Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of...
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Instituto Oswaldo Cruz, Ministério da Saúde
2009-07-01
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doaj-fb19a66dcdb948fb9002c34f45bacb762020-11-25T00:32:46ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.0074-02761678-80602009-07-01104455856610.1590/S0074-02762009000400004Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in ThailandSorawat ThongsahuanVisut BaimaiYasushi OtsukaAtiporn SaeungBenjawan TuetunNarissara JariyapanSittiporn SuwannamitPradya SomboonAtchariya JitpakdiHiroyuki TakaokaWej ChoochoteSeventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of An. campestris (17-58 branches). They showed three different karyotypes based on the amount of extra heterochromatin in the sex chromosomes, namely Forms B (X2, Y2), E (X1, X2, X3, Y5) and a new karyotypic Form F (X2, X3, Y6). Form B has been found only in Chaing Mai and Kamphaeng Phet populations, while Forms E and F are widely distributed throughout the species range. Genetic crosses between the 12 isolines, which were arbitrarily selected as representatives of An. campestris-like Forms B, E and F, revealed genetic compatibility that provided viable progeny through F2 generations, suggesting a conspecific nature of these karyotypic forms. These results are supported by the very low intraspecies variation (genetic distance < 0.005) of ITS2, COI and COII from genomic DNA of the three karyotypic forms.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000400004Anopheles campestris-likemetaphase karyotypecrossing experimentITS2COICOII |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sorawat Thongsahuan Visut Baimai Yasushi Otsuka Atiporn Saeung Benjawan Tuetun Narissara Jariyapan Sittiporn Suwannamit Pradya Somboon Atchariya Jitpakdi Hiroyuki Takaoka Wej Choochote |
spellingShingle |
Sorawat Thongsahuan Visut Baimai Yasushi Otsuka Atiporn Saeung Benjawan Tuetun Narissara Jariyapan Sittiporn Suwannamit Pradya Somboon Atchariya Jitpakdi Hiroyuki Takaoka Wej Choochote Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand Memórias do Instituto Oswaldo Cruz. Anopheles campestris-like metaphase karyotype crossing experiment ITS2 COI COII |
author_facet |
Sorawat Thongsahuan Visut Baimai Yasushi Otsuka Atiporn Saeung Benjawan Tuetun Narissara Jariyapan Sittiporn Suwannamit Pradya Somboon Atchariya Jitpakdi Hiroyuki Takaoka Wej Choochote |
author_sort |
Sorawat Thongsahuan |
title |
Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand |
title_short |
Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand |
title_full |
Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand |
title_fullStr |
Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand |
title_full_unstemmed |
Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand |
title_sort |
karyotypic variation and geographic distribution of anopheles campestris-like (diptera: culicidae) in thailand |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
0074-0276 1678-8060 |
publishDate |
2009-07-01 |
description |
Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of An. campestris (17-58 branches). They showed three different karyotypes based on the amount of extra heterochromatin in the sex chromosomes, namely Forms B (X2, Y2), E (X1, X2, X3, Y5) and a new karyotypic Form F (X2, X3, Y6). Form B has been found only in Chaing Mai and Kamphaeng Phet populations, while Forms E and F are widely distributed throughout the species range. Genetic crosses between the 12 isolines, which were arbitrarily selected as representatives of An. campestris-like Forms B, E and F, revealed genetic compatibility that provided viable progeny through F2 generations, suggesting a conspecific nature of these karyotypic forms. These results are supported by the very low intraspecies variation (genetic distance < 0.005) of ITS2, COI and COII from genomic DNA of the three karyotypic forms. |
topic |
Anopheles campestris-like metaphase karyotype crossing experiment ITS2 COI COII |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000400004 |
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