Hepatoprotective Effect of Pretreatment with Thymus vulgaris Essential Oil in Experimental Model of Acetaminophen-Induced Injury
Acute liver damage caused by acetaminophen overdose is a significant clinical problem and could benefit from new therapeutic strategies. Objective. This study investigated the hepatoprotective effect of Thymus vulgaris essential oil (TEO), which is used popularly for various beneficial effects, such...
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doaj-fae355be01f2426ab13150e1ce4911a72020-11-24T23:14:27ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882014-01-01201410.1155/2014/954136954136Hepatoprotective Effect of Pretreatment with Thymus vulgaris Essential Oil in Experimental Model of Acetaminophen-Induced InjuryRenata Grespan0Rafael Pazinatto Aguiar1Frederico Nunes Giubilei2Rafael Rocco Fuso3Marcio José Damião4Expedito Leite Silva5Jane Graton Mikcha6Luzmarina Hernandes7Ciomar Bersani Amado8Roberto Kenji Nakamura Cuman9Department of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, BrazilDepartment of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, BrazilDepartment of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, BrazilDepartment of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, BrazilDepartment of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, BrazilDepartment of Chemistry, State University of Maringá, 87020-900 Maringá, PR, BrazilDepartment of Clinical Analysis, State University of Maringá, 87020-900 Maringá, PR, BrazilDepartment of Morphophysiology Sciences, State University of Maringá, 87020-900 Maringá, PR, BrazilDepartment of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, BrazilDepartment of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, BrazilAcute liver damage caused by acetaminophen overdose is a significant clinical problem and could benefit from new therapeutic strategies. Objective. This study investigated the hepatoprotective effect of Thymus vulgaris essential oil (TEO), which is used popularly for various beneficial effects, such as its antiseptic, carminative, and antimicrobial effects. The hepatoprotective activity of TEO was determined by assessing serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) in mice. Their livers were then used to determine myeloperoxidase (MPO) enzyme activity and subjected to histological analysis. In vitro antioxidant activity was evaluated by assessing the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•)-scavenging effects of TEO and TEO-induced lipid peroxidation. TEO reduced the levels of the serum marker enzymes AST, ALT, and ALP and MPO activity. The histopathological analysis indicated that TEO prevented acetaminophen-induced necrosis. The essential oil also exhibited antioxidant activity, reflected by its DPPH radical-scavenging effects and in the lipid peroxidation assay. These results suggest that TEO has hepatoprotective effects on acetaminophen-induced hepatic damage in mice.http://dx.doi.org/10.1155/2014/954136 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Renata Grespan Rafael Pazinatto Aguiar Frederico Nunes Giubilei Rafael Rocco Fuso Marcio José Damião Expedito Leite Silva Jane Graton Mikcha Luzmarina Hernandes Ciomar Bersani Amado Roberto Kenji Nakamura Cuman |
spellingShingle |
Renata Grespan Rafael Pazinatto Aguiar Frederico Nunes Giubilei Rafael Rocco Fuso Marcio José Damião Expedito Leite Silva Jane Graton Mikcha Luzmarina Hernandes Ciomar Bersani Amado Roberto Kenji Nakamura Cuman Hepatoprotective Effect of Pretreatment with Thymus vulgaris Essential Oil in Experimental Model of Acetaminophen-Induced Injury Evidence-Based Complementary and Alternative Medicine |
author_facet |
Renata Grespan Rafael Pazinatto Aguiar Frederico Nunes Giubilei Rafael Rocco Fuso Marcio José Damião Expedito Leite Silva Jane Graton Mikcha Luzmarina Hernandes Ciomar Bersani Amado Roberto Kenji Nakamura Cuman |
author_sort |
Renata Grespan |
title |
Hepatoprotective Effect of Pretreatment with Thymus vulgaris Essential Oil in Experimental Model of Acetaminophen-Induced Injury |
title_short |
Hepatoprotective Effect of Pretreatment with Thymus vulgaris Essential Oil in Experimental Model of Acetaminophen-Induced Injury |
title_full |
Hepatoprotective Effect of Pretreatment with Thymus vulgaris Essential Oil in Experimental Model of Acetaminophen-Induced Injury |
title_fullStr |
Hepatoprotective Effect of Pretreatment with Thymus vulgaris Essential Oil in Experimental Model of Acetaminophen-Induced Injury |
title_full_unstemmed |
Hepatoprotective Effect of Pretreatment with Thymus vulgaris Essential Oil in Experimental Model of Acetaminophen-Induced Injury |
title_sort |
hepatoprotective effect of pretreatment with thymus vulgaris essential oil in experimental model of acetaminophen-induced injury |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2014-01-01 |
description |
Acute liver damage caused by acetaminophen overdose is a significant clinical problem and could benefit from new therapeutic strategies. Objective. This study investigated the hepatoprotective effect of Thymus vulgaris essential oil (TEO), which is used popularly for various beneficial effects, such as its antiseptic, carminative, and antimicrobial effects. The hepatoprotective activity of TEO was determined by assessing serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) in mice. Their livers were then used to determine myeloperoxidase (MPO) enzyme activity and subjected to histological analysis. In vitro antioxidant activity was evaluated by assessing the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•)-scavenging effects of TEO and TEO-induced lipid peroxidation. TEO reduced the levels of the serum marker enzymes AST, ALT, and ALP and MPO activity. The histopathological analysis indicated that TEO prevented acetaminophen-induced necrosis. The essential oil also exhibited antioxidant activity, reflected by its DPPH radical-scavenging effects and in the lipid peroxidation assay. These results suggest that TEO has hepatoprotective effects on acetaminophen-induced hepatic damage in mice. |
url |
http://dx.doi.org/10.1155/2014/954136 |
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