Improvement in the definition of anti-HLA antibody profile in highly sensitized patients.
The definition of anti-HLA antibody profile in highly sensitized patients on a waiting list is crucial when virtual crossmatch is used in organ allocation systems, but also when used to identify the true deleterious anti-HLA antibodies. Here we propose different levels of risk based on the results o...
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doaj-fc3d66d6508d41d893ca6e73f2df31432020-11-25T00:02:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e017146310.1371/journal.pone.0171463Improvement in the definition of anti-HLA antibody profile in highly sensitized patients.Juan IrureEsther AsensioEmilio RodrigoÍñigo RomónJavier GómezManuel AriasMarcos López-HoyosDavid San SegundoThe definition of anti-HLA antibody profile in highly sensitized patients on a waiting list is crucial when virtual crossmatch is used in organ allocation systems, but also when used to identify the true deleterious anti-HLA antibodies. Here we propose different levels of risk based on the results of anti-HLA antibody testing in neat serum (N) and after sera dilution (DIL) and C1q test in 18 highly sensitized patients. This group was heterogeneous in terms of anti-HLA antibody titers and their ability to fix complement. After dilution, 15 out of 18 patients (83.3%) showed a reduction of positive bead counts whereas 4 patients showed a prozone effect and complement fixation was demonstrated. The high dilution of sera and ascertaining the complement fixation allow the accurate definition of risk anti-HLA antibody profiles in highly sensitized patients, as demonstrated in 5 of the sensitized patients who were transplanted.http://europepmc.org/articles/PMC5291387?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juan Irure Esther Asensio Emilio Rodrigo Íñigo Romón Javier Gómez Manuel Arias Marcos López-Hoyos David San Segundo |
spellingShingle |
Juan Irure Esther Asensio Emilio Rodrigo Íñigo Romón Javier Gómez Manuel Arias Marcos López-Hoyos David San Segundo Improvement in the definition of anti-HLA antibody profile in highly sensitized patients. PLoS ONE |
author_facet |
Juan Irure Esther Asensio Emilio Rodrigo Íñigo Romón Javier Gómez Manuel Arias Marcos López-Hoyos David San Segundo |
author_sort |
Juan Irure |
title |
Improvement in the definition of anti-HLA antibody profile in highly sensitized patients. |
title_short |
Improvement in the definition of anti-HLA antibody profile in highly sensitized patients. |
title_full |
Improvement in the definition of anti-HLA antibody profile in highly sensitized patients. |
title_fullStr |
Improvement in the definition of anti-HLA antibody profile in highly sensitized patients. |
title_full_unstemmed |
Improvement in the definition of anti-HLA antibody profile in highly sensitized patients. |
title_sort |
improvement in the definition of anti-hla antibody profile in highly sensitized patients. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
The definition of anti-HLA antibody profile in highly sensitized patients on a waiting list is crucial when virtual crossmatch is used in organ allocation systems, but also when used to identify the true deleterious anti-HLA antibodies. Here we propose different levels of risk based on the results of anti-HLA antibody testing in neat serum (N) and after sera dilution (DIL) and C1q test in 18 highly sensitized patients. This group was heterogeneous in terms of anti-HLA antibody titers and their ability to fix complement. After dilution, 15 out of 18 patients (83.3%) showed a reduction of positive bead counts whereas 4 patients showed a prozone effect and complement fixation was demonstrated. The high dilution of sera and ascertaining the complement fixation allow the accurate definition of risk anti-HLA antibody profiles in highly sensitized patients, as demonstrated in 5 of the sensitized patients who were transplanted. |
url |
http://europepmc.org/articles/PMC5291387?pdf=render |
work_keys_str_mv |
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