Docosahexaenoic acid provides protective mechanism in bilaterally MPTP-lesioned rat model of Parkinson's disease
Docosahexaenoic acid (DHA), a major polyunsaturated fatty acid (PUFA) in the phospholipid fraction of the brain, is essential for normal cellular function. Neurodegenerative disorders such as Parkinson’s disease (PD) often exhibit significant declines in PUFAs. The aim of this study was to observe t...
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Via Medica
2012-07-01
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doaj-fc411089022845b89db3b7d5783fe3d02020-11-24T22:15:06ZengVia MedicaFolia Histochemica et Cytobiologica0239-85081897-56312012-07-0150222823810.5603/FHC.2012.0032Docosahexaenoic acid provides protective mechanism in bilaterally MPTP-lesioned rat model of Parkinson's diseaseGulay HaciogluYasemin Seval-CelikGamze TanrioverOzlem OzsoyEsen Saka-TopcuogluSevin BalkanAysel AgarDocosahexaenoic acid (DHA), a major polyunsaturated fatty acid (PUFA) in the phospholipid fraction of the brain, is essential for normal cellular function. Neurodegenerative disorders such as Parkinson’s disease (PD) often exhibit significant declines in PUFAs. The aim of this study was to observe the effects of DHA supplementation in an experimental rat model of PD created with ‘1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine’ (MPTP). Adult male Wistar rats were divided into four groups: (1) Control; (2) DHA-treated; (3) MPTP-induced; and (4) MPTP-induced + DHA-treated. Motor activity was investigated using the ‘vertical pole’ and ‘vertical wire’ tests. The dopaminergic lesion was determined by immunohistochemical analysis for tyrosine hydroxylase (TH)-immunopositive cells in substantia nigra (SN). Immunoreactivities of Bcl-2, Akt and phosphorylated-Akt (p-Akt) in SN were evaluated by immunohistochemistry. MPTP-induced animals exhibited decreased locomotor activity, motor coordination and loss of equilibrium. Diminished Parkinsonism symptoms and decreased dopaminergic neuron death were detected in the MPTP-induced + DHA-treated group compared to the MPTP-induced group. Moderate decreases in Akt staining were found in the MPTP-induced and MPTP-induced + DHA-treated groups compared to controls. p-Akt immunoreactivity decreased dramatically in the MPTP-induced group compared to the control; however, it was increased in the MPTP-induced + DHA-treated group compared to the MPTP-induced group. The staining intensity for Bcl-2 decreased prominently in the MPTP-induced group compared to the control, while it was stronger in the MPTP-induced + DHA-treated group compared to the MPTP-induced group. In conclusion, DHA significantly protects dopaminergic neurons against cell death in an experimental PD model. Akt/p-Akt and Bcl-2 pathways are related to this protective effect of DHA in experimental PD.http://czasopisma.viamedica.pl/fhc/article/view/15781Parkinson’s diseaseMPTPDocosahexaenoic acidDopaminergic neuron survivalAkt/p-AktBcl-2Rat |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gulay Hacioglu Yasemin Seval-Celik Gamze Tanriover Ozlem Ozsoy Esen Saka-Topcuoglu Sevin Balkan Aysel Agar |
spellingShingle |
Gulay Hacioglu Yasemin Seval-Celik Gamze Tanriover Ozlem Ozsoy Esen Saka-Topcuoglu Sevin Balkan Aysel Agar Docosahexaenoic acid provides protective mechanism in bilaterally MPTP-lesioned rat model of Parkinson's disease Folia Histochemica et Cytobiologica Parkinson’s disease MPTP Docosahexaenoic acid Dopaminergic neuron survival Akt/p-Akt Bcl-2 Rat |
author_facet |
Gulay Hacioglu Yasemin Seval-Celik Gamze Tanriover Ozlem Ozsoy Esen Saka-Topcuoglu Sevin Balkan Aysel Agar |
author_sort |
Gulay Hacioglu |
title |
Docosahexaenoic acid provides protective mechanism in bilaterally MPTP-lesioned rat model of Parkinson's disease |
title_short |
Docosahexaenoic acid provides protective mechanism in bilaterally MPTP-lesioned rat model of Parkinson's disease |
title_full |
Docosahexaenoic acid provides protective mechanism in bilaterally MPTP-lesioned rat model of Parkinson's disease |
title_fullStr |
Docosahexaenoic acid provides protective mechanism in bilaterally MPTP-lesioned rat model of Parkinson's disease |
title_full_unstemmed |
Docosahexaenoic acid provides protective mechanism in bilaterally MPTP-lesioned rat model of Parkinson's disease |
title_sort |
docosahexaenoic acid provides protective mechanism in bilaterally mptp-lesioned rat model of parkinson's disease |
publisher |
Via Medica |
series |
Folia Histochemica et Cytobiologica |
issn |
0239-8508 1897-5631 |
publishDate |
2012-07-01 |
description |
Docosahexaenoic acid (DHA), a major polyunsaturated fatty acid (PUFA) in the phospholipid fraction of the brain, is essential for normal cellular function. Neurodegenerative disorders such as Parkinson’s disease (PD) often exhibit significant declines in PUFAs. The aim of this study was to observe the effects of DHA supplementation in an experimental rat model of PD created with ‘1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine’ (MPTP). Adult male Wistar rats were divided into four groups: (1) Control; (2) DHA-treated; (3) MPTP-induced; and (4) MPTP-induced + DHA-treated. Motor activity was investigated using the ‘vertical pole’ and ‘vertical wire’ tests. The dopaminergic lesion was determined by immunohistochemical analysis for tyrosine hydroxylase (TH)-immunopositive cells in substantia nigra (SN). Immunoreactivities of Bcl-2, Akt and phosphorylated-Akt (p-Akt) in SN were evaluated by immunohistochemistry. MPTP-induced animals exhibited decreased locomotor activity, motor coordination and loss of equilibrium. Diminished Parkinsonism symptoms and decreased dopaminergic neuron death were detected in the MPTP-induced + DHA-treated group compared to the MPTP-induced group. Moderate decreases in Akt staining were found in the MPTP-induced and MPTP-induced + DHA-treated groups compared to controls. p-Akt immunoreactivity decreased dramatically in the MPTP-induced group compared to the control; however, it was increased in the MPTP-induced + DHA-treated group compared to the MPTP-induced group. The staining intensity for Bcl-2 decreased prominently in the MPTP-induced group compared to the control, while it was stronger in the MPTP-induced + DHA-treated group compared to the MPTP-induced group. In conclusion, DHA significantly protects dopaminergic neurons against cell death in an experimental PD model. Akt/p-Akt and Bcl-2 pathways are related to this protective effect of DHA in experimental PD. |
topic |
Parkinson’s disease MPTP Docosahexaenoic acid Dopaminergic neuron survival Akt/p-Akt Bcl-2 Rat |
url |
http://czasopisma.viamedica.pl/fhc/article/view/15781 |
work_keys_str_mv |
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