Optimization of isolation and transfection conditions of maize endosperm protoplasts

Optimization of isolation and transfection conditions of maize endosperm protoplasts

Abstract Background Endosperm-trait related genes are associated with grain yield or quality in maize. There are vast numbers of these genes whose functions and regulations are still unknown. The biolistic system, which is often used for transient gene expression, is expensive and involves complex p...

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Bibliographic Details
Main Authors: Yufeng Hu, Dalin Song, Lei Gao, Babatope Samuel Ajayo, Yongbin Wang, Huanhuan Huang, Junjie Zhang, Hanmei Liu, Yinghong Liu, Guowu Yu, Yongjian Liu, Yangping Li, Yubi Huang
Format: Article
Language:English
Published: BMC 2020-07-01
Series:Plant Methods
Subjects:
Endosperm
Genes’ functions
Maize
Protoplast
Response surface
Transient system
Online Access:http://link.springer.com/article/10.1186/s13007-020-00636-y

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