Possibility of micropropagation of dianthus giganteiformis subsp. Kladovanus (Degen) SÓO by the method of proliferation of lateral shoots

• Main Authors: Marković Marija, Grbić Mihailo, Šindelić Ana
• Format: Article
• Language: srp
• Published: Faculty of Forestry, Belgrade 2006-01-01
• Series: Glasnik Šumarskog Fakulteta: Univerzitet u Beogradu
• Subjects: kladovanus carnation; endangered species; micropropagation
• Online Access: http://www.doiserbia.nb.rs/img/doi/0353-4537/2006/0353-45370694171M.pdf

The micropropagation of kladovanus carnation (Dianthus giganteiformis subsp kladovanus) was tested by the lateral shoot proliferation method. Micropropagation was performed on the medium with 1/2 MS mineral solution supplemented with 3% sucrose, 0.8% agar, 50 mg·L-1 mio-inositol, 0.025 mg·L-1 thiamin, 0.125 mg·L-1 nicotinic acid and 0.5 mg·L-1 glycine. The development of lateral shoots was induced on 6-benzyl-aminopurine (BAP) and α-naphthaleneacetic acid (NAA). The best results were achieved on the medium with 1 mg·L-1 BAP and 0.1 mg·L-1 NAA. Rooting was successful on the medium without hormones, and the percentage of acclimatisation was 83%.