Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions

Traction force microscopy is an effective method for measuring cellular forces but it is limited by planar geometry. Here the authors develop a facile method to produce deformable hydrogel particles and a reference-free computational method to resolve surface traction forces from particle shape defo...

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Main Authors:	Daan Vorselen, Yifan Wang, Miguel M. de Jesus, Pavak K. Shah, Matthew J. Footer, Morgan Huse, Wei Cai, Julie A. Theriot
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-01-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-019-13804-z

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spelling	doaj-fbc3f6afb17f46f49e6de46d02f86e782021-05-11T09:16:33ZengNature Publishing GroupNature Communications2041-17232020-01-0111111410.1038/s41467-019-13804-zMicroparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactionsDaan Vorselen0Yifan Wang1Miguel M. de Jesus2Pavak K. Shah3Matthew J. Footer4Morgan Huse5Wei Cai6Julie A. Theriot7Department of Biochemistry, Stanford UniversityDepartment of Mechanical Engineering, Stanford UniversityImmunology Program, Memorial Sloan Kettering Cancer CenterDevelopmental Biology Program, Sloan Kettering InstituteDepartment of Biochemistry, Stanford UniversityImmunology Program, Memorial Sloan Kettering Cancer CenterDepartment of Mechanical Engineering, Stanford UniversityDepartment of Biochemistry, Stanford UniversityTraction force microscopy is an effective method for measuring cellular forces but it is limited by planar geometry. Here the authors develop a facile method to produce deformable hydrogel particles and a reference-free computational method to resolve surface traction forces from particle shape deformation.https://doi.org/10.1038/s41467-019-13804-z
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Daan Vorselen Yifan Wang Miguel M. de Jesus Pavak K. Shah Matthew J. Footer Morgan Huse Wei Cai Julie A. Theriot
spellingShingle	Daan Vorselen Yifan Wang Miguel M. de Jesus Pavak K. Shah Matthew J. Footer Morgan Huse Wei Cai Julie A. Theriot Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions Nature Communications
author_facet	Daan Vorselen Yifan Wang Miguel M. de Jesus Pavak K. Shah Matthew J. Footer Morgan Huse Wei Cai Julie A. Theriot
author_sort	Daan Vorselen
title	Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions
title_short	Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions
title_full	Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions
title_fullStr	Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions
title_full_unstemmed	Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions
title_sort	microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-01-01
description	Traction force microscopy is an effective method for measuring cellular forces but it is limited by planar geometry. Here the authors develop a facile method to produce deformable hydrogel particles and a reference-free computational method to resolve surface traction forces from particle shape deformation.
url	https://doi.org/10.1038/s41467-019-13804-z
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Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions

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