Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components

To investigate the role of basement membrane (BM) in chitosan-mediated morphogenesis of the salivary glands, the embryonic submandibular gland (SMG) experimental model was used. Chitosan promotes branching at distinct stages in SMG morphogenesis. When enzymes such as type IV collagenase, dispase, an...

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Main Authors: Ya-Chuan Hsiao, Tsung-Lin Yang
Format: Article
Language:English
Published: Elsevier 2015-09-01
Series:Data in Brief
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340915001250
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spelling doaj-fb2fce92b09c4d39930ce245621c7ea92020-11-25T01:29:30ZengElsevierData in Brief2352-34092015-09-014C55155810.1016/j.dib.2015.07.006Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane componentsYa-Chuan Hsiao0Tsung-Lin Yang1Department of Otolaryngology, National Taiwan University Hospital and College of Medicine, Taipei, TaiwanDepartment of Otolaryngology, National Taiwan University Hospital and College of Medicine, Taipei, TaiwanTo investigate the role of basement membrane (BM) in chitosan-mediated morphogenesis of the salivary glands, the embryonic submandibular gland (SMG) experimental model was used. Chitosan promotes branching at distinct stages in SMG morphogenesis. When enzymes such as type IV collagenase, dispase, and cathepsin B were used to digest the BM components, the morphogenetic effect mediated by chitosan disappeared. Immunofluorescence revealed that the corresponding receptors for BM components, including CD49c, CD49f, CD29, and dystroglycan, were locally enriched at the epithelial–mesenchymal junction around BM areas. The functional roles of laminin α1 and α5 in SMG branching were explored via siRNA knockdown, and suppression was confirmed at both the RNA and protein levels (Yang and Hsiao, Biomaterials, http://dx.doi.org/10.1016/j.biomaterials.2015.06.028, 2015). This data article demonstrates the experimental approaches to investigate the role of basement membrane in the structure formation of the salivary gland engineered by biomaterials.http://www.sciencedirect.com/science/article/pii/S2352340915001250Salivary glandsChitosanBasement membraneMorphogenesis
collection DOAJ
language English
format Article
sources DOAJ
author Ya-Chuan Hsiao
Tsung-Lin Yang
spellingShingle Ya-Chuan Hsiao
Tsung-Lin Yang
Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components
Data in Brief
Salivary glands
Chitosan
Basement membrane
Morphogenesis
author_facet Ya-Chuan Hsiao
Tsung-Lin Yang
author_sort Ya-Chuan Hsiao
title Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components
title_short Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components
title_full Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components
title_fullStr Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components
title_full_unstemmed Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components
title_sort data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2015-09-01
description To investigate the role of basement membrane (BM) in chitosan-mediated morphogenesis of the salivary glands, the embryonic submandibular gland (SMG) experimental model was used. Chitosan promotes branching at distinct stages in SMG morphogenesis. When enzymes such as type IV collagenase, dispase, and cathepsin B were used to digest the BM components, the morphogenetic effect mediated by chitosan disappeared. Immunofluorescence revealed that the corresponding receptors for BM components, including CD49c, CD49f, CD29, and dystroglycan, were locally enriched at the epithelial–mesenchymal junction around BM areas. The functional roles of laminin α1 and α5 in SMG branching were explored via siRNA knockdown, and suppression was confirmed at both the RNA and protein levels (Yang and Hsiao, Biomaterials, http://dx.doi.org/10.1016/j.biomaterials.2015.06.028, 2015). This data article demonstrates the experimental approaches to investigate the role of basement membrane in the structure formation of the salivary gland engineered by biomaterials.
topic Salivary glands
Chitosan
Basement membrane
Morphogenesis
url http://www.sciencedirect.com/science/article/pii/S2352340915001250
work_keys_str_mv AT yachuanhsiao datasupportingchitosanfacilitatesstructureformationofthesalivaryglandbyregulatingthebasementmembranecomponents
AT tsunglinyang datasupportingchitosanfacilitatesstructureformationofthesalivaryglandbyregulatingthebasementmembranecomponents
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