Probing compartment-specific sphingolipids with targeted bacterial sphingomyelinases and ceramidases
Sphingolipids contribute to the regulation of cell and tissue homeostasis, and disorders of sphingolipid metabolism lead to diseases such as inflammation, stroke, diabetes, and cancer. Sphingolipid metabolic pathways involve an array of enzymes that reside in specific subcellular organelles, resulti...
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doaj-fbfa3d5b139a421db62850115a86db7d2021-04-29T04:35:28ZengElsevierJournal of Lipid Research0022-22752019-11-01601118411850Probing compartment-specific sphingolipids with targeted bacterial sphingomyelinases and ceramidasesWataru Sakamoto0Daniel Canals1Silvia Salamone2Janet Allopenna3Christopher J. Clarke4Justin Snider5Lina M. Obeid6Yusuf A. Hannun7Department of Medicine and Stony Brook Cancer Center, Stony Brook University, Stony Brook, NY; Ono Pharmaceutical Company, Ltd. Oncology Research Laboratories, Osaka, JapanDepartment of Medicine and Stony Brook Cancer Center, Stony Brook University, Stony Brook, NYDepartment of Medicine and Stony Brook Cancer Center, Stony Brook University, Stony Brook, NYDepartment of Medicine and Stony Brook Cancer Center, Stony Brook University, Stony Brook, NYDepartment of Medicine and Stony Brook Cancer Center, Stony Brook University, Stony Brook, NYDepartment of Medicine and Stony Brook Cancer Center, Stony Brook University, Stony Brook, NYDepartment of Medicine and Stony Brook Cancer Center, Stony Brook University, Stony Brook, NY; Northport Veterans Affairs Medical Center, Northport, NYDepartment of Medicine and Stony Brook Cancer Center, Stony Brook University, Stony Brook, NY; Departments of Biochemistry, Pharmacology, and Pathology, Stony Brook University, Stony Brook, NY; To whom correspondence should be addressed.Sphingolipids contribute to the regulation of cell and tissue homeostasis, and disorders of sphingolipid metabolism lead to diseases such as inflammation, stroke, diabetes, and cancer. Sphingolipid metabolic pathways involve an array of enzymes that reside in specific subcellular organelles, resulting in the formation of many diverse sphingolipids with distinct molecular species based on the diversity of the ceramide (Cer) structure. In order to probe compartment-specific metabolism of sphingolipids in this study, we analyzed the Cer and SM species preferentially produced in the inner plasma membrane (PM), Golgi apparatus, ER, mitochondria, nucleus, and cytoplasm by using compartmentally targeted bacterial SMases and ceramidases. The results showed that the length of the acyl chain of Cer becomes longer according to the progress of Cer from synthesis in the ER to the Golgi apparatus, then to the PM. These findings suggest that each organelle shows different properties of SM-derived Cers consistent with its emerging distinct functions in vitro and in vivo.http://www.sciencedirect.com/science/article/pii/S0022227520322835ceramide molecular speciessphingosinesphingomyelinmass spectrometrycellular organelles |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wataru Sakamoto Daniel Canals Silvia Salamone Janet Allopenna Christopher J. Clarke Justin Snider Lina M. Obeid Yusuf A. Hannun |
spellingShingle |
Wataru Sakamoto Daniel Canals Silvia Salamone Janet Allopenna Christopher J. Clarke Justin Snider Lina M. Obeid Yusuf A. Hannun Probing compartment-specific sphingolipids with targeted bacterial sphingomyelinases and ceramidases Journal of Lipid Research ceramide molecular species sphingosine sphingomyelin mass spectrometry cellular organelles |
author_facet |
Wataru Sakamoto Daniel Canals Silvia Salamone Janet Allopenna Christopher J. Clarke Justin Snider Lina M. Obeid Yusuf A. Hannun |
author_sort |
Wataru Sakamoto |
title |
Probing compartment-specific sphingolipids with targeted bacterial sphingomyelinases and ceramidases |
title_short |
Probing compartment-specific sphingolipids with targeted bacterial sphingomyelinases and ceramidases |
title_full |
Probing compartment-specific sphingolipids with targeted bacterial sphingomyelinases and ceramidases |
title_fullStr |
Probing compartment-specific sphingolipids with targeted bacterial sphingomyelinases and ceramidases |
title_full_unstemmed |
Probing compartment-specific sphingolipids with targeted bacterial sphingomyelinases and ceramidases |
title_sort |
probing compartment-specific sphingolipids with targeted bacterial sphingomyelinases and ceramidases |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2019-11-01 |
description |
Sphingolipids contribute to the regulation of cell and tissue homeostasis, and disorders of sphingolipid metabolism lead to diseases such as inflammation, stroke, diabetes, and cancer. Sphingolipid metabolic pathways involve an array of enzymes that reside in specific subcellular organelles, resulting in the formation of many diverse sphingolipids with distinct molecular species based on the diversity of the ceramide (Cer) structure. In order to probe compartment-specific metabolism of sphingolipids in this study, we analyzed the Cer and SM species preferentially produced in the inner plasma membrane (PM), Golgi apparatus, ER, mitochondria, nucleus, and cytoplasm by using compartmentally targeted bacterial SMases and ceramidases. The results showed that the length of the acyl chain of Cer becomes longer according to the progress of Cer from synthesis in the ER to the Golgi apparatus, then to the PM. These findings suggest that each organelle shows different properties of SM-derived Cers consistent with its emerging distinct functions in vitro and in vivo. |
topic |
ceramide molecular species sphingosine sphingomyelin mass spectrometry cellular organelles |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520322835 |
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