Diversity and frequency of resistance and virulence genes in blaKPC and blaNDM co-producing Klebsiella pneumoniae strains from China

• Main Authors: Liu X, Zhang J, Li Y, Shen Q, Jiang W, Zhao K, He Y, Dai P, Nie Z, Xu X, Zhou Y
• Format: Article
• Language: English
• Published: Dove Medical Press 2019-09-01
• Series: Infection and Drug Resistance
• Subjects: Klebsiella pneumoniae; blaNDM; blaKPC; Resistance genes; Virulence factors;
• Online Access: https://www.dovepress.com/diversity-and-frequency-of-resistance-and-virulence-genes-in-blakpc-an-peer-reviewed-article-IDR

Xin Liu,1,* Jie Zhang,1,* Yini Li,1 Qiuni Shen,1 Wenting Jiang,1 Kelei Zhao,2 Yancheng He,1 Penggao Dai,1 Zhihao Nie,1 Xiyan Xu,1,3 Yingshun Zhou1 1Department of Pathogenic Biology, School of Basic Medicine, Southwest Medical University, Luzhou, Sichuan, People’s Republic of China; 2Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, Sichuan 610052, People’s Republic of China; 3Department of Histology and Embryology, School of Basic Medicine, Southwest Medical University, Luzhou, Sichuan, People’s Republic of China*These authors contributed equally to this workCorrepsondence: Xiyan Xu; Yingshun ZhouDepartment of Pathogenic Biology, School of Basic Medicine, Southwest Medical University, No. 319, Zhongshan Road, Luzhou, Sichuan 646000, People’s Republic of ChinaTel [phone]Email [e-mail]: Emergence of blaKPC and blaNDM co-producing Klebsiella pneumoniae strains have led to the limited therapeutic options for clinical treatment. Understanding the diversity and frequency of resistance and virulence genes of these isolates is of great significance.Purpose: The aim of this study is to research the diversity and frequency of resistance and virulence genes in the blaKPC and blaNDM co-producing Klebsiella pneumoniae strains.Methods and Results: In this study, 117 K. pneumonia strains were isolated from China, and among of which, 24 were found to be blaKPC and blaNDM co-producing with significant resistance against almost all the commonly used antibiotics. Additionally, 4 strains were hypermucoviscous and 8 showed high serum resistance. Overall, blaSHV, blaCTX-M, tetA and sul1 resistance genes found in 100% of the isolates, followed by blaTEM (95.8%), oqxA/B (91.7%), qnrB (87.5%), aac(6’)Ib-cr (83.3%), blaDHA (79.2%), rmtB (66.7%), qnrS (54.2%), cat(54.2%), floR (50.0%), sul2 (45.8%) cmlA (20.8%)andblaCMY (8.33%), respectively. What’ more, seven blaCTX-M subtypes [blaCTX-M-14 (n=18), blaCTX-M-3(n=11), blaCTX-M-65 (n=4), blaCTX-M-15 (n=3), blaCTX-M-28 (n=2), blaCTX-M-55 (n=2), blaCTX-M-22 (n=1)] and six blaSHV subtypes [blaSHV-12(n=16), blaSHV-11 (n=4), blaSHV-2a(n=1), blaSHV-1(n=1), blaSHV-38(n=1) and blaSHV-28(n=1)] were detected. The frequency of virulence genes was as follows: 100% for entB, ybtS and irp, 95.8% for mrkD, 91.66% for fimH, 79.2% for iutA, 62.5% for iroBCDE, aerobactin and kfu, 66.7% for allS, 45.8% for wcaG, 37.5% for rmpA, 20.8% for pagO and 16.7% for magA.Conclusion: From this study, we concluded that the blaKPC and blaNDM co-producing Klebsiella pneumoniae strains have a high diversity and frequency of resistance and virulence genes. This study may offer hospitals important information about the control of infections caused by blaKPC and blaNDM co-producing Klebsiella pneumoniae.Keywords: Klebsiella pneumoniae, blaNDM, blaKPC, resistance genes, virulence factors