Flow immunosensor system with an electrode replacement unit for continuous cortisol monitoring for fish
Blood cortisol concentration change in fish is widely used as an indicator of stress. In this study, we developed a flow system which equipped by an electrode replacement unit which can replace sensor conveniently. Multiple sensors sequentially disposed vertically in the holder, and the conductive p...
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doaj-fad5e0c0c3634fffa8b19bb1c31d26762020-11-24T23:14:27ZengElsevierSensing and Bio-Sensing Research2214-18042017-04-0113122127Flow immunosensor system with an electrode replacement unit for continuous cortisol monitoring for fishHaiyun Wu0Hitoshi Ohnuki1Masataka Murata2Hideaki Endo3Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo 108-8477, JapanGraduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 2-1-6, Etchujima, Koto-ku, Tokyo 135-8533, JapanHokkaido Industrial Technology Center, 379 Kikyo-cho, Hakodate, Hokkaido 041-0801, JapanGraduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo 108-8477, Japan; Corresponding author.Blood cortisol concentration change in fish is widely used as an indicator of stress. In this study, we developed a flow system which equipped by an electrode replacement unit which can replace sensor conveniently. Multiple sensors sequentially disposed vertically in the holder, and the conductive portion of the sensor to be used was bonded with which set in the bottom of the holder. After measurement, next sensor was inserted into the holder and the replacement would be completed by sequentially moving. The flow system was constructed by replaceable working electrodes which sequence placed in the electrode replacement unit and count electrode into a reaction loop which operated by micro-pump. During the measurement, the potassium ferricyanide was transported into the flow loop and applied a voltage (+250 mV). Output current values were measured both before and after the immune-reaction and the change between them was calculated to determine the cortisol levels. As a result, replacement of the immunosensor performed in only several seconds and a good linear relationship was observed between the sensor response and cortisol in the range of 0–40 ng ml−1. We also performed a measurement of cortisol levels in plasma of tilapia (Oreochromis niloticus) both by proposed biosensor system and ELISA which got a good correlation. We believe that the proposed system can provide a simple and rapid detection of cortisol levels for fish and its principle could be a foundation of a continuous in vivo monitoring of fish cortisol change in fish in the future. Keywords: Electrode replacement unit, Immunosensor, Flow system, Cortisol, Fish stresshttp://www.sciencedirect.com/science/article/pii/S2214180417300120 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Haiyun Wu Hitoshi Ohnuki Masataka Murata Hideaki Endo |
spellingShingle |
Haiyun Wu Hitoshi Ohnuki Masataka Murata Hideaki Endo Flow immunosensor system with an electrode replacement unit for continuous cortisol monitoring for fish Sensing and Bio-Sensing Research |
author_facet |
Haiyun Wu Hitoshi Ohnuki Masataka Murata Hideaki Endo |
author_sort |
Haiyun Wu |
title |
Flow immunosensor system with an electrode replacement unit for continuous cortisol monitoring for fish |
title_short |
Flow immunosensor system with an electrode replacement unit for continuous cortisol monitoring for fish |
title_full |
Flow immunosensor system with an electrode replacement unit for continuous cortisol monitoring for fish |
title_fullStr |
Flow immunosensor system with an electrode replacement unit for continuous cortisol monitoring for fish |
title_full_unstemmed |
Flow immunosensor system with an electrode replacement unit for continuous cortisol monitoring for fish |
title_sort |
flow immunosensor system with an electrode replacement unit for continuous cortisol monitoring for fish |
publisher |
Elsevier |
series |
Sensing and Bio-Sensing Research |
issn |
2214-1804 |
publishDate |
2017-04-01 |
description |
Blood cortisol concentration change in fish is widely used as an indicator of stress. In this study, we developed a flow system which equipped by an electrode replacement unit which can replace sensor conveniently. Multiple sensors sequentially disposed vertically in the holder, and the conductive portion of the sensor to be used was bonded with which set in the bottom of the holder. After measurement, next sensor was inserted into the holder and the replacement would be completed by sequentially moving. The flow system was constructed by replaceable working electrodes which sequence placed in the electrode replacement unit and count electrode into a reaction loop which operated by micro-pump. During the measurement, the potassium ferricyanide was transported into the flow loop and applied a voltage (+250 mV). Output current values were measured both before and after the immune-reaction and the change between them was calculated to determine the cortisol levels. As a result, replacement of the immunosensor performed in only several seconds and a good linear relationship was observed between the sensor response and cortisol in the range of 0–40 ng ml−1. We also performed a measurement of cortisol levels in plasma of tilapia (Oreochromis niloticus) both by proposed biosensor system and ELISA which got a good correlation. We believe that the proposed system can provide a simple and rapid detection of cortisol levels for fish and its principle could be a foundation of a continuous in vivo monitoring of fish cortisol change in fish in the future. Keywords: Electrode replacement unit, Immunosensor, Flow system, Cortisol, Fish stress |
url |
http://www.sciencedirect.com/science/article/pii/S2214180417300120 |
work_keys_str_mv |
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