Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog

Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog

We previously reported the non-viral derivation of transgene-free induced pluripotent stem cells (iPSCs) from somatic fibroblasts of a female beagle dog using an optimized induction medium and integration-free episomal vectors. Here, we report novel derivation of a male canine iPSC line OF35Y-iPS, w...

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Main Authors: Sho Yoshimatsu, Kazuya Edamura, Yumi Yoshii, Aozora Iguchi, Hirotaka Kondo, Hisashi Shibuya, Tsukika Sato, Seiji Shiozawa, Hideyuki Okano
Format: Article
Language:English
Published: Elsevier 2021-05-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S187350612100221X
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spelling doaj-fc2a5e338510425c8aa2ffbd9c48749e2021-05-30T04:41:50ZengElsevierStem Cell Research1873-50612021-05-0153102375Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dogSho Yoshimatsu0Kazuya Edamura1Yumi Yoshii2Aozora Iguchi3Hirotaka Kondo4Hisashi Shibuya5Tsukika Sato6Seiji Shiozawa7Hideyuki Okano8Department of Physiology, School of Medicine, Keio University, Tokyo, JapanLaboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kanagawa, JapanLaboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kanagawa, JapanLaboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kanagawa, JapanLaboratory of Veterinary Pathology, College of Bioresource Sciences, Nihon University, Kanagawa, JapanLaboratory of Veterinary Pathology, College of Bioresource Sciences, Nihon University, Kanagawa, JapanDepartment of Physiology, School of Medicine, Keio University, Tokyo, JapanDepartment of Physiology, School of Medicine, Keio University, Tokyo, JapanDepartment of Physiology, School of Medicine, Keio University, Tokyo, Japan; Corresponding author at: Department of Physiology, School of Medicine, Keio University, Shinjuku, Tokyo, Japan.We previously reported the non-viral derivation of transgene-free induced pluripotent stem cells (iPSCs) from somatic fibroblasts of a female beagle dog using an optimized induction medium and integration-free episomal vectors. Here, we report novel derivation of a male canine iPSC line OF35Y-iPS, which showed standard characteristics of pluripotency such as a strong gene expression profile of pluripotency markers, differentiation potential into all three germ layers, and normal karyotype (78XY). Furthermore, we demonstrated targeted integration of 2A-EGFP into the canine NANOS3 locus. The novel iPSC line would be a useful resource for stem cell research and regenerative veterinary medicine.http://www.sciencedirect.com/science/article/pii/S187350612100221X
collection DOAJ
language English
format Article
sources DOAJ
author Sho Yoshimatsu
Kazuya Edamura
Yumi Yoshii
Aozora Iguchi
Hirotaka Kondo
Hisashi Shibuya
Tsukika Sato
Seiji Shiozawa
Hideyuki Okano
spellingShingle Sho Yoshimatsu
Kazuya Edamura
Yumi Yoshii
Aozora Iguchi
Hirotaka Kondo
Hisashi Shibuya
Tsukika Sato
Seiji Shiozawa
Hideyuki Okano
Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog
Stem Cell Research
author_facet Sho Yoshimatsu
Kazuya Edamura
Yumi Yoshii
Aozora Iguchi
Hirotaka Kondo
Hisashi Shibuya
Tsukika Sato
Seiji Shiozawa
Hideyuki Okano
author_sort Sho Yoshimatsu
title Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog
title_short Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog
title_full Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog
title_fullStr Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog
title_full_unstemmed Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog
title_sort non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2021-05-01
description We previously reported the non-viral derivation of transgene-free induced pluripotent stem cells (iPSCs) from somatic fibroblasts of a female beagle dog using an optimized induction medium and integration-free episomal vectors. Here, we report novel derivation of a male canine iPSC line OF35Y-iPS, which showed standard characteristics of pluripotency such as a strong gene expression profile of pluripotency markers, differentiation potential into all three germ layers, and normal karyotype (78XY). Furthermore, we demonstrated targeted integration of 2A-EGFP into the canine NANOS3 locus. The novel iPSC line would be a useful resource for stem cell research and regenerative veterinary medicine.
url http://www.sciencedirect.com/science/article/pii/S187350612100221X
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