Roles of the Brassica napus DELLA Protein BnaA6.RGA, in Modulating Drought Tolerance by Interacting With the ABA Signaling Component BnaA10.ABF2
Drought is a major threat to plant growth and crop productivity. Reduced level of the gibberellin would result in increased drought tolerance, but the underlying mechanism is still unclear. In Brassica napus, there are four BnaRGA genes that code for DELLA proteins, negative regulators of GA signali...
Main Authors: | , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2020-05-01
|
Series: | Frontiers in Plant Science |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.00577/full |
id |
doaj-fc2437b3908842f5b542f46a790429ea |
---|---|
record_format |
Article |
spelling |
doaj-fc2437b3908842f5b542f46a790429ea2020-11-25T02:31:32ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-05-011110.3389/fpls.2020.00577527615Roles of the Brassica napus DELLA Protein BnaA6.RGA, in Modulating Drought Tolerance by Interacting With the ABA Signaling Component BnaA10.ABF2Jiajing Wu0Guanbo Yan1Zhiqiang Duan2Zhijuan Wang3Chunying Kang4Liang Guo5Kede Liu6Jinxing Tu7Jinxiong Shen8Bin Yi9Tingdong Fu10Xia Li11Chaozhi Ma12Cheng Dai13National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaDrought is a major threat to plant growth and crop productivity. Reduced level of the gibberellin would result in increased drought tolerance, but the underlying mechanism is still unclear. In Brassica napus, there are four BnaRGA genes that code for DELLA proteins, negative regulators of GA signaling. Among them, expression of BnaA6.RGA was greatly induced by drought and abscisic acid (ABA). Previously, we created the gain-of-function mutant of BnaA6.RGA, bnaa6.rga-D, and the loss-of-function quadruple mutant, bnarga by CRISPR/Cas9, respectively. Here we show that bnaa6.rga-D displayed enhanced drought tolerance, and its stomatal closure was hypersensitive to ABA treatment. By contrast, bnarga displayed reduced drought tolerance and was less sensitive to ABA treatment, but there is no difference in drought tolerance between single BnaRGA mutant and WT, suggesting a functional redundancy between the BnaRGA genes in this process. Furthermore, we found that BnaRGAs were able to interact physically with BnaA10.ABF2, an essential transcription factor in ABA signaling. The BnaA10.ABF2-BnaA6.RGA protein complex greatly increased the expression level of the drought responsive gene BnaC9.RAB18. Taken together, this work highlighted the fundamental roles of DELLA proteins in drought tolerance in B. napus, and provide desirable germplasm for further breeding of drought tolerance in rapeseed.https://www.frontiersin.org/article/10.3389/fpls.2020.00577/fullGADELLA proteinBnaRGAABABnaA10.ABF2Brassica napus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jiajing Wu Guanbo Yan Zhiqiang Duan Zhijuan Wang Chunying Kang Liang Guo Kede Liu Jinxing Tu Jinxiong Shen Bin Yi Tingdong Fu Xia Li Chaozhi Ma Cheng Dai |
spellingShingle |
Jiajing Wu Guanbo Yan Zhiqiang Duan Zhijuan Wang Chunying Kang Liang Guo Kede Liu Jinxing Tu Jinxiong Shen Bin Yi Tingdong Fu Xia Li Chaozhi Ma Cheng Dai Roles of the Brassica napus DELLA Protein BnaA6.RGA, in Modulating Drought Tolerance by Interacting With the ABA Signaling Component BnaA10.ABF2 Frontiers in Plant Science GA DELLA protein BnaRGA ABA BnaA10.ABF2 Brassica napus |
author_facet |
Jiajing Wu Guanbo Yan Zhiqiang Duan Zhijuan Wang Chunying Kang Liang Guo Kede Liu Jinxing Tu Jinxiong Shen Bin Yi Tingdong Fu Xia Li Chaozhi Ma Cheng Dai |
author_sort |
Jiajing Wu |
title |
Roles of the Brassica napus DELLA Protein BnaA6.RGA, in Modulating Drought Tolerance by Interacting With the ABA Signaling Component BnaA10.ABF2 |
title_short |
Roles of the Brassica napus DELLA Protein BnaA6.RGA, in Modulating Drought Tolerance by Interacting With the ABA Signaling Component BnaA10.ABF2 |
title_full |
Roles of the Brassica napus DELLA Protein BnaA6.RGA, in Modulating Drought Tolerance by Interacting With the ABA Signaling Component BnaA10.ABF2 |
title_fullStr |
