Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide

Separation of short single- and double-stranded DNA typically requires gel electrophoresis followed by DNA extraction, which is a time consuming process. Graphene oxide adsorbs single-stranded DNA more quickly than double-stranded ones, allowing for selective removal of the former with a simple mixi...

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Main Authors:	Po-Jung Jimmy Huang, Juewen Liu
Format:	Article
Language:	English
Published:	MDPI AG 2013-04-01
Series:	Nanomaterials
Subjects:	graphene DNA adsorption fluorescence separation gel electrophoresis
Online Access:	http://www.mdpi.com/2079-4991/3/2/221

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record_format	Article
spelling	doaj-fb399bb064f44b5f8eee1c3047a463a42020-11-24T23:52:32ZengMDPI AGNanomaterials2079-49912013-04-013222122810.3390/nano3020221Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene OxidePo-Jung Jimmy HuangJuewen LiuSeparation of short single- and double-stranded DNA typically requires gel electrophoresis followed by DNA extraction, which is a time consuming process. Graphene oxide adsorbs single-stranded DNA more quickly than double-stranded ones, allowing for selective removal of the former with a simple mixing and centrifugation operation. The effect of DNA length and salt on adsorption selectivity has been characterized and its application in DNA melting curve measurement has been demonstrated.http://www.mdpi.com/2079-4991/3/2/221grapheneDNAadsorptionfluorescenceseparationgel electrophoresis
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Po-Jung Jimmy Huang Juewen Liu
spellingShingle	Po-Jung Jimmy Huang Juewen Liu Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide Nanomaterials graphene DNA adsorption fluorescence separation gel electrophoresis
author_facet	Po-Jung Jimmy Huang Juewen Liu
author_sort	Po-Jung Jimmy Huang
title	Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide
title_short	Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide
title_full	Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide
title_fullStr	Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide
title_full_unstemmed	Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide
title_sort	separation of short single- and double-stranded dna based on their adsorption kinetics difference on graphene oxide
publisher	MDPI AG
series	Nanomaterials
issn	2079-4991
publishDate	2013-04-01
description	Separation of short single- and double-stranded DNA typically requires gel electrophoresis followed by DNA extraction, which is a time consuming process. Graphene oxide adsorbs single-stranded DNA more quickly than double-stranded ones, allowing for selective removal of the former with a simple mixing and centrifugation operation. The effect of DNA length and salt on adsorption selectivity has been characterized and its application in DNA melting curve measurement has been demonstrated.
topic	graphene DNA adsorption fluorescence separation gel electrophoresis
url	http://www.mdpi.com/2079-4991/3/2/221
work_keys_str_mv	AT pojungjimmyhuang separationofshortsingleanddoublestrandeddnabasedontheiradsorptionkineticsdifferenceongrapheneoxide AT juewenliu separationofshortsingleanddoublestrandeddnabasedontheiradsorptionkineticsdifferenceongrapheneoxide
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Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide

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