Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components
To investigate the role of basement membrane (BM) in chitosan-mediated morphogenesis of the salivary glands, the embryonic submandibular gland (SMG) experimental model was used. Chitosan promotes branching at distinct stages in SMG morphogenesis. When enzymes such as type IV collagenase, dispase, an...
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doaj-fb2fce92b09c4d39930ce245621c7ea92020-11-25T01:29:30ZengElsevierData in Brief2352-34092015-09-014C55155810.1016/j.dib.2015.07.006Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane componentsYa-Chuan Hsiao0Tsung-Lin Yang1Department of Otolaryngology, National Taiwan University Hospital and College of Medicine, Taipei, TaiwanDepartment of Otolaryngology, National Taiwan University Hospital and College of Medicine, Taipei, TaiwanTo investigate the role of basement membrane (BM) in chitosan-mediated morphogenesis of the salivary glands, the embryonic submandibular gland (SMG) experimental model was used. Chitosan promotes branching at distinct stages in SMG morphogenesis. When enzymes such as type IV collagenase, dispase, and cathepsin B were used to digest the BM components, the morphogenetic effect mediated by chitosan disappeared. Immunofluorescence revealed that the corresponding receptors for BM components, including CD49c, CD49f, CD29, and dystroglycan, were locally enriched at the epithelial–mesenchymal junction around BM areas. The functional roles of laminin α1 and α5 in SMG branching were explored via siRNA knockdown, and suppression was confirmed at both the RNA and protein levels (Yang and Hsiao, Biomaterials, http://dx.doi.org/10.1016/j.biomaterials.2015.06.028, 2015). This data article demonstrates the experimental approaches to investigate the role of basement membrane in the structure formation of the salivary gland engineered by biomaterials.http://www.sciencedirect.com/science/article/pii/S2352340915001250Salivary glandsChitosanBasement membraneMorphogenesis |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ya-Chuan Hsiao Tsung-Lin Yang |
spellingShingle |
Ya-Chuan Hsiao Tsung-Lin Yang Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components Data in Brief Salivary glands Chitosan Basement membrane Morphogenesis |
author_facet |
Ya-Chuan Hsiao Tsung-Lin Yang |
author_sort |
Ya-Chuan Hsiao |
title |
Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components |
title_short |
Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components |
title_full |
Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components |
title_fullStr |
Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components |
title_full_unstemmed |
Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components |
title_sort |
data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components |
publisher |
Elsevier |
series |
Data in Brief |
issn |
2352-3409 |
publishDate |
2015-09-01 |
description |
To investigate the role of basement membrane (BM) in chitosan-mediated morphogenesis of the salivary glands, the embryonic submandibular gland (SMG) experimental model was used. Chitosan promotes branching at distinct stages in SMG morphogenesis. When enzymes such as type IV collagenase, dispase, and cathepsin B were used to digest the BM components, the morphogenetic effect mediated by chitosan disappeared. Immunofluorescence revealed that the corresponding receptors for BM components, including CD49c, CD49f, CD29, and dystroglycan, were locally enriched at the epithelial–mesenchymal junction around BM areas. The functional roles of laminin α1 and α5 in SMG branching were explored via siRNA knockdown, and suppression was confirmed at both the RNA and protein levels (Yang and Hsiao, Biomaterials, http://dx.doi.org/10.1016/j.biomaterials.2015.06.028, 2015). This data article demonstrates the experimental approaches to investigate the role of basement membrane in the structure formation of the salivary gland engineered by biomaterials. |
topic |
Salivary glands Chitosan Basement membrane Morphogenesis |
url |
http://www.sciencedirect.com/science/article/pii/S2352340915001250 |
work_keys_str_mv |
AT yachuanhsiao datasupportingchitosanfacilitatesstructureformationofthesalivaryglandbyregulatingthebasementmembranecomponents AT tsunglinyang datasupportingchitosanfacilitatesstructureformationofthesalivaryglandbyregulatingthebasementmembranecomponents |
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1725096690411634688 |