Discovery of Candidate Stool Biomarker Proteins for Biliary Atresia Using Proteome Analysis by Data-Independent Acquisition Mass Spectrometry
Biliary atresia (BA) is a destructive inflammatory obliterative cholangiopathy of the neonate that affects various parts of the bile duct. If early diagnosis followed by Kasai portoenterostomy is not performed, progressive liver cirrhosis frequently leads to liver transplantation in the early stage...
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doaj-fb2cd846e16b410baa8ed5fad0c95f2d2020-11-28T00:01:55ZengMDPI AGProteomes2227-73822020-11-018363610.3390/proteomes8040036Discovery of Candidate Stool Biomarker Proteins for Biliary Atresia Using Proteome Analysis by Data-Independent Acquisition Mass SpectrometryEiichiro Watanabe0Yusuke Kawashima1Wataru Suda2Tomo Kakihara3Shinya Takazawa4Daisuke Nakajima5Ren Nakamura6Akira Nishi7Kan Suzuki8Osamu Ohara9Jun Fujishiro10Department of Pediatric Surgery, Faculty of Medicine, The University of Tokyo, Tokyo 113-8655, JapanDepartment of Applied Genomics, Kazusa DNA Research Institute, Kisarazu 292-0818, JapanLaboratory for Microbiome Sciences, RIKEN Center for Integrative Medical Sciences, Yokohama 230-0045, JapanDepartment of Pediatric Surgery, Faculty of Medicine, The University of Tokyo, Tokyo 113-8655, JapanDepartment of Surgery, Gunma Children’s Medical Center, Shibukawa 277-8577, JapanDepartment of Applied Genomics, Kazusa DNA Research Institute, Kisarazu 292-0818, JapanDepartment of Applied Genomics, Kazusa DNA Research Institute, Kisarazu 292-0818, JapanDepartment of Surgery, Gunma Children’s Medical Center, Shibukawa 277-8577, JapanDepartment of Pediatric Surgery, Faculty of Medicine, The University of Tokyo, Tokyo 113-8655, JapanDepartment of Applied Genomics, Kazusa DNA Research Institute, Kisarazu 292-0818, JapanDepartment of Pediatric Surgery, Faculty of Medicine, The University of Tokyo, Tokyo 113-8655, JapanBiliary atresia (BA) is a destructive inflammatory obliterative cholangiopathy of the neonate that affects various parts of the bile duct. If early diagnosis followed by Kasai portoenterostomy is not performed, progressive liver cirrhosis frequently leads to liver transplantation in the early stage of life. Therefore, prompt diagnosis is necessary for the rescue of BA patients. However, the prompt diagnosis of BA remains challenging because specific and reliable biomarkers for BA are currently unavailable. In this study, we discovered potential biomarkers for BA using deep proteome analysis by data-independent acquisition mass spectrometry (DIA–MS). Four patients with BA and three patients with neonatal cholestasis of other etiologies (non-BA) were recruited for stool proteome analysis. Among the 2110 host-derived proteins detected in their stools, 49 proteins were significantly higher in patients with BA and 54 proteins were significantly lower. These varying stool protein levels in infants with BA can provide potential biomarkers for BA. As demonstrated in this study, the deep proteome analysis of stools has great potential not only in detecting new stool biomarkers for BA but also in elucidating the pathophysiology of BA and other pediatric diseases, especially in the field of pediatric gastroenterology.https://www.mdpi.com/2227-7382/8/4/36biliary atresiaproteome analysisDIA–MSstool biomarker |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eiichiro Watanabe Yusuke Kawashima Wataru Suda Tomo Kakihara Shinya Takazawa Daisuke Nakajima Ren Nakamura Akira Nishi Kan Suzuki Osamu Ohara Jun Fujishiro |
spellingShingle |
Eiichiro Watanabe Yusuke Kawashima Wataru Suda Tomo Kakihara Shinya Takazawa Daisuke Nakajima Ren Nakamura Akira Nishi Kan Suzuki Osamu Ohara Jun Fujishiro Discovery of Candidate Stool Biomarker Proteins for Biliary Atresia Using Proteome Analysis by Data-Independent Acquisition Mass Spectrometry Proteomes biliary atresia proteome analysis DIA–MS stool biomarker |
author_facet |
Eiichiro Watanabe Yusuke Kawashima Wataru Suda Tomo Kakihara Shinya Takazawa Daisuke Nakajima Ren Nakamura Akira Nishi Kan Suzuki Osamu Ohara Jun Fujishiro |
author_sort |
Eiichiro Watanabe |
title |
Discovery of Candidate Stool Biomarker Proteins for Biliary Atresia Using Proteome Analysis by Data-Independent Acquisition Mass Spectrometry |
title_short |
Discovery of Candidate Stool Biomarker Proteins for Biliary Atresia Using Proteome Analysis by Data-Independent Acquisition Mass Spectrometry |
title_full |
Discovery of Candidate Stool Biomarker Proteins for Biliary Atresia Using Proteome Analysis by Data-Independent Acquisition Mass Spectrometry |
title_fullStr |
Discovery of Candidate Stool Biomarker Proteins for Biliary Atresia Using Proteome Analysis by Data-Independent Acquisition Mass Spectrometry |
title_full_unstemmed |
Discovery of Candidate Stool Biomarker Proteins for Biliary Atresia Using Proteome Analysis by Data-Independent Acquisition Mass Spectrometry |
title_sort |
discovery of candidate stool biomarker proteins for biliary atresia using proteome analysis by data-independent acquisition mass spectrometry |
publisher |
MDPI AG |
series |
Proteomes |
issn |
2227-7382 |
publishDate |
2020-11-01 |
description |
Biliary atresia (BA) is a destructive inflammatory obliterative cholangiopathy of the neonate that affects various parts of the bile duct. If early diagnosis followed by Kasai portoenterostomy is not performed, progressive liver cirrhosis frequently leads to liver transplantation in the early stage of life. Therefore, prompt diagnosis is necessary for the rescue of BA patients. However, the prompt diagnosis of BA remains challenging because specific and reliable biomarkers for BA are currently unavailable. In this study, we discovered potential biomarkers for BA using deep proteome analysis by data-independent acquisition mass spectrometry (DIA–MS). Four patients with BA and three patients with neonatal cholestasis of other etiologies (non-BA) were recruited for stool proteome analysis. Among the 2110 host-derived proteins detected in their stools, 49 proteins were significantly higher in patients with BA and 54 proteins were significantly lower. These varying stool protein levels in infants with BA can provide potential biomarkers for BA. As demonstrated in this study, the deep proteome analysis of stools has great potential not only in detecting new stool biomarkers for BA but also in elucidating the pathophysiology of BA and other pediatric diseases, especially in the field of pediatric gastroenterology. |
topic |
biliary atresia proteome analysis DIA–MS stool biomarker |
url |
https://www.mdpi.com/2227-7382/8/4/36 |
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