Intracellular <i>Staphylococcus aureus</i> Elicits the Production of Host Very Long-Chain Saturated Fatty Acids with Antimicrobial Activity
As a facultative intracellular pathogen, <i>Staphylococcus aureus</i> is able to invade and proliferate within many types of mammalian cells. Intracellular bacterial replication relies on host nutrient supplies and, therefore, cell metabolism is closely bound to intracellular infection....
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doaj-fbe8f443c9b545bbb8c865ee7fceb0c42020-11-25T01:18:27ZengMDPI AGMetabolites2218-19892019-07-019714810.3390/metabo9070148metabo9070148Intracellular <i>Staphylococcus aureus</i> Elicits the Production of Host Very Long-Chain Saturated Fatty Acids with Antimicrobial ActivityNatalia Bravo-Santano0James K. Ellis1Yolanda Calle2Hector C. Keun3Volker Behrends4Michal Letek5Health Sciences Research Centre, University of Roehampton, London SW15 4JD, UKDivision of Cancer, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London W12 0HS, UKHealth Sciences Research Centre, University of Roehampton, London SW15 4JD, UKDivision of Cancer, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London W12 0HS, UKHealth Sciences Research Centre, University of Roehampton, London SW15 4JD, UKHealth Sciences Research Centre, University of Roehampton, London SW15 4JD, UKAs a facultative intracellular pathogen, <i>Staphylococcus aureus</i> is able to invade and proliferate within many types of mammalian cells. Intracellular bacterial replication relies on host nutrient supplies and, therefore, cell metabolism is closely bound to intracellular infection. Here, we investigated how <i>S. aureus</i> invasion affects the host membrane-bound fatty acids. We quantified the relative levels of fatty acids and their labelling pattern after intracellular infection by gas chromatography-mass spectrometry (GC-MS). Interestingly, we observed that the levels of three host fatty acids—docosanoic, eicosanoic and palmitic acids—were significantly increased in response to intracellular <i>S. aureus</i> infection. Accordingly, labelling carbon distribution was also affected in infected cells, in comparison to the uninfected control. In addition, treatment of HeLa cells with these three fatty acids showed a cytoprotective role by directly reducing <i>S. aureus</i> growth.https://www.mdpi.com/2218-1989/9/7/148intracellular infectionlipids metabolismfatty acidsantibacterial effect<i>Staphylococcus aureus</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Natalia Bravo-Santano James K. Ellis Yolanda Calle Hector C. Keun Volker Behrends Michal Letek |
spellingShingle |
Natalia Bravo-Santano James K. Ellis Yolanda Calle Hector C. Keun Volker Behrends Michal Letek Intracellular <i>Staphylococcus aureus</i> Elicits the Production of Host Very Long-Chain Saturated Fatty Acids with Antimicrobial Activity Metabolites intracellular infection lipids metabolism fatty acids antibacterial effect <i>Staphylococcus aureus</i> |
author_facet |
Natalia Bravo-Santano James K. Ellis Yolanda Calle Hector C. Keun Volker Behrends Michal Letek |
author_sort |
Natalia Bravo-Santano |
title |
Intracellular <i>Staphylococcus aureus</i> Elicits the Production of Host Very Long-Chain Saturated Fatty Acids with Antimicrobial Activity |
title_short |
Intracellular <i>Staphylococcus aureus</i> Elicits the Production of Host Very Long-Chain Saturated Fatty Acids with Antimicrobial Activity |
title_full |
Intracellular <i>Staphylococcus aureus</i> Elicits the Production of Host Very Long-Chain Saturated Fatty Acids with Antimicrobial Activity |
title_fullStr |
Intracellular <i>Staphylococcus aureus</i> Elicits the Production of Host Very Long-Chain Saturated Fatty Acids with Antimicrobial Activity |
title_full_unstemmed |
Intracellular <i>Staphylococcus aureus</i> Elicits the Production of Host Very Long-Chain Saturated Fatty Acids with Antimicrobial Activity |
title_sort |
intracellular <i>staphylococcus aureus</i> elicits the production of host very long-chain saturated fatty acids with antimicrobial activity |
publisher |
MDPI AG |
series |
Metabolites |
issn |
2218-1989 |
publishDate |
2019-07-01 |
description |
As a facultative intracellular pathogen, <i>Staphylococcus aureus</i> is able to invade and proliferate within many types of mammalian cells. Intracellular bacterial replication relies on host nutrient supplies and, therefore, cell metabolism is closely bound to intracellular infection. Here, we investigated how <i>S. aureus</i> invasion affects the host membrane-bound fatty acids. We quantified the relative levels of fatty acids and their labelling pattern after intracellular infection by gas chromatography-mass spectrometry (GC-MS). Interestingly, we observed that the levels of three host fatty acids—docosanoic, eicosanoic and palmitic acids—were significantly increased in response to intracellular <i>S. aureus</i> infection. Accordingly, labelling carbon distribution was also affected in infected cells, in comparison to the uninfected control. In addition, treatment of HeLa cells with these three fatty acids showed a cytoprotective role by directly reducing <i>S. aureus</i> growth. |
topic |
intracellular infection lipids metabolism fatty acids antibacterial effect <i>Staphylococcus aureus</i> |
url |
https://www.mdpi.com/2218-1989/9/7/148 |
work_keys_str_mv |
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