Guangxuan Tan Chengwei Li Regeneration of Solanum nigrum by somatic embryogenesis, involving frog egg-like body, a novel structure. PLoS ONE |
author_facet |
Kedong Xu Yunxia Chang Kun Liu Feige Wang Zhongyuan Liu Ting Zhang Tong Li Yi Zhang Fuli Zhang Ju Zhang Yan Wang Wei Niu Shuzhao Jia Hengchang Xie Guangxuan Tan Chengwei Li |
author_sort |
Kedong Xu |
title |
Regeneration of Solanum nigrum by somatic embryogenesis, involving frog egg-like body, a novel structure. |
title_short |
Regeneration of Solanum nigrum by somatic embryogenesis, involving frog egg-like body, a novel structure. |
title_full |
Regeneration of Solanum nigrum by somatic embryogenesis, involving frog egg-like body, a novel structure. |
title_fullStr |
Regeneration of Solanum nigrum by somatic embryogenesis, involving frog egg-like body, a novel structure. |
title_full_unstemmed |
Regeneration of Solanum nigrum by somatic embryogenesis, involving frog egg-like body, a novel structure. |
title_sort |
regeneration of solanum nigrum by somatic embryogenesis, involving frog egg-like body, a novel structure. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
A new protocol was established for the regeneration of Solanum nigrum by frog egg-like bodies (FELBs), which are novel somatic embryogenesis (SE) structures induced from the root, stem, and leaf explants. The root, stem, and leaf explants (93.33%, 85.10%, and 100.00%, respectively) were induced to form special embryonic calli on Murashige and Skoog (MS) medium containing 1.0 mg/L 2,4-dichlorophenoxyacetic acid, under dark condition. Further, special embryonic calli from the root, stem, and leaf explants (86.97%, 83.30%, and 99.47%, respectively) were developed into FELBs. Plantlets of FELBs from the three explants were induced in vitro on MS medium supplemented with 5.0 mg/L 6-benzylaminopurine and 0.1 mg/L gibberellic acid, and 100.00% plantlet induction rates were noted. However, plantlet induction in vivo on MS medium supplemented with 20 mg/L thidiazuron showed rates of 38.63%, 15.63%, and 61.30% for the root, stem, and leaf explants, respectively, which were lower than those of the in vitro culture. Morphological and histological analyses of FELBs at different development stages revealed that they are a novel type of SE structure that developed from the mesophyll (leaf) or cortex (stem and root) cells of S. nigrum. |
url |
http://europepmc.org/articles/PMC4045584?pdf=render |
work_keys_str_mv |
AT kedongxu regenerationofsolanumnigrumbysomaticembryogenesisinvolvingfrogegglikebodyanovelstructure AT yunxiachang regenerationofsolanumnigrumbysomaticembryogenesisinvolvingfrogegglikebodyanovelstructure AT kunliu regenerationofsolanumnigrumbysomaticembryogenesisinvolvingfrogegglikebodyanovelstructure AT feigewang regenerationofsolanumnigrumbysomaticembryogenesisinvolvingfrogegglikebodyanovelstructure AT zhongyuanliu regenerationofsolanumnigrumbysomaticembryogenesisinvolvingfrogegglikebodyanovelstructure AT tingzhang regenerationofsolanumnigrumbysomaticembryogenesisinvolvingfrogegglikebodyanovelstructure AT tongli regenerationofsolanumnigrumbysomaticembryogenesisinvolvingfrogegglikebodyanovelstructure AT yizhang regenerationofsolanumnigrumbysomaticembryogenesisinvolvingfrogegglikebodyanovelstructure AT fulizhang regenerationofsolanumnigrumbysomaticembryogenesisinvolvingfrogegglikebodyanovelstructure AT juzhang regenerationofsolanumnigrumbysomaticembryogenesisinvolvingfrogegglikebodyanovelstructure AT yanwang regenerationofsolanumnigrumbysomaticembryogenesisinvolvingfrogegglikebodyanovelstructure AT weiniu regenerationofsolanumnigrumbysomaticembryogenesisinvolvingfrogegglikebodyanovelstructure AT shuzhaojia regenerationofsolanumnigrumbysomaticembryogenesisinvolvingfrogegglikebodyanovelstructure AT hengchangxie regenerationofsolanumnigrumbysomaticembryogenesisinvolvingfrogegglikebodyanovelstructure AT guangxuantan regenerationofsolanumnigrumbysomaticembryogenesisinvolvingfrogegglikebodyanovelstructure AT chengweili regenerationofsolanumnigrumbysomaticembryogenesisinvolvingfrogegglikebodyanovelstructure |
_version_ |
1724993290911088640 |