Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions
Traction force microscopy is an effective method for measuring cellular forces but it is limited by planar geometry. Here the authors develop a facile method to produce deformable hydrogel particles and a reference-free computational method to resolve surface traction forces from particle shape defo...
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2020-01-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-019-13804-z |
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doaj-fbc3f6afb17f46f49e6de46d02f86e782021-05-11T09:16:33ZengNature Publishing GroupNature Communications2041-17232020-01-0111111410.1038/s41467-019-13804-zMicroparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactionsDaan Vorselen0Yifan Wang1Miguel M. de Jesus2Pavak K. Shah3Matthew J. Footer4Morgan Huse5Wei Cai6Julie A. Theriot7Department of Biochemistry, Stanford UniversityDepartment of Mechanical Engineering, Stanford UniversityImmunology Program, Memorial Sloan Kettering Cancer CenterDevelopmental Biology Program, Sloan Kettering InstituteDepartment of Biochemistry, Stanford UniversityImmunology Program, Memorial Sloan Kettering Cancer CenterDepartment of Mechanical Engineering, Stanford UniversityDepartment of Biochemistry, Stanford UniversityTraction force microscopy is an effective method for measuring cellular forces but it is limited by planar geometry. Here the authors develop a facile method to produce deformable hydrogel particles and a reference-free computational method to resolve surface traction forces from particle shape deformation.https://doi.org/10.1038/s41467-019-13804-z |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daan Vorselen Yifan Wang Miguel M. de Jesus Pavak K. Shah Matthew J. Footer Morgan Huse Wei Cai Julie A. Theriot |
spellingShingle |
Daan Vorselen Yifan Wang Miguel M. de Jesus Pavak K. Shah Matthew J. Footer Morgan Huse Wei Cai Julie A. Theriot Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions Nature Communications |
author_facet |
Daan Vorselen Yifan Wang Miguel M. de Jesus Pavak K. Shah Matthew J. Footer Morgan Huse Wei Cai Julie A. Theriot |
author_sort |
Daan Vorselen |
title |
Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions |
title_short |
Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions |
title_full |
Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions |
title_fullStr |
Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions |
title_full_unstemmed |
Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions |
title_sort |
microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions |
publisher |
Nature Publishing Group |
series |
Nature Communications |
issn |
2041-1723 |
publishDate |
2020-01-01 |
description |
Traction force microscopy is an effective method for measuring cellular forces but it is limited by planar geometry. Here the authors develop a facile method to produce deformable hydrogel particles and a reference-free computational method to resolve surface traction forces from particle shape deformation. |
url |
https://doi.org/10.1038/s41467-019-13804-z |
work_keys_str_mv |
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