Investigations into the presence of functional ß1, ß2 and ß3-adrenoceptors in urothelium and detrusor of human bladder
Purpose: We investigated the presence of functional ß1, ß2 and ß3-adrenoceptor in urothelium and detrusor muscle of human bladder through in vitro pharmacology of selective ß3 adrenoceptor agonist solabegron. Materials and Methods: Expression of these adrenoceptors in surgically separated human urot...
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Sociedade Brasileira de Urologia
2009-02-01
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doaj-fc1dcae948ec4bb0bfaee2e86762d9a92020-11-24T23:13:16ZengSociedade Brasileira de UrologiaInternational Brazilian Journal of Urology1677-55381677-61192009-02-01351768310.1590/S1677-55382009000100012Investigations into the presence of functional ß1, ß2 and ß3-adrenoceptors in urothelium and detrusor of human bladderPradeep TyagiCatherine A. ThomasNaoki YoshimuraMichael B. ChancellorPurpose: We investigated the presence of functional ß1, ß2 and ß3-adrenoceptor in urothelium and detrusor muscle of human bladder through in vitro pharmacology of selective ß3 adrenoceptor agonist solabegron. Materials and Methods: Expression of these adrenoceptors in surgically separated human urothelium and detrusor muscle were investigated using RT-PCR. The effects of activating these receptors were studied by determining the relaxation produced by ß-adrenoceptors agonist in pre-contracted human detrusor strips. Results: The results confirmed the presence of mRNA for ß1, ß2 and ß3-adrenoceptor in both human urothelium and detrusor. In an in vitro functional bladder assay, Solabegron and other agonists for ß-adrenoceptors such as procaterol and isoproterenol evoked potent concentration-dependent relaxation of isolated human bladder strips with pD2 values of 8.73 ± 0.19, 5.08 ± 0.48 and 6.28 ± 0.54, respectively. Conclusions: Selective ß3-adrenoceptor agonist may be a potential new treatment for the overactive bladder OAB syndrome. Existence of ß3-adrenoceptor mRNA exists in the urothelium in addition to the detrusor muscle suggest multiple site of actions for the ß3-adrenoceptor in the lower urinary tract.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382009000100012bladderurotheliumdetrusoradrenoreceptors |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pradeep Tyagi Catherine A. Thomas Naoki Yoshimura Michael B. Chancellor |
spellingShingle |
Pradeep Tyagi Catherine A. Thomas Naoki Yoshimura Michael B. Chancellor Investigations into the presence of functional ß1, ß2 and ß3-adrenoceptors in urothelium and detrusor of human bladder International Brazilian Journal of Urology bladder urothelium detrusor adrenoreceptors |
author_facet |
Pradeep Tyagi Catherine A. Thomas Naoki Yoshimura Michael B. Chancellor |
author_sort |
Pradeep Tyagi |
title |
Investigations into the presence of functional ß1, ß2 and ß3-adrenoceptors in urothelium and detrusor of human bladder |
title_short |
Investigations into the presence of functional ß1, ß2 and ß3-adrenoceptors in urothelium and detrusor of human bladder |
title_full |
Investigations into the presence of functional ß1, ß2 and ß3-adrenoceptors in urothelium and detrusor of human bladder |
title_fullStr |
Investigations into the presence of functional ß1, ß2 and ß3-adrenoceptors in urothelium and detrusor of human bladder |
title_full_unstemmed |
Investigations into the presence of functional ß1, ß2 and ß3-adrenoceptors in urothelium and detrusor of human bladder |
title_sort |
investigations into the presence of functional ß1, ß2 and ß3-adrenoceptors in urothelium and detrusor of human bladder |
publisher |
Sociedade Brasileira de Urologia |
series |
International Brazilian Journal of Urology |
issn |
1677-5538 1677-6119 |
publishDate |
2009-02-01 |
description |
Purpose: We investigated the presence of functional ß1, ß2 and ß3-adrenoceptor in urothelium and detrusor muscle of human bladder through in vitro pharmacology of selective ß3 adrenoceptor agonist solabegron. Materials and Methods: Expression of these adrenoceptors in surgically separated human urothelium and detrusor muscle were investigated using RT-PCR. The effects of activating these receptors were studied by determining the relaxation produced by ß-adrenoceptors agonist in pre-contracted human detrusor strips. Results: The results confirmed the presence of mRNA for ß1, ß2 and ß3-adrenoceptor in both human urothelium and detrusor. In an in vitro functional bladder assay, Solabegron and other agonists for ß-adrenoceptors such as procaterol and isoproterenol evoked potent concentration-dependent relaxation of isolated human bladder strips with pD2 values of 8.73 ± 0.19, 5.08 ± 0.48 and 6.28 ± 0.54, respectively. Conclusions: Selective ß3-adrenoceptor agonist may be a potential new treatment for the overactive bladder OAB syndrome. Existence of ß3-adrenoceptor mRNA exists in the urothelium in addition to the detrusor muscle suggest multiple site of actions for the ß3-adrenoceptor in the lower urinary tract. |
topic |
bladder urothelium detrusor adrenoreceptors |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382009000100012 |
work_keys_str_mv |
AT pradeeptyagi investigationsintothepresenceoffunctionalß1ß2andß3adrenoceptorsinurotheliumanddetrusorofhumanbladder AT catherineathomas investigationsintothepresenceoffunctionalß1ß2andß3adrenoceptorsinurotheliumanddetrusorofhumanbladder AT naokiyoshimura investigationsintothepresenceoffunctionalß1ß2andß3adrenoceptorsinurotheliumanddetrusorofhumanbladder AT michaelbchancellor investigationsintothepresenceoffunctionalß1ß2andß3adrenoceptorsinurotheliumanddetrusorofhumanbladder |
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1725598651638611968 |