Analysis of the Level of Plasmid-Derived mRNA in the Presence of Residual Plasmid DNA by Two-Step Quantitative RT-PCR
In transfection experiments with mammalian cells aiming to overexpress a specific protein, it is often necessary to correctly quantify the level of the recombinant and the corresponding endogenous mRNA. In our case, mouse calvarial osteoblasts were transfected with a vector containing the complete &...
Main Authors: | Barbara Ahlemeyer, Claudia Colasante, Eveline Baumgart-Vogt |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-05-01
|
Series: | Methods and Protocols |
Subjects: | |
Online Access: | https://www.mdpi.com/2409-9279/3/2/40 |
Similar Items
-
Mechanisms and Regulation of Nonsense-Mediated mRNA Decay and Nonsense-Associated Altered Splicing in Lymphocytes
by: Jean-Marie Lambert, et al.
Published: (2020-02-01) -
Nonsense suppression induced readthrough of a novel PAX6 mutation in patient‐derived cells of congenital aniridia
by: Xiaoliang Liu, et al.
Published: (2020-05-01) -
A nonsense variant in FBN1 caused autosomal dominant Marfan syndrome in a Chinese family: a case report
by: Yuping Niu, et al.
Published: (2020-10-01) -
The Effect of SF3B1 Mutation on the DNA Damage Response and Nonsense-Mediated mRNA Decay in Cancer
by: Alexander C. Leeksma, et al.
Published: (2021-01-01) -
Stop that nonsense!
by: Catherine L Jopling
Published: (2014-09-01)