Proteomic analysis in the Dufour's gland of Africanized Apis mellifera workers (Hymenoptera: Apidae).
The colony of eusocial bee Apis mellifera has a reproductive queen and sterile workers performing tasks such as brood care and foraging. Chemical communication plays a crucial role in the maintenance of sociability in bees with many compounds released by the exocrine glands. The Dufour's gland...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2017-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC5443511?pdf=render |
id |
doaj-fbe443e670bb48e38266eeaa9f289e0c |
---|---|
record_format |
Article |
spelling |
doaj-fbe443e670bb48e38266eeaa9f289e0c2020-11-25T00:06:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01125e017741510.1371/journal.pone.0177415Proteomic analysis in the Dufour's gland of Africanized Apis mellifera workers (Hymenoptera: Apidae).Aparecida das Dores TeixeiraPatricia D GamesBenjamin B KatzJohn M TomichJosé C ZanuncioJosé Eduardo SerrãoThe colony of eusocial bee Apis mellifera has a reproductive queen and sterile workers performing tasks such as brood care and foraging. Chemical communication plays a crucial role in the maintenance of sociability in bees with many compounds released by the exocrine glands. The Dufour's gland is a non-paired gland associated with the sting apparatus with important functions in the communication between members of the colony, releasing volatile chemicals that influence workers roles and tasks. However, the protein content in this gland is not well studied. This study identified differentially expressed proteins in the Dufour's glands of nurse and forager workers of A. mellifera through 2D-gel electrophoresis and mass spectrometry. A total of 131 spots showed different expression between nurse and forager bees, and 28 proteins were identified. The identified proteins were categorized into different functions groups including protein, carbohydrate, energy and lipid metabolisms, cytoskeleton-associated proteins, detoxification, homeostasis, cell communication, constitutive and allergen. This study provides new insights of the protein content in the Dufour's gland contributing to a more complete understanding of the biological functions of this gland in honeybees.http://europepmc.org/articles/PMC5443511?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aparecida das Dores Teixeira Patricia D Games Benjamin B Katz John M Tomich José C Zanuncio José Eduardo Serrão |
spellingShingle |
Aparecida das Dores Teixeira Patricia D Games Benjamin B Katz John M Tomich José C Zanuncio José Eduardo Serrão Proteomic analysis in the Dufour's gland of Africanized Apis mellifera workers (Hymenoptera: Apidae). PLoS ONE |
author_facet |
Aparecida das Dores Teixeira Patricia D Games Benjamin B Katz John M Tomich José C Zanuncio José Eduardo Serrão |
author_sort |
Aparecida das Dores Teixeira |
title |
Proteomic analysis in the Dufour's gland of Africanized Apis mellifera workers (Hymenoptera: Apidae). |
title_short |
Proteomic analysis in the Dufour's gland of Africanized Apis mellifera workers (Hymenoptera: Apidae). |
title_full |
Proteomic analysis in the Dufour's gland of Africanized Apis mellifera workers (Hymenoptera: Apidae). |
title_fullStr |
Proteomic analysis in the Dufour's gland of Africanized Apis mellifera workers (Hymenoptera: Apidae). |
title_full_unstemmed |
Proteomic analysis in the Dufour's gland of Africanized Apis mellifera workers (Hymenoptera: Apidae). |
title_sort |
proteomic analysis in the dufour's gland of africanized apis mellifera workers (hymenoptera: apidae). |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
The colony of eusocial bee Apis mellifera has a reproductive queen and sterile workers performing tasks such as brood care and foraging. Chemical communication plays a crucial role in the maintenance of sociability in bees with many compounds released by the exocrine glands. The Dufour's gland is a non-paired gland associated with the sting apparatus with important functions in the communication between members of the colony, releasing volatile chemicals that influence workers roles and tasks. However, the protein content in this gland is not well studied. This study identified differentially expressed proteins in the Dufour's glands of nurse and forager workers of A. mellifera through 2D-gel electrophoresis and mass spectrometry. A total of 131 spots showed different expression between nurse and forager bees, and 28 proteins were identified. The identified proteins were categorized into different functions groups including protein, carbohydrate, energy and lipid metabolisms, cytoskeleton-associated proteins, detoxification, homeostasis, cell communication, constitutive and allergen. This study provides new insights of the protein content in the Dufour's gland contributing to a more complete understanding of the biological functions of this gland in honeybees. |
url |
http://europepmc.org/articles/PMC5443511?pdf=render |
work_keys_str_mv |
AT aparecidadasdoresteixeira proteomicanalysisinthedufoursglandofafricanizedapismelliferaworkershymenopteraapidae AT patriciadgames proteomicanalysisinthedufoursglandofafricanizedapismelliferaworkershymenopteraapidae AT benjaminbkatz proteomicanalysisinthedufoursglandofafricanizedapismelliferaworkershymenopteraapidae AT johnmtomich proteomicanalysisinthedufoursglandofafricanizedapismelliferaworkershymenopteraapidae AT joseczanuncio proteomicanalysisinthedufoursglandofafricanizedapismelliferaworkershymenopteraapidae AT joseeduardoserrao proteomicanalysisinthedufoursglandofafricanizedapismelliferaworkershymenopteraapidae |
_version_ |
1725422328906514432 |