Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments
Antibiotic resistance has become one of the world’s most severe problems because of the overuse of antibiotics. Antibiotic-resistant bacteria are more difficult to kill and more expensive to treat. Researchers have been studied on antibiotic alternatives such as antimicrobial peptides and lipopeptid...
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doaj-fb837081ea0c464fa4f523a6c622b87a2020-11-24T21:08:56ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-05-01810.3389/fmicb.2017.00871256331Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine SedimentsYulin Chen0Yulin Chen1Shiliang A. Liu2Haijin Mou3Yunxiao Ma4Meng Li5Xiaoke Hu6Yantai Institute of Coastal Zone Research, Chinese Academy of SciencesYantai, ChinaSchool of Food Science and Engineering, Ocean University of ChinaQingdao, ChinaSchool of Veterinary Medicine, Louisiana State University, Baton RougeLA, USASchool of Food Science and Engineering, Ocean University of ChinaQingdao, ChinaSchool of Food Science and Engineering, Ocean University of ChinaQingdao, ChinaYantai Institute of Coastal Zone Research, Chinese Academy of SciencesYantai, ChinaYantai Institute of Coastal Zone Research, Chinese Academy of SciencesYantai, ChinaAntibiotic resistance has become one of the world’s most severe problems because of the overuse of antibiotics. Antibiotic-resistant bacteria are more difficult to kill and more expensive to treat. Researchers have been studied on antibiotic alternatives such as antimicrobial peptides and lipopeptides. A functional bacteria MB01 producing lipopeptides which can be used as bacteriostat was isolated from the Bohai Sea sediments, which had been identified as Bacillus licheniformis by the morphological, physiological, and biochemical identification and 16s rDNA sequence. The lipopeptides produced by MB01 were determined to be cyclic surfactin homologs by LC-ESI-MS structural identification after crude extraction and LH-20 chromatography. [M+H]+m/z 994, 1008, 1022, and 1036 were all the characteristic molecular weight of surfactin homologs. CID analysis revealed that the molecular structure of the lipopeptides was Rn-Glu1-Leu/Ile2-Leu3-Val4-Asp5-Leu6-Leu/Ile7. The lipopeptides showed well resistance to UV light and the change of pH and temperature.http://journal.frontiersin.org/article/10.3389/fmicb.2017.00871/fullBacillus licheniformispurificationbacteriostatstructureLC-ESI-MS/MSstabilization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yulin Chen Yulin Chen Shiliang A. Liu Haijin Mou Yunxiao Ma Meng Li Xiaoke Hu |
spellingShingle |
Yulin Chen Yulin Chen Shiliang A. Liu Haijin Mou Yunxiao Ma Meng Li Xiaoke Hu Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments Frontiers in Microbiology Bacillus licheniformis purification bacteriostat structure LC-ESI-MS/MS stabilization |
author_facet |
Yulin Chen Yulin Chen Shiliang A. Liu Haijin Mou Yunxiao Ma Meng Li Xiaoke Hu |
author_sort |
Yulin Chen |
title |
Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments |
title_short |
Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments |
title_full |
Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments |
title_fullStr |
Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments |
title_full_unstemmed |
Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments |
title_sort |
characterization of lipopeptide biosurfactants produced by bacillus licheniformis mb01 from marine sediments |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2017-05-01 |
description |
Antibiotic resistance has become one of the world’s most severe problems because of the overuse of antibiotics. Antibiotic-resistant bacteria are more difficult to kill and more expensive to treat. Researchers have been studied on antibiotic alternatives such as antimicrobial peptides and lipopeptides. A functional bacteria MB01 producing lipopeptides which can be used as bacteriostat was isolated from the Bohai Sea sediments, which had been identified as Bacillus licheniformis by the morphological, physiological, and biochemical identification and 16s rDNA sequence. The lipopeptides produced by MB01 were determined to be cyclic surfactin homologs by LC-ESI-MS structural identification after crude extraction and LH-20 chromatography. [M+H]+m/z 994, 1008, 1022, and 1036 were all the characteristic molecular weight of surfactin homologs. CID analysis revealed that the molecular structure of the lipopeptides was Rn-Glu1-Leu/Ile2-Leu3-Val4-Asp5-Leu6-Leu/Ile7. The lipopeptides showed well resistance to UV light and the change of pH and temperature. |
topic |
Bacillus licheniformis purification bacteriostat structure LC-ESI-MS/MS stabilization |
url |
http://journal.frontiersin.org/article/10.3389/fmicb.2017.00871/full |
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