Roles of the Brassica napus DELLA Protein BnaA6.RGA, in Modulating Drought Tolerance by Interacting With the ABA Signaling Component BnaA10.ABF2 |
title_full_unstemmed |
Roles of the Brassica napus DELLA Protein BnaA6.RGA, in Modulating Drought Tolerance by Interacting With the ABA Signaling Component BnaA10.ABF2 |
title_sort |
roles of the brassica napus della protein bnaa6.rga, in modulating drought tolerance by interacting with the aba signaling component bnaa10.abf2 |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2020-05-01 |
description |
Drought is a major threat to plant growth and crop productivity. Reduced level of the gibberellin would result in increased drought tolerance, but the underlying mechanism is still unclear. In Brassica napus, there are four BnaRGA genes that code for DELLA proteins, negative regulators of GA signaling. Among them, expression of BnaA6.RGA was greatly induced by drought and abscisic acid (ABA). Previously, we created the gain-of-function mutant of BnaA6.RGA, bnaa6.rga-D, and the loss-of-function quadruple mutant, bnarga by CRISPR/Cas9, respectively. Here we show that bnaa6.rga-D displayed enhanced drought tolerance, and its stomatal closure was hypersensitive to ABA treatment. By contrast, bnarga displayed reduced drought tolerance and was less sensitive to ABA treatment, but there is no difference in drought tolerance between single BnaRGA mutant and WT, suggesting a functional redundancy between the BnaRGA genes in this process. Furthermore, we found that BnaRGAs were able to interact physically with BnaA10.ABF2, an essential transcription factor in ABA signaling. The BnaA10.ABF2-BnaA6.RGA protein complex greatly increased the expression level of the drought responsive gene BnaC9.RAB18. Taken together, this work highlighted the fundamental roles of DELLA proteins in drought tolerance in B. napus, and provide desirable germplasm for further breeding of drought tolerance in rapeseed. |
topic |
GA DELLA protein BnaRGA ABA BnaA10.ABF2 Brassica napus |
url |
https://www.frontiersin.org/article/10.3389/fpls.2020.00577/full |
work_keys_str_mv |
AT jiajingwu rolesofthebrassicanapusdellaproteinbnaa6rgainmodulatingdroughttolerancebyinteractingwiththeabasignalingcomponentbnaa10abf2 AT guanboyan rolesofthebrassicanapusdellaproteinbnaa6rgainmodulatingdroughttolerancebyinteractingwiththeabasignalingcomponentbnaa10abf2 AT zhiqiangduan rolesofthebrassicanapusdellaproteinbnaa6rgainmodulatingdroughttolerancebyinteractingwiththeabasignalingcomponentbnaa10abf2 AT zhijuanwang rolesofthebrassicanapusdellaproteinbnaa6rgainmodulatingdroughttolerancebyinteractingwiththeabasignalingcomponentbnaa10abf2 AT chunyingkang rolesofthebrassicanapusdellaproteinbnaa6rgainmodulatingdroughttolerancebyinteractingwiththeabasignalingcomponentbnaa10abf2 AT liangguo rolesofthebrassicanapusdellaproteinbnaa6rgainmodulatingdroughttolerancebyinteractingwiththeabasignalingcomponentbnaa10abf2 AT kedeliu rolesofthebrassicanapusdellaproteinbnaa6rgainmodulatingdroughttolerancebyinteractingwiththeabasignalingcomponentbnaa10abf2 AT jinxingtu rolesofthebrassicanapusdellaproteinbnaa6rgainmodulatingdroughttolerancebyinteractingwiththeabasignalingcomponentbnaa10abf2 AT jinxiongshen rolesofthebrassicanapusdellaproteinbnaa6rgainmodulatingdroughttolerancebyinteractingwiththeabasignalingcomponentbnaa10abf2 AT binyi rolesofthebrassicanapusdellaproteinbnaa6rgainmodulatingdroughttolerancebyinteractingwiththeabasignalingcomponentbnaa10abf2 AT tingdongfu rolesofthebrassicanapusdellaproteinbnaa6rgainmodulatingdroughttolerancebyinteractingwiththeabasignalingcomponentbnaa10abf2 AT xiali rolesofthebrassicanapusdellaproteinbnaa6rgainmodulatingdroughttolerancebyinteractingwiththeabasignalingcomponentbnaa10abf2 AT chaozhima rolesofthebrassicanapusdellaproteinbnaa6rgainmodulatingdroughttolerancebyinteractingwiththeabasignalingcomponentbnaa10abf2 AT chengdai rolesofthebrassicanapusdellaproteinbnaa6rgainmodulatingdroughttolerancebyinteractingwiththeabasignalingcomponentbnaa10abf2 |
_version_ |
1724823887892447232 